FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_95.35100000043718.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_95.35100000043718.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25633
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC300411.71926813092498No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGT1860.7256271212889635No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1050.40962821363086643No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCG1000.3901221082198728No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA850.3316037919868919No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT710.2769866968361097No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT690.26918425467171225No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA650.25357937034291733No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA640.2496781492607186No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT610.23797448601412244No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA600.23407326493192368No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA590.23017204384972495No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA560.2184683806031288No Hit
GAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.2184683806031288No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA530.2067647173565326No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.19896227519213516No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.1638512854523466No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA420.1638512854523466No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.1638512854523466No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA390.1521476222057504No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA380.14824640112355167No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA280.1092341903015644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3850.043.24675445
GCCCACG4100.041.15853518
CCACGAG4100.041.15853520
CACGAGA4100.041.15853521
CCCACGA4100.041.15853519
GGATAAT4100.041.15853532
ACGAGAC4100.041.15853522
ACCCTGG4100.041.15853527
GACCCTG4100.041.15853526
AGACCCT4100.041.15853525
GAGACCC4100.041.15853524
AATCTCG4050.041.1111136
ATCTCGT4050.041.1111137
GATAATC4050.041.1111133
ATAATCT4050.041.1111134
TCTCGTA4050.041.1111138
TAATCTC4050.041.1111135
ATGCCGT4050.041.1111144
CTCGTAT4050.041.1111139
CTGGATA4150.040.6626530
Produced by FastQC (version 0.11.2)