FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_19.3510000004399d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_19.3510000004399d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166901
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC65583.929275438733141No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT23721.4212017902828622No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG4300.2576377613076015No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTAG207.01595E-445.01
TGCCGTC8000.042.4687545
TGCTAGA250.002101962736.02
GTACCGC352.8101253E-432.1428576
ATGCCGT10650.031.9014144
TATGCCG10950.031.02739743
GTATGCC10950.031.02739742
CTCGTAT11100.030.8108139
TCGTATG11100.030.8108140
TATCTCG11150.030.67264636
CCTATCT11150.030.67264634
CGTATGC11100.030.60810941
TCTCGTA11200.030.53571338
CTAGATC11200.030.3348228
ATCTCGT11300.030.26548837
CGAGACT11300.030.26548823
TCCTATC11100.030.20270233
ACGAGAC11400.030.19736922
ATCCTAT11200.030.13392832
TAGATCC11200.030.13392829
Produced by FastQC (version 0.11.2)