H23LNBCXX_l01n01_bir_1228_pcontrolb_84.351000000435ba.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_1228_pcontrolb_84.351000000435ba.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	98441
Sequences flagged as poor quality	0
Sequence length	51
%GC	37

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC	10904	11.076685527371726	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT	1263	1.2830020011986876	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCG	719	0.7303867291067746	No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	263	0.2671651039709064	No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	212	0.21535742221228962	No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	164	0.16659725114535612	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	161	0.16354974045367277	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	158	0.1605022297619894	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	148	0.1503438607897116	No Hit
CAATAGAGAAATTACTGCAATACCTAATTTTATTGACGTTTCGCGCTTTAG	146	0.14831218699525606	No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	139	0.14120132871466157	No Hit
GAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	139	0.14120132871466157	No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	137	0.139169654920206	No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	136	0.13815381802297824	No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	136	0.13815381802297824	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	134	0.13612214422852265	No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	129	0.13104295974238378	No Hit
TATTAATGCTGGGTATCATGATGCTTTTTTTATCAGCAGCTGAAAAACTAA	125	0.12697961215347264	No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	116	0.1178370800784226	No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC	109	0.11072622179782814	No Hit
TTCTTAGACACTTATATTTGGGTTGTCTCTTATTTAGAACATGGATGCTGA	105	0.10666287420891701	No Hit
CTTTAGAACTGTGAAAGTAGTAAGAGTATTAGGCTGACTTCTATAATCACT	102	0.10361536351723367	No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA	101	0.1025995266200059	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCTGG	20	6.9847115E-4	45.02237	1
CTATGGT	25	3.8650294E-5	44.999493	6
ATCTATG	25	3.8650294E-5	44.999493	4
TCCTGAG	20	7.002239E-4	44.999493	43
GACGTTT	20	7.002239E-4	44.999493	35
ATAGCCG	20	7.002239E-4	44.999493	32
AGGGTAG	25	3.8650294E-5	44.999493	34
TAGCCGT	20	7.002239E-4	44.999493	33
TTGAGTG	20	7.002239E-4	44.999493	9
TACATCC	25	3.8650294E-5	44.999493	4
TACATAG	20	7.002239E-4	44.999493	3
TATTGAC	20	7.002239E-4	44.999493	31
AATGATC	25	3.8650294E-5	44.999493	2
AGGCATC	20	7.002239E-4	44.999493	12
TTGACGT	20	7.002239E-4	44.999493	33
CTTCTTA	20	7.002239E-4	44.999493	2
TATAGCC	20	7.002239E-4	44.999493	31
TAAGGGT	25	3.8650294E-5	44.999493	32
ACCCATC	20	7.002239E-4	44.999493	37
GGCTCAG	20	7.002239E-4	44.999493	9

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content