FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_24.35100000043a4c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_24.35100000043a4c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163377
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC135398.286968177895297No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT36432.229812029845082No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG5980.3660245934250231No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGATT207.006103E-445.012253
TGCCGTC13800.044.3463245
ATGCTAG250.002099025536.0097961
AAGCAAC250.002099025536.0097963
ATGCCGT17950.034.09354844
AGCCCAC18450.033.2915517
CCCACGA18400.033.2597419
TCCGAGC18550.033.23336813
CGAGACT18350.033.22775323
TATGCCG18450.033.169643
CGTATGC18550.033.1120841
CTCGTAT18550.033.1120839
CACGAGA18500.033.07995621
ACGAGAC18500.033.07995622
CTCCGAG18650.033.05517612
GCCCACG18600.033.0230718
TCTCGTA18600.033.0230738
GAGCCCA18600.033.0230716
CGAGCCC18600.033.0230715
GTATGCC18550.032.9907942
Produced by FastQC (version 0.11.2)