FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_93.351000000436e0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_93.351000000436e0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120884
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC56994.714436980907316No Hit
AGAGATGTATGAAAACTCTTGTGTAAGAAGTCTGTTATAATCGGATATTTT12961.0721021806028919No Hit
GATAGGACAGTAATAAGATATGACCTTTCTGAGTTTCTAGTTAACATGTTT10150.8396479269382218No Hit
ATACAAACACAGGAAGTACCAATACGGTATCATTGGATTTGAATAGAGCAG9580.7924952847357798No Hit
TGTTTGGTATTAGCATAACTATGTAGTAAAGAAACAGCTACCGATTGTACT8380.6932265643095861No Hit
GAATTGGTAAATGAGATTTCTGAAAGTTCTTTTGAATCAAGTATCTCTTTT8240.6816452135931967No Hit
TAACAGTACTTTGTAAAAGGACATGAGAGAAATTGGTAGGTGTTCTACATA7220.5972668012309321No Hit
GTATAAAATTCACTGGTACAGCAGATACACAAAAATGAGAAAGAAAATGGA6720.5559048343866848No Hit
AGGAACAATAAGATGGACAAGGTTACTTTTTCAACGTTTGATGCAAGTAAA6720.5559048343866848No Hit
TCCTAAACCAGATTACGAAATTTCAAAAGAAGATTGGTATGTGAGGAACAA6320.5228152609112868No Hit
TGTATGCAAGATGGGAAAAAGTAAGAGATTGAAAAACAAACCACAACCAAT6280.519506303563747No Hit
GATTTTAATGAAAGCCATATCTGATGTTTCAAATGGACAGAAAATATCCGA6090.5037887561629331No Hit
AACAATAAGCGTAAACATTTGCCATAGTTCTTTATCACTTTTTACAAGTTG6000.49634360213096856No Hit
ATAATGAACCTATTGCATTCTTTATTATGATTCCGGAGTTGAATCAGATTT5590.46242678931868564No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGT5370.4442275239072168No Hit
CAATACAATAAAAAACTGTGTGCATTTTATTTTGAACGTAAAGAAGCTTTG5060.41858310446378344No Hit
CCTTAGATTTAAAGTTTCTAAAAGAAAGATACCAGGACACATCTTTACAAG4780.3954204030310049No Hit
ATTTAATACAGTTTTCTCAGTGACTGATAGAACAAACAGAAAAGGAATCAG4680.38714800966215546No Hit
ATATATACAGTGAAGAACTGAATAATGCCAAGCAGGAATATATACTGACAC4610.38135733430396085No Hit
GTATAAAATTGTTGTTCTGAAATCTCTTCAACTTTCATTTACAATGCATCC4540.3755666589457662No Hit
ATCCATATAAACGCATGCTTATTGTTTATCGAGGTATAAACTAAAGGTATC4250.3515767181761027No Hit
GATGAATCCATATTCTCATCACTAAGATCATCCCATATGGGTTTTATTGAA4180.3457860428179081No Hit
ATACTAGAACAAAGATGATGATGAAACTGACAATGATGATTATGTCACAAA4130.3416498461334833No Hit
TCTCAAGATAAGAAACCATTGTCAGAATTTGTAACTAAAGAAACTGCAAAC4090.33834088878594354No Hit
TTCACAAACAGTGATAAATTCTTTACCCGCTTCGATTTCAACCTTGATAAA4000.33089573475397904No Hit
ACCATCTATATTGTCAAGTTTGTGGTCAAGCTTATCCTACATATGAACTCA3930.3251050593957844No Hit
GAATTCAAAAGAAACATCTGAGTTTTTAGAAAAGTAGCAGTTATGTAATCA3710.30690579398431556No Hit
ATATTCTTGCATATCATCCAGTGAAGGATTTAGCTCTTGTTATTTTACAGC3660.3027695972998908No Hit
ACCTAATTGTTGATCATACAATGTTGGGAACTGGATATTACGTGAAGTTAG3590.2969789219416962No Hit
GCATTTATGACAAAGATTTTGATGATGTTCAAACACATATTGAAGAGAGCA3490.2887065285728467No Hit
GTGCAGAAATGAAATTTTACCCTGAAATTTGAAATTGTTCATTCTATGGTG3460.28622481056219184No Hit
ACTCTATTCTCATAACAGAAGGTAGATTGCAAGGTTTATCATTTCGCCATT3350.2771251778564574No Hit
