FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_32.35100000043b3e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_32.35100000043b3e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225104
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC147416.548528680076765No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT30041.3344942782002984No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCG4770.21190205416163196No Hit
TCTAAGGAGAGAGTTATAAAGATGGGAAAGAAGAAAACCTGTACTGTTAGA3010.13371597128438412No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTTA207.0230674E-444.9975615
GTCGATA302.1585074E-644.9975638
CCTACGC207.0230674E-444.9975626
TGGTTCC207.0230674E-444.9975626
GAGCGAA207.0230674E-444.9975611
GTCGTGT207.0230674E-444.9975624
ACTCGTG207.0230674E-444.997567
TCCGTCT207.0230674E-444.9975631
GTCCCAT207.0230674E-444.9975631
TCCCCTA207.0230674E-444.9975635
GCTAAGG207.0230674E-444.9975630
GACTCGT207.0230674E-444.997566
GACTCCC207.0230674E-444.9975625
GAGTGTG253.8820264E-544.9975557
GCTGAGA253.8820264E-544.99755522
ACGGCTA253.8820264E-544.99755544
ACGAGTG253.8820264E-544.99755511
AGTCGTC253.8820264E-544.99755535
TGCCGTC16800.043.12265845
ACTGCAC451.9173967E-839.99782618
Produced by FastQC (version 0.11.2)