FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_29.35100000043adf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_29.35100000043adf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188117
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC12511066.50648266770148No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT2274412.090348028088902No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCG74733.9725277353987147No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCTGTC2020.10737998160719125No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTTAT206.984578E-445.0439031
TTTATAC253.862114E-545.031923
GCCCCAC207.0212974E-444.99601418
AGCCCCA207.0212974E-444.99601417
GTATGCT253.8803628E-544.99601442
GTATGCG253.8803628E-544.99601442
CTCCCGA207.0212974E-444.99601412
ATGCGGT253.8803628E-544.99601444
CCTTCCA207.0212974E-444.99601428
CCTTACA207.0212974E-444.99601428
GACCTTA253.8803628E-544.99601426
TGCGGTC253.8803628E-544.99601445
TATGCTG302.1571122E-644.9960143
TGCCGTC130700.044.49682245
TATGCGG301.1368889E-437.49667443
CCGTCTT301.1368889E-437.49667445
ACCTTAC301.1368889E-437.49667427
ATGCCGT155800.037.28488544
CTTTATA250.002095331236.025542
ATCTCCC250.002103555535.9968110
Produced by FastQC (version 0.11.2)