ATTCATATTCCTTTCTGTACAATGGCAGTGCATTGAATTCAATGCTCAGTC3310.27381622050891763No Hit
CGTTAACTGTTAGTGATTACAAGAGAAGGAGAGGTCTTCTCTGATTTATTT3310.27381622050891763No Hit
CATACATGTATGTATTCCACCAAAAACCTATCAGTGATTAATGGATTGTGA3300.2729889811720327No Hit
CAATATAATATTGATGTCAGAGCTAAAAGATGCATCAAAAGGGTATATGGT3160.2614076304556434No Hit
TTACTGCATACAGTAGATACGTTGAATAAGAACAATGATATCGATGGTATC3150.26058039111875847No Hit
ATATTGAAACATGGGAAGAGTTTTGTAAAGCAACAGAAAGATTATATCAAA3150.26058039111875847No Hit
GTACATCACTACTAACTTCTAACGTATACCGCTAACTTTTTTTACTTTCTG3130.2589259124449886No Hit
AGTTTTTATGATCCACTTAGCTTAATAAGCTCATGTTTCTTCTCATTTACA3070.2539624764236789No Hit
AGAAAGAACCTACTTAAACTACTTAGGAACCTACTTTACTTAGTACTAAGG2970.24569008305482942No Hit
GTATATTCCTAGATATCTTAGGGTTTTGGGTGCAATTGTAAATGGGACTGA2890.23907216835974981No Hit
TAATGGCTGAATAATATTTCACACAGTTCTATAGTTGTTGCTTCCATTTCA2880.2382449290228649No Hit
ATACTTTATTGTGATTTTTGAGTTGCATATTCCTGATGATTAGTGATACTG2730.22583633896959068No Hit
AAAAAAGACAGATAACTCAGAATGATATTTGGCGAAACTTTCACCTTTTGA2700.22335462095893582No Hit
ATATTATGTTCTGCTGTAGGGTTGTCTCCAATTTTTTTTTGCTTTACGTAC2690.2225273816220509No Hit
GATATACCAGAGTTATCTAGAAAGCTTTTAAAAGTATACTCAGACCACTCT2630.2175639456007412No Hit
GTCTAAAAGTAACACTGATTTAATGCAGTTTGCATAATTTACTAATCTTTT2600.21508222759008636No Hit
GTACGGGATTCTATTTGAATAAGCAATGTATTCTTCAGATCTTCGTCATTT2580.21342774891631647No Hit
GTCTTTTCCTTTGTTTTTGTTACTTGACACAGTTTTTTTTTTTGTTTCCGG2560.21177327024254655No Hit
GTTATATACACATCGATTTTTTTAGTTTTCCACAGAATGTACGCTTTCCTT2560.21177327024254655No Hit
GAATCAAATTGAAAAGAATCCAATCTAATCGAAAACATCGAATTGAATCGA2550.21094603090566164No Hit
GGATTAAGAATGCTTCAATCTCCACAAGATAAAAGACACATATCAGAAACG2510.20763707355812183No Hit
GTAACATTTTTTGTTGTCTCTTTTTAATTTGTCTCGAGTATAAACGACATT2510.20763707355812183No Hit
CCTTAAGGCTTACCTATCAGAATGTGAGGTATTTATTTGTTGAAAGATGTG2500.20680983422123686No Hit
CTTTTAAAGTACATCAAAGATAAATACGCAAACCCGGAAATTATGATCATG2500.20680983422123686No Hit
ATATCTTGCAATCACTTCAATCAGGTTCATATCTTGATACTCAAGTTGTAT2490.20598259488435194No Hit
GTTTTATTGTTGTGGATAGATTTTTAGTTTGCTTTAATACCAAGAAGGATG2490.20598259488435194No Hit
CTCGTATGATTGGTTTGAACAAAAAAACACATACAGAAAGAAGAACTCCTT2420.2001919195261573No Hit
CTTCAGTAACAGTTAATTTTCCCCTTTTCTCATTTTATTTAGCCACGGAAT2400.19853744085238742No Hit
TTTTATATGTGCACTCTTCAGAACAATAATGGCTGCCTCCTTTTCTTTTTT2390.19771020151550245No Hit
ATTGGTAGTAATTGCTTTTCATCCATTTTCTCAAAGAATTGGAAATAGGAT2350.19440124416796267No Hit
ACAGTAATTGCATTAAAGTAGCTGAATGCTTTTCTATCTACATTTTGACTG2320.19191952615730784No Hit
TATTTGAATTACTTGACTTACCAAACCAACAATTGTACATGGTTAAACTAA2210.18281989345157343No Hit
TGATTAATAAGCTGCTTTTTCAAACAAAATTACTACTTGCTCATATACTGT2190.1811654147778035No Hit
ATATTGGGAAGACGTTTTATTCGTTTTGGAAAACCAGACTCGCAAATGTTT2170.17951093610403362No Hit
ATTATGTGGAAGGAGAAAGAAATTTTCTTCTCTGGTCTTGTATGAATGCGA2150.1778564574302637No Hit
