FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pa_11.3510000004389e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pa_11.3510000004389e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275023
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC57912.1056420735720285TruSeq Adapter, Index 12 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT11480.41741963399424775No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC9700.35269777436796196No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA5140.1868934598197242No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC4720.17162200979554437No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG4600.1672587383600644No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGT3660.13307977878213822No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC3640.1323525668762249No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA3560.12944371925257162No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC3430.124716841864135No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT2900.10544572635743192No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACT207.0130144E-445.014192
TGCCGTC7550.039.9318445
GCCTCGA301.13798975E-437.49818432
GTCCAGA250.00209919936.01791
ATGCCGT8650.034.593744
TATGCCG8900.033.62196443
CGTATGC8950.033.1827541
CTCGTAT8950.033.1827539
GTATGCC8950.033.1827542
CGAGACT8950.033.1827523
GCAATCT8950.033.1827534
CACGAGA9050.033.06469721
ACGAGAC9150.032.94922322
TCGTATG9050.032.8160940
AATCTCG9050.032.56748236
TCTCGTA9150.032.4574438
ATCTCGT9250.032.3497837
GACTACT9200.032.28104426
CCAGGCC352.8156373E-432.14129628
AGCCAGG352.8156373E-432.14129626
Produced by FastQC (version 0.11.2)