FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_25.35100000043a65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_25.35100000043a65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences678659
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC96491.4217744110075898No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT20710.30516061821916457No Hit
CCATATGACTTAGCCATTTTCAGTTTGGGATATTGTTATCCAAAGAACCAA13630.2008372393204835No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC10900.041.48810645
TCAGCCC601.546141E-1037.49754714
ATGCCGT13650.033.29452544
TAAGGGT551.1250086E-732.7323654
CGTATGC14350.031.98396741
TATGCCG14250.031.89264943
ACGAGAC14750.031.42398622
CACGAGA14850.031.36389421
GTATGCC14600.031.282242
CGAGACC14850.031.0608623
CCCACGA14950.031.00359719
CTCGTAT14850.030.90706639
TCGTATG15050.030.64583240
TCTCGTA15200.030.19761538
GCCCACG15500.030.04863518
ATCTCGT15150.030.00024637
GCAGAAT15150.030.00024632
AATCTCG15300.029.70612536
CCTTGCA15450.029.56334928
CTTGCAG15550.029.51792329
Produced by FastQC (version 0.11.2)