AATAAATCCTACATTGTAGTAACTTAATCCAAACCTTTGTAAAAACTTTGT2150.1778564574302637No Hit
ATGCTTTCACCATTAATTACACGATTGTATGCCTCTTTACCTAGTCCACCT2140.1770292180933788No Hit
TTGTTAAGCCGTTACTGTTCCATCTTCTGTAAATCTATCAGCAATATTGCC2130.17620197875649382No Hit
ATACTGATGATCCTGACCTTATTGATTCTAAAAGAGTTATTACCCCAGATG2120.1753747394196089No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2120.1753747394196089No Hit
CAAATACTAGAAAAACAGATGGTTATTAAAGCTTAGAAATTCCCCAAGAAT2110.17454750008272393No Hit
ACACCAAATGCAATGAATATTTTCCTCTTGGGAAAAGTAACAACAGATATG2080.17206578207206907No Hit
GTTTACCAAACATTTACTGTTTTCAATAGGGTATCAAATTCCAAACAATAA2050.16958406406141427No Hit
TTATTGATCTAAAGTCATCACTATAATGTCCATCATAAACTGTCTCTTATA2030.16792958538764435No Hit
ATATACAAACATGCATCTCTCTCTATTCTATACGACGAGTTCATCTTCAAG2000.16544786737698952No Hit
ACAACAAACTTACTTAGGAAACATCATTTTACTTGACATATCCATGCTACC1990.16462062804010455No Hit
AAATAAAACTGAGTATTTTGCTTTACTCCTAAATTCTCACTACAATTTCTG1990.16462062804010455No Hit
ATATATTCCAAGTGAGCATGGTTACAACCTGCTATAAATATGACATTACGT1970.16296614936633466No Hit
TTTCTATGCTTGTATTTGACTTATCTGGATGATGTTTATGTACTTTGTTTT1970.16296614936633466No Hit
ATCCAAAACAACGAAATTTTAATTTGAGGTGAGTCTATTTCTCTGCAATTT1970.16296614936633466No Hit
GAGTAGCAATATTCAACAAAATTACTTTGTTGGTGAATAAAGCAAATAGCA1950.16131167069256477No Hit
TCTTCTGATTATGTCTTAAACTCAATGTGAGACTCTTTCGTATTAATGGTT1940.16048443135567983No Hit
ATATCAATGTTTCATCATATTTGTGTATCTTCCTGCCATAATGCAATGACA1880.15552099533437014No Hit
ATGTAAAAGGAGAAAAAAACAGATCTTAGGAAGAAATGACTCTACAAGAAA1870.1546937559974852No Hit
AAAATACTCAAATGGTAGTAAACGTGTTATGTTTGTTGATGCTGTAAAAAA1870.1546937559974852No Hit
ATATGGTCTATCTTGTTAAGTATCCCATGTTCACAGAAAATGTATATTTTG1870.1546937559974852No Hit
TAATCTCCTACTGGGGATACATCTTCTATGCTGAACAAGGCTTAAATAAGA1870.1546937559974852No Hit
TATTGATGGTTTTCTTTGTTGTGCAAAAGTTTTCAATTTTGATGTAGTCTC1840.15221203798683036No Hit
GTTATAATAACAGATTCGGACTCTATTACGTCGATTTCAAAAATAACCTCA1800.14890308063929056No Hit
ATATGGTTCATTTTTATTGAGAATGTAACAGAAACACAGAAGGGAAACTAT1800.14890308063929056No Hit
TTGTAAAGTTGAAAAATCAGAACCTCAATGGGATGATGTAACTTCCCCAAG1800.14890308063929056No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCG1790.14807584130240561No Hit
TAACAATTCATTCAAGACTTTCTTAAAAAACATGTTGCCACATGAACTCAG1780.14724860196552064No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1760.14559412329175075No Hit
TTATTATACGTAAATGCCTGTTTGGGATCCAAGGCTAATGATTTATCAAAA1750.14476688395486584No Hit
AAATAAGACATCCAGATGGCTAACAGACACATGAAAAAATGCTCAATACCA1740.14393964461798087No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1710.14145792660732603No Hit
ATGTACTTTAGAAAAGGATAAAGGGTAGGTAAAAGATATCTAAGTCTCTCT1710.14145792660732603No Hit
AAAGTATACAGCACAACATGTTAAACACCTACTTAATAGCTAGCACAAACT1700.1406306872704411No Hit
AGGTATCAGTTTGTAGGTAAATATTTAAAGTCACCACATTTGGCAGAGAAC1700.1406306872704411No Hit
