FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_16.35100000043930.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_16.35100000043930.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75166
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC795210.57925125721736TruSeq Adapter, Index 12 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT13331.7734081898730811No Hit
GACAGAAACTATATACCAAAACAAAACTCCTGATTTTTCCTCAATCTTAAT3750.49889577734614055No Hit
GAAAAAAAAATCGAATCGAAATTAAAAGAATCGAATCGAATCAAAGCATAA3300.4390282840646037No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCG2810.37383923582470796No Hit
TAGTGACATAAAACTTCAAACACTTCTACCAAAATGGAAGGATATTATCAA2520.33525796237660643No Hit
CAACAGATACACAAAAAGCTAGAAATTAAAACGTACCACCAGAAAAAATTA2470.3286060186786579No Hit
AATCAGGAGTCTTTTCACAGAGTATTTCAATTGCAAATCAAAGATAGCGAT1920.25543463800122396No Hit
TTAGAATCATTTTTCGGACATTTGAAAAACCATATCCAAGTTCATCGAGGA1680.22350530825107096No Hit
GTATATACCTTGTGTGAACATTATATGGATTTAGAAGGCATGTATGCACGC1680.22350530825107096No Hit
GTATATAGACTCTAGAAAGGATGCCAAAGAATTGGTTAAAGGCTTAATGGA1660.22084453077189153No Hit
ACGGTACATGATATCAACTTCATGTATGATACCAATAAATCGGCAAGTAAA1590.2115318095947636No Hit
ATATTGCTAACTCTTCTATGGATGAAGATAATTTTGAAGACTTCAAGGTAC1570.20887103211558417No Hit
AATCTGTAGTTGTCTATTGTCTAATCTGCTTTCTAGATCTTTCATCCAATC1530.20354947715722532No Hit
GAGTAGAACAATAACTAAAATAGCCTTGTCATTTCCTTTCTATGCAGTATG1480.1968975334592768No Hit
TAATTCAACAAGTTCATACGGTTGATATTGTTCTTCTAAAGATGATGTTTC1370.18226325732379No Hit
CTGTAATAATTGCCATTTGTTAAGATTGTTAAGTTGAAACAAATTGTTGGG1360.1809328685842003No Hit
ACTTTACATTATGCACAAATTGATGTAGAAAAGTAGCAGTGAAATGAAACG1340.17827209110502087No Hit
ATTTTCAAAAAGCTAGAAGAGAAGATTTTGAATGTTGTCAACACAAAAGTA1240.1649682037091238No Hit
GTAATAATGTACAAGGCTTGCATAAATTATCATCGACTGCTAAGTTAGATT1190.15831626001117524No Hit
CTTACGTTTTGTTTCTACCAATCTTCCTTCACTTAATATCAATGCATGAGC1190.15831626001117524No Hit
GAAGAAGAGAGATTGTTCTTTTATCCTTTTCCAGAAAGATCAGCATACAGG1180.15698587127158556No Hit
ACATGTTTTATACCTTCATACTCTTTCCTTGTGATAACTTGTTTCAGTCTC1140.15166431631322672No Hit
GTATGTTTCATTGTAGAATGTGCTAAGTATTGCCATTGATGAATTAATTGA1140.15166431631322672No Hit
TTGTGTTTCTGGTTATACCTCTCATCCATGGTTTATGTTCATGATCTAATT1130.150333927573637No Hit
ATATTATGTGTTATCTGTCGCATTTGAGAATATTCAGTTCCACTGTATGGG1110.14767315009445758No Hit
GGAAAGATTACTGGAATACGGACTATTGAGCATTTGTAATTAGCTTTGAAC1080.14368198387568848No Hit
GTATATTAACAAAAACAGCCAACATTTTAATCCAACTGTATCTCTTAAGTG1070.14235159513609877No Hit
ATATACAAGTGAATAAAGCAGATGAAATCTTTGCTCTCATGGAATTTGCAT1070.14235159513609877No Hit
TCTACTACATCTTCTTTCTTCTCTTCTTTGTTATCAAACGTGATGTTTTCA1050.13969081765691935No Hit
