FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_30.35100000043af9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_30.35100000043af9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358960
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC19137653.314018275016714No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT3971711.064464007131713No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCG141853.9516937820369953No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACA6680.18609315801203477No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACA6610.18414308000891463No Hit
GTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTC6280.17494985513706263No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACCTGTCTC5910.1646422999777134No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC5430.15127033652774682No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATAC4690.13065522620904835No Hit
GTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCT4020.11199019389347001No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACCTGTCT3600.10028972587474928No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTCGC207.024975E-445.0023727
AGACCTC207.024975E-445.0023725
GACCTCG207.024975E-445.0023726
CTCGCAG207.024975E-445.0023729
TGCGGTC302.1626056E-644.996145
ATCTACG253.8874183E-544.99609810
TGCCGTC202200.044.67342845
GCCGTCT1400.041.7820945
ATGCCGT238750.037.8061544
GAATTCG301.13800575E-437.50197235
CAGAATT601.546141E-1037.50197233
TTTCGTA301.13800575E-437.50197238
GTATGCT601.546141E-1037.4967542
CATCTAC301.13893955E-437.496759
TCTACGA301.13893955E-437.4967511
ACGAGCC301.13893955E-437.4967514
CGAGCCA301.13893955E-437.4967515
TCGCAGA250.00210463936.00189230
TAAAGCA250.00210463936.0018924
TGCCGAC250.00210607935.9968845
Produced by FastQC (version 0.11.2)