FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_85.351000000435e1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_85.351000000435e1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96708
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC3510836.30309798568888No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT1522915.74740455805104No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCG69767.213467344997311No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC5420.5604500144765686No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA2900.2998717789634777No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA2660.2750548041527071No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTAT2530.2616122761302064No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC2340.24196550440501305No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA1550.16027629565289325No Hit
GTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCACGA1470.15200397071596972No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCC1470.15200397071596972No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGC1450.14993588948173886No Hit
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT1290.1333912396078918RNA PCR Primer, Index 44 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACT253.864535E-544.99948523
GAGACTA253.864535E-544.99948524
TGCCGTC36850.044.26679245
AGACTAC250.002097718235.99958825
GACTACA250.002097718235.99958826
ATGCCGT51700.031.5518644
ACTACAT300.00512453729.99965727
ACTACAA300.00512453729.99965721
AAAAAGT2750.029.4694421
AAAAGTA3000.028.499672
CGGGGTA406.1254733E-428.124686
TATGCCG60050.027.1645543
GTATGCC60300.026.97730442
CGTATGC60600.026.8437541
TCGTATG60450.026.8359240
TCTCGTA60500.026.77655438
TATCTCG60450.026.76147836
CTCGTAT60600.026.73236539
ATCTCGT60600.026.69523837
ATATCTC60400.026.63462835
Produced by FastQC (version 0.11.2)