FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_37.35100000043bde.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_37.35100000043bde.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284239
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC6441022.66050753063443No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT268129.432906814335823No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCG45711.60815370163841No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT12850.45208433747656024No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA10910.38383191609877604No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC10890.38312828288869577No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA10420.3665929024518099No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA10270.361315653376208No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT10140.35674203751068645No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA9110.3205049271915536No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC8590.3022104637294671No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC7580.2666769866204145No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACCTGTCTCTTA6580.23149532611640203No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCT6260.22023719475511805No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTAT6250.21988537815007791No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTATA6080.2139044958643958No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTC5930.20862724678879394No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC5760.2026463645031118No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC5540.19490639919222907No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA5440.19138823314182782No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTCT5060.1780192021503031No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCCTGTCTCTTATACACATCTC5010.17626011912510248No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTATA4790.16852015381421973No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTAT4610.16218745492349748No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT4560.16042837189829687No Hit
GTATCAACGCAGAGTACGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA4460.1569102058478956No Hit
GTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCACGA4360.15339203979749436No Hit
GAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTC4170.146707524301732No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC4090.143892991461411No Hit
AAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCACG3770.132634860100127No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCTC3640.12806124423460538No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC3620.12735761102452514No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACTGTCTCTTAT3540.12454307818420415No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCT3530.124191261579164No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCCTGTCTCT3530.124191261579164No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACCTGTCTCTTATACACATCTCCG3460.12172854534388314No Hit
GGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCACG3360.11821037929348191No Hit
AAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACTGTCTCTTATAC3300.11609947966324116No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC3130.11011859737755902No Hit
GGTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCT3010.10589679811707753No Hit
GTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCG2970.10448953169691702No Hit
TATCAACGCAGAGTACGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTAT2970.10448953169691702No Hit
AAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACCTGTCTCTTATA2960.1041377150918769No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCA207.0254E-444.99859242
ACCTATT207.0254E-444.99859233
TGCAGTC207.0254E-444.99859245
TGCCGTC67600.044.3662245
TAAAAGT659.094947E-1238.0958371
CCTATTC250.002104769735.9988734
ATGCCGT96150.031.19247244
GAAAAGT651.3889803E-831.1693211
TATGCAG300.005141603829.9990643
ACCTATC100250.029.91676933
GACCTAT100350.029.90937832
CGACCTA100400.029.89448231
ATCTCGT100800.029.88745937
CCTATCT100200.029.88679134
CTATCTC100300.029.87942535
TCTCGTA100950.029.86533538
CTGCGAC100950.029.86533528
CGAGACT101500.029.85867123
TATCTCG100800.029.84281736
ACTGCGA101050.029.83578127
Produced by FastQC (version 0.11.2)