GTAGTACTACCTACTAGTAGGTAGTACTACCTAGTAGTAGGTAGTACTACT1700.1406306872704411No Hit
ATATCAATTAAGCTCATTGGATTCCAGCTAATTAAGTTACAAAGATAAGGG1700.1406306872704411No Hit
AAGGAGAAATATCTTCTGTTCAAGTGAATTCTGAAAATGTTTCGAATGTAG1680.1389762085966712No Hit
GAAAGTAGTGTGTATGATGTAGTAGTGCTTCTAAAATTGATTTCGTGCTTA1660.13732172992290131No Hit
AACTATAAAGGCAATAAAAGATGACAACTGAAAACAACGAAAAACTTTCTT1650.13649449058601634No Hit
GTTCTATAGAATGGAATTTTCTTCTTCTCCATTGTGCTACTGAAGAAAGGA1650.13649449058601634No Hit
ATTCATTGGTGATTCTATTCAATTCAATTCTTTGATTCAATTCCATTCCAT1630.13484001191224645No Hit
TTTCAGTATTTGTCTGCAGTTATTTTTATGCGTTCAAATAAGTGTACAGTC1630.13484001191224645No Hit
AAAATATGGTGTGAATATATCAGTGATTGCTAAAGGTTGGGGAGTTTGTGA1610.13318553323847657No Hit
GTATATATAGCTTCAGTATCGCAAACAAATTTGTTGTTTCCATCTGATCGA1610.13318553323847657No Hit
GTAATATTCCATTGTATACACAAACCACATCTTCTTTATCCATTCATCTTT1590.13153105456470668No Hit
ATGTAATCCTCATCAAATGGAATCAAAAATAACCATCATCAATTGGTATTG1580.1307038152278217No Hit
TTCAACATGTACATGACATTCCTCCTAAGACGATTCATCCAAAAAACACAA1580.1307038152278217No Hit
ACCTATTCTTCTCAAACTATTCCAAAAAAAATCGAAGAGGAAAGAATGGTT1580.1307038152278217No Hit
ACTACTACTCTAGTAGTAGTAGACTACTAGTAGTAGTAGTCTACTACTACT1540.12739485788028193No Hit
GCTAGACATAGATCATGTCTTTTGGATAGTATGGATTGAAAACCGAAAATA1540.12739485788028193No Hit
GTACAACATCAGCAAATCAATCAACATGATACACCACATTAACAACATGAA1510.12491313986962707No Hit
ATGTAAAGTCTTTCAAATGAAGTCAATTTTTACGTCACTACTTATATTCCT1490.12325866119585718No Hit
ATTTTATACCGCAATATGGGTTGGAAATAAAGCTATTTAGCTGATTTTCAT1490.12325866119585718No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1470.1216041825220873No Hit
ATGATGCCATTATCTTTGAAGCTGAAATGGTTAAGGTCTCTGTGACAAACA1460.12077694318520234No Hit
GTACATAACAAAATGAAGGCAGAAATAAAGATGCTCTTTGAAACCAACAAG1460.12077694318520234No Hit
GTATTAGTTAGGGGAATATGGAGAATGGTAATTGTATTGACTTATGACTGT1460.12077694318520234No Hit
AAATATGGGTCATGTTTTTGAATGAATGAGCAAAATGTTGAGTTTAGCTGA1450.11994970384831741No Hit
AAGATAAAAGCACGGACTTCCATCTAGTTACTACACTCATTTTTCTGTTTA1430.1182952251745475No Hit
TTTTATTATGTGCCAAGTCCTAATGGAAGGATTGGGGATACAATGTAGAAG1420.11746798583766255No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1410.11664074650077762No Hit
GGTAAAAGTCTTCAGTAATTTTTCTTCCCCAATATTTCTTTCATTAGTCAT1400.11581350716389266No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA1380.11415902849012277No Hit
CATCATTATGGTATAGCAGATAAATGTTGATACAACTTTACAAGGAATTCT1380.11415902849012277No Hit
GTTTAAACGTTATAGTATGTTCTTGCAGTGTATTGGCATTCAAATGCATTT1370.11333178915323781No Hit
CAGGAAGATCATATTCAAATTCTGGATTGTGATGATGGTACAAAAAAGAAC1370.11333178915323781No Hit
GTTTGATTTCTTCTTTCATGATTCATCCTTTAGCATAAGAAGGAACAAATG1350.11167731047946791No Hit
GAATAAGATTGTTGCCGCTACTTAAATCTTTAGTTTTGACTCATCTAAACG1350.11167731047946791No Hit
ATATATAACAGCCCTCTGATTGAAAAAGAACTCTTCCTGTTGCAGCTCTTT1350.11167731047946791No Hit
