FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_92.351000000436c6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_92.351000000436c6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58509
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC627010.716300056401579No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGT3430.5862345963868807No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1730.29568100634090483No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1550.2649165085713309No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1550.2649165085713309No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1470.2512433984515203No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCG1380.23586114956673332No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1280.21876976191697006No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1230.21022406809208838No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1200.2050966517971594No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA1170.1999692355022304No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1160.19826009673725412No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA1130.1931326804423251No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA1070.18287784785246713No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA1010.17262301526260918No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA990.1692047377326565No Hit
GAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA950.16236818267275122No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA920.15724076637782222No Hit
GTATTCTGTTTATGCCCGATTATTATCCATGCCGGACCGCTCGACTAGTGA820.1401493787280589No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA790.13502196243312994No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA660.1128031584884377No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA630.1076757421935087No Hit
GAGCTGTGATTGCTTGTAAATCCACGGTTTCAGGTTCTCTTTCACTCCCCT630.1076757421935087No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA620.10596660342853237No Hit
AAATCCGGGAGTGAGGCTGCGAGAGCTAACTTCCGTAGCCGAGAGGGAAAC610.10425746466355604No Hit
TTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT590.10083918713360338No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAC253.826456E-545.037641
ACCATGC206.9791154E-444.9991468
TGCTGAC206.9791154E-444.99914636
ATAACGC253.8458675E-544.9991463
ATGCTGA206.9791154E-444.99914635
GCTGACG206.9791154E-444.99914637
AATGCTG206.9791154E-444.99914634
AGTTACA206.9791154E-444.99914639
TGCCGTC6450.042.90616245
GTATGCC6700.041.30518742
GCCCACG6700.041.30518718
TATGCCG6700.041.30518743
CGTATGC6700.041.30518741
TCGTATG6700.041.30518740
CTCGTAT6700.041.30518739
CCACGAG6650.041.27741220
CCCACGA6650.041.27741219
GACCCTG6600.041.24921426
ATAATCT6550.041.22059234
AGACCCT6550.041.22059225
Produced by FastQC (version 0.11.2)