CTTCTATCTTTCACTTCAAGTAAAATCTGAGCATTGGTTCTTTGGTGATTT1010.13436926269856053No Hit
ATATAAAGCAAACATTGGCATATCTGAAGGGAGAAATAGCAATACAGTAAT1010.13436926269856053No Hit
ATATTAAACCATCTGAACTTCGAGGTGTGAAATCTAATGGGATGATTTGCT990.1317084852193811No Hit
GAAGGAAGGAATGAAAGAAAAAAGACAAGAAGGAAAGAAAGAAAAGACAAA960.12771731900061198No Hit
ATCTGGAACTCTATTCAAATGCATGTTTGACTTTTTCTTCCTAGCAATCCA940.12505654152143256No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCTTC930.12372615278184286TruSeq Adapter, Index 12 (95% over 24bp)
CAATAAGTCATACACAAAAGATATGACAATTGTTTCTAATGCTTCTTGTAC930.12372615278184286No Hit
ATTGATAGACCATTAGCAAAATTAACCAAGAAAAGAAGAGAGAAAATCCAA920.12239576404225315No Hit
ATCCTAACTTTTTCAATGTCCTTGAGCAAAACACTTAAATATTCTGTGCCT910.12106537530266344No Hit
CAACCAAAACTTCTAACGGATTGAGTAAGGTGTAAACAATGATTTTTGGAA910.12106537530266344No Hit
GTATTCAAACAAAAAGCGTGTATATGAATGTTCATAGTAGCACTGTTCACA910.12106537530266344No Hit
GAACATCCTATCATCAAATACATCACTGAGTTCATTTTTAAGAAAGAAGCA900.11973498656307373No Hit
GATGTATATATCCCTTGCGAAACTTGTAAAGGAAATCGATATAATAGAGAG880.11707420908389432No Hit
ACATAAATGTCTAGAAAATGCTCTCCTTTCTAAAAATGTATTCCTCTACAG880.11707420908389432No Hit
ATCTGAATCTACTCTTTTTTTTTTTCTTAGTCTGGCTAACGGTTTGTCAAT870.11574382034430461No Hit
ACTTCAAGAAGAGTTAGATAGAGTACGTGATGAAGTATATGAAAATCTTCA870.11574382034430461No Hit
GTATAGTCAGTAAGGGGCTACTAATGATAAATTCCCTTGCGTTTGAAGAAT870.11574382034430461No Hit
TTACAATTTCATGCAGCTTTAAAATATCTATCGCTCTTAAGCTATTCGTAT840.11175265412553548No Hit
GTATAATATTGTTCCAACTGCATAAAACGAGACAAATTTAAATTACAGCAC820.10909187664635606No Hit
AATATGTACACCCATTGAAACAGCTTCTTCTATTTCGCTAAAGTGAATACC820.10909187664635606No Hit
TTATACACGTATTCTCAGATCAAAGATTTAGAACGAACAAAAGTTCATACT810.10776148790676637No Hit
ATAATAAAAGCATTATCACCCATTTTTCTCTTCCAGTCCTAAAACATGATG790.10510071042758695No Hit
GTTTAGTAACTGTGGGTAAGTTTCAAAAAACAAACGATAAGTCTTCGTTAT770.10243993294840753No Hit
ATCTATGTTTCATCAAGCTTTATCAAAGGTTAAAAACGCTCACCAATTTTA770.10243993294840753No Hit
GGTATTAATAGAACAGATAAAACAAACATGATCACCACAATTGATGCAGAA760.10110954420881782No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGAG206.991235E-445.019
CTTGTAC206.991235E-445.045
GGGGATT206.991235E-445.021
CGGAATT206.991235E-445.029
ATGGGAT206.991235E-445.037
GGCAGCG206.991235E-445.07
GTCTGTA206.991235E-445.037
CGAAACT206.991235E-445.018
TCCTGAT406.670234E-945.028
GAACTTC206.991235E-445.015
CTATAGT206.991235E-445.03
AACTTCG206.991235E-445.016
GAGTAGA351.1917837E-745.01
GGACTAT206.991235E-445.020
AACCAAC206.991235E-445.043
CCGGAGA206.991235E-445.020
CGTCTTC206.991235E-445.045
TTACTGG206.991235E-445.08
TACGGAC206.991235E-445.017
TAGTTAC206.991235E-445.06
Produced by FastQC (version 0.11.2)