FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_23.35100000043a22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_23.35100000043a22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196256
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC40202.0483450187510193No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT11200.5706831893037665No Hit
TTGTAGTACTTGGCTGATTCTTTCTCATAACGTGTGAGGTTATTTTTGAAA2490.1268751019077124No Hit
GTTACAAAAACAGATTCGGACTCTATTACGTCGATTTCAAAAATAACCTCA2140.10904125224196967No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG2090.10649355943257786No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA1990.10139817381379422No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT1990.10139817381379422No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4000.043.30980345
GCCATCC301.1368925E-437.49766523
TCGCGCG301.1368925E-437.4976659
ACTTGGC900.032.4979748
GTCGCGC352.8129455E-432.1408548
ATGCCGT5550.031.61965444
TATGCCG5750.030.1285643
GTATGCC5800.029.8688342
TATCTCG5750.029.7372836
CGTATGC5850.029.61354341
ACGTGTG1001.8189894E-1229.24817830
GTGAGGT1001.8189894E-1229.24817834
TCGTATG5950.029.11583540
CCTATCT5900.028.98124734
CGAGACT6050.028.63458323
ACGAGAC6050.028.63458322
GCCCACG6100.028.39987218
CACGAGA6100.028.39987221
CTCGTAT6150.028.16897839
CTAGATC6000.028.1232528