FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_39.35100000043c13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_39.35100000043c13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196312
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC69463.5382452422674113No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT11730.5975182362769469No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3440.1752312645177065No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCG2690.13702677370716002No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA2480.12632951628020703No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC2480.12632951628020703No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2270.115632258853254No Hit
AATACATACAGTGCAATTGTTATTACATTCGAGGTTTGGATTTATATCTAG2200.11206650637760299No Hit
GAGGAAAAGGAGAAGAAGGATAAGGAAAGGAAGGAAAAGGAGAAGAAGGAT2160.11002893353437386No Hit
GGATAAGGAAAGGAAGGAAAAGGAGAAGAAGGATAAACTGGAAAAGGAAAA2120.10799136069114472No Hit
GAAGGAGAAGGAGAAAAAGGATAAGGAGGAAAAGGAGAAGAAGGATAAGGA2110.10748196748033743No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC2080.10595378784791556No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2060.104935001426301No Hit
CTTTATTACTTATGCTAGCGAGCGGATACAAACAACACACAATATAGTTAC2050.10442560821549371No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC6500.043.958345
GGCAATG250.002103623535.99735320
ATGCCGT7950.035.94075444
TATGCCG8600.033.224343
GTATGCC8600.033.224342
CGTATGC8700.033.10101341
TCTCGTA8850.032.8025538
TATCTCG8800.032.73320436
TCGTATG8800.032.72486540
CTGCGAC8550.032.62917728
GACTGCG8650.032.5120626
TGCGACC8600.032.43947229
CTCGTAT8950.032.4277839
AGACTGC8700.032.32520725
ATCTCGT8950.032.184637
TGACCAT352.8131346E-432.1404910
CACGAGA9100.031.64602321
ACTGCGA8850.031.52310427
ACGAGAC9150.031.47309322
GACCTAT9050.031.3316432
Produced by FastQC (version 0.11.2)