FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_90.35100000043676.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_90.35100000043676.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22858
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC306013.386997987575466No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGT2561.1199580015749409No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1590.6955989150406859No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1380.6037273602239916No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1290.5643538367311226No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1210.5293551491819056No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA1090.4768571178580803No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1080.4724822819144282No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1060.46373261002712396No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA1020.4462332662525156No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCG980.42873392247790704No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT970.42435908653425497No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA940.41123457870329866No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT870.3806107270977338No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA740.3237378598302564No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA740.3237378598302564No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.3062385160556479No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA640.2799895003937352No Hit
GAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA610.2668649925627789No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA600.2624901566191268No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA490.21436696123895352No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA480.20999212529530142No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA450.19686761746434506No Hit
GAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.192492781520693No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.17936827368973662No Hit
GTATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA380.16624376585878028No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA350.15311925802782395No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA320.1399947501968676No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAA300.1312450783095634No Hit
CTTCTCGTGCCGTTTCTGCTCTGCCAGGCCCTAGTGCGCTGCTCCAGCCCT290.12687024236591127No Hit
GGTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAA280.12249540642225917No Hit
TATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA260.11374573453495494No Hit
TTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT260.11374573453495494No Hit
GAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.10937089859130282No Hit
GTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA250.10937089859130282No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT253.7717044E-545.045
TGCCGTC3500.041.78571345
ATGCCGT3750.039.044
GTATGCC3800.038.48684342
AATCTCG3800.038.48684336
ATCTCGT3800.038.48684337
TCTCGTA3800.038.48684338
ACGAGAC3800.038.48684322
CGTATGC3800.038.48684341
TCGTATG3800.038.48684340
CGAGACC3800.038.48684323
GATAATC3750.038.433
ATAATCT3750.038.434
GGATAAT3750.038.432
CTGGATA3750.038.430
TAATCTC3750.038.435
ACCCTGG3750.038.427
CCCTGGA3750.038.428
TGGATAA3750.038.431
GACCCTG3750.038.426
Produced by FastQC (version 0.11.2)