FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_13.351000000438d1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_13.351000000438d1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211469
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC2370311.208735086466575TruSeq Adapter, Index 12 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT115955.483073169116986No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC15190.7183085936945841No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC12650.5981964259536859No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCG11150.5272640434295335No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA8620.40762475823879624No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA8470.40053151998638103No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA7090.33527372806416067No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA6250.2955515938506353No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC5270.2492091039348557No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA4840.228875154277932No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC4420.20901408717116932No Hit
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC4380.20712255697052526No Hit
AAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAG3740.17685807376022017No Hit
GTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCACGA3370.15936141940426257No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA3260.1541597113524914No Hit
GGTATCAACGCAGAGTACTTTTTTTTTCTGTCTCTTATACACATCTCCGAG3060.14470206034927105No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC3050.14422917779911004No Hit
AAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCC2870.13571729189621173No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2670.1262596408929914No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC2660.1257867583428304No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC2560.12105793284122023No Hit
AAAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG2490.11774775499009311No Hit
AAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCA2460.11632910733961005No Hit
GGTATCAACGCAGAGTACTTTTTTTCTGTCTCTTATACACATCTCCGAGCC2430.11491045968912701No Hit
AAAAAGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGCCCACG2400.11349181203864397No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2360.1116002818379999No Hit
GTATCAACGCAGAGTACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCA2280.10781722143671177No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTCTGTCTCTTATACACATCTCCG2130.10072398318429653No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC25100.044.10265745
GCCGTCT552.7266651E-936.81740645
ACCATCA250.002103447235.9992458
CGGGGAG250.002103447235.99924517
CACCATC250.002103447235.9992457
ATGCCGT36350.030.39138244
GTACACA300.005132461430.0064644
ATAGAAC300.005132461430.0064643
GGGGAGT300.005138402329.9993718
ATGTGAA300.005138402329.9993719
AATCTCG37850.029.48418836
ATCTCGT38050.029.44747537
TCTCGTA38000.029.42701338
GCAATCT37850.029.42474434
CGAGACT38200.029.39074123
ACGAGAC38300.029.3727522
CAATCTC38000.029.36780435
CACGAGA38500.029.33704621
CGTATGC37950.029.28792241
CTCGTAT38050.029.2700839
Produced by FastQC (version 0.11.2)