FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_35.35100000043ba8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_35.35100000043ba8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128423
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC2098216.338194871635142No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT1293810.074519361796563No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCG15411.1999408205695241No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCG1480.11524415408454873No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACA207.009875E-445.01
TGCCGTC22500.044.145
GCTTATC250.002100159936.020
GTACAAT401.5498377E-533.753
GCCGTCT401.5498377E-533.7545
TGTCTGA300.00513044529.99999830
ATCGATT300.00513044529.99999842
ATGCCGT36350.027.29711244
TACAATC608.117737E-626.2499984
TATGCCG38550.025.79766543
CGTATGC38750.025.66451541
TCGTATG38800.025.57345440
CTCGTAT38750.025.54838639
GTATGCC38850.025.5405442
GCCCACG38950.025.53273418
ACCTATC38900.025.50771133
ATCTCGT38750.025.4903237
CCGAGCC39300.025.47709814
CCCACGA38950.025.47496819
ACGAGAC39050.025.46735222
Produced by FastQC (version 0.11.2)