FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_28.35100000043ab5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_28.35100000043ab5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455599
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC202434.4431616399509215No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT42900.9416175189146596No Hit
ATATACAGCCTGTTGAATCAAATAGACAGGTATGAGCCAACCTAGCGAACT13880.3046538732525752No Hit
ACATAAAAGCAAAACTATAGGCTGTCTTTACTTAGCAAATAAGCAGACTAG9960.21861329809766922No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC9390.20610229609810382No Hit
GGATACAGCTAGTAGCTTTCCACTTTCGACTACAGGACTTTTACCTTCTTT7850.1723006415729622No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCG7280.15978963957339679No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC6140.13476763557426596No Hit
AGATAAATCTATCGGTTTAGGCTCTTCCCCTTTCGCTCACCACTACTTGGG6010.13191424915331246No Hit
ACTTGGGGAATCTCGTTTGATTTCTTTTCCTCTAGCTACTAAGATGTTTCA5550.12181765104840002No Hit
GGTAATTTTGGCTTAACGTGTTTAGAGCTTTTCTTGGAAGCTTGATTGTTT5400.11852528210114596No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA5320.1167693519959438No Hit
CCCGATATACAGCCTGTTGAATCAAATAGACAGGTATGAGCCAACCTAGCG5310.11654986073279354No Hit
CCGATATACAGCCTGTTGAATCAAATAGACAGGTATGAGCCAACCTAGCGA4940.10842868399623352No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC4860.10667275389103137No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC4780.10491682378582921No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT4660.10228292862802596No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTGGA207.0184993E-445.0133931
TGCCGTC21900.043.9712145
GCCGTCT451.9244908E-839.99873445
ATGCCGT26100.036.89538244
CGTAAAG250.00210610435.9988640
CCTTGCC250.00210610435.9988628
TATGCCG26900.035.79812243
GCAGAAT26800.035.76379432
ACGAGAC27150.035.71710222
CTCGTAT27100.035.69997439
CAGAATC27000.035.66553533
AGACCTT27150.035.6342325
TCGTATG27150.035.6342340
TGCAGAA26850.035.61339631
CGTATGC27200.035.56872641
GACCTTG27150.035.5513626
CCACGAG27350.035.45591420
CCTTGCA27100.035.3678828
GAGACCT27550.035.28018624
TTGCAGA27300.035.0263630