GTTCACATTTATTTACAAACGGCGAACAAGAACATTAAAATCGGAAAAGAA1340.11085007114258298No Hit
ATATCTTCAAGTTCAATCATTTTCTGATCTTCATCCATTGTTACCTTACTG1340.11085007114258298No Hit
TACATGTGTACTCTGTAAGGTTTAGTTGTTTTAAGTAAAACGAAAAATAAA1330.11002283180569802No Hit
TAGTAGTGATCTACAATCATATCAATAAGCGCATAGCATAAATACTGTCTC1330.11002283180569802No Hit
TTATATGGCTATGACATCACTAGATTATGGGAATTTTTCAGCTCCATTATG1320.10919559246881307No Hit
GAAATAAGAGAGGACAGAAACACATGGAAAAACATTCCATGCTCATGGGTA1320.10919559246881307No Hit
ACCATCATCAGTAAAACTTCATTCTTCCTGTTTCACAAGATGTTGTTCTCG1310.10836835313192814No Hit
GTATTATGTACTGCACATAAAATGTCAAAGTACCCTAAGTATGTATTCTGT1310.10836835313192814No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA1300.10754111379504318No Hit
GTATAATATGCTTTTCAAAAACACATTTGTTCCTTCTTATGCTAAAGGATG1300.10754111379504318No Hit
CTGTTGATTCACATGACATCTTGAAAGAATATGAAAGGCATTTTGCTGAAT1290.10671387445815823No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1290.10671387445815823No Hit
TTCCAATACACTACGTATTGATTTTTCAGTTTAACTGCTTGAAGGCAAACT1280.10588663512127328No Hit
CAAGGATAAAAACACATATCGTTCTTCAAATTCTAGTGATGCAATTTGAAC1280.10588663512127328No Hit
GATTATAACAGGAAATACTATCTTCAGAGGCATCTTCTAGCTATGAACGAA1270.10505939578438835No Hit
GTTATTTATAAATGCTTACATGTGTGGCAAAAATAGTTGATATCCAGAAGA1270.10505939578438835No Hit
TTTTTACTTTGGTTATTTTGTAGTTCTTCACTACTTTTTTCCATTTCCTGA1270.10505939578438835No Hit
TATCAGTACCGTAAAAAATGTATTAGACCAGTTGAATTAGTAGATAGTGCT1260.10423215644750339No Hit
GTCACTATCTTATCTGGAAGACTGAATCATTTTAAGAATTGGATACTGTTA1250.10340491711061843No Hit
CTATATTCTCCTCTATTTTGATAGTTTCCTATCTCACATTTAGGCCTTTCA1250.10340491711061843No Hit
GAGTATAGCACTTTAAAAACATACAACATGACTCTCATATATCCAACACAA1240.1025776777737335No Hit
ATACCGGGTTATATCTTGGTTATCAGCGTTATTTTGGTTTTAAAGGAAAAT1240.1025776777737335No Hit
GTACAAACATTTTAGAAAACTGGTGGTTTCTTTTAAAGGTAAATACACACT1230.10175043843684854No Hit
GTTAGAGAGCTATAGTTTGTAAGGCCTCATATTAAATTAATCAGGGATCTT1220.10092319909996361No Hit
TTCTTCTAGTTATTCCAATAATGTTGATCTTTTAGTTAGCTCCAAGGACAT1220.10092319909996361No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA1210.10009595976307865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGAT351.1979864E-745.0091130
GAGGAAC351.1979864E-745.0091143
GCAAGGT351.1979864E-745.0091129
CTACTCT253.8654187E-545.009115
CCTAGTC502.1827873E-1145.0091140
ACCGCTA351.1979864E-745.0091128
GATCATC502.1827873E-1145.0091126
GTCTAAA253.8654187E-545.009111
CCGCTTC351.1979864E-745.0091127
AGGAACC253.8654187E-545.0091125
TCAGGGA253.8654187E-545.0091141
GCTTCGA351.1979864E-745.0091129
GTATGGA253.8654187E-545.0091129
CACTACA253.8654187E-545.0091138
CGCTAAC351.1979864E-745.0091130
AAACCGA253.8654187E-545.0091140
GGAACCT253.8654187E-545.0091126
ACCAGAG253.8654187E-545.009116
TACTCTA253.8654187E-545.009116
GCTAGCA253.8654187E-545.0091139
Produced by FastQC (version 0.11.2)