H23LNBCXX_l02n01_bir_0113_pa_3.351000000437ac.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l02n01_bir_0113_pa_3.351000000437ac.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	321644
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC	2788	0.8667968312793026	RNA PCR Primer, Index 27 (95% over 21bp)
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC	749	0.23286615015358594	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGT	455	0.14146074542040268	No Hit
TATTAGCTCTAGAATTACTACGGTTATCCGAGTAGTAGTTACCATCAAACA	363	0.11285769359913445	No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA	349	0.10850505527850668	No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC	342	0.1063287361181928	No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG	334	0.10384151422069121	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	420	0.0	36.960583	43
GTATGCC	430	0.0	36.624237	42
ATGCCGT	425	0.0	36.525753	44
CGTATGC	435	0.0	36.203266	41
CTCGTAT	440	0.0	35.791866	39
TCGTATG	440	0.0	35.791866	40
TGCCGTC	450	0.0	34.99649	45
GCTATCT	445	0.0	34.884144	34
CGAGACG	445	0.0	34.884144	23
CACGAGA	455	0.0	34.611916	21
GCCCACG	455	0.0	34.11746	18
ATCTCGT	465	0.0	33.867573	37
CCCACGA	465	0.0	33.867573	19
TGCTATC	460	0.0	33.746616	33
GAATTGC	460	0.0	33.257538	29
ATTGCTA	465	0.0	32.89993	31
GACGAAT	465	0.0	32.89993	26
CGCGCGA	55	1.1244083E-7	32.72399	10
TCTCGTA	485	0.0	32.470974	38
TTGCTAT	480	0.0	31.871807	32