FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_14.351000000438fb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_14.351000000438fb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182549
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC4170322.84482522500808TruSeq Adapter, Index 12 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT2027611.107154791316304No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCG23501.2873255947718147No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGTTC207.001689E-445.0208324
TGCCGTC42050.044.6216445
GCCGTCT301.1367332E-437.49680345
CGAAAGG250.002103356635.99693314
ATATCGG250.002103356635.99693317
TGACAGC250.002103356635.99693335
GACTCCT250.002103356635.99693325
GTGTACG352.8125552E-432.14011823
GACAGCT300.00513819329.99744442
CGTATGG300.00513819329.99744442
TACCACG300.00513819329.99744417
TAGGTGA300.00513819329.9974449
TATTGCC300.00513819329.99744441
AAGTCAC300.00513819329.99744417
ATGCCGT64850.028.89884844
TGTACGT406.149192E-428.12260224
TCTCGTA67900.027.86581238
CGTATGC68150.027.86262741
TCGTATG68100.027.85004840
CTCGTAT67900.027.83267839
Produced by FastQC (version 0.11.2)