FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_83.35100000043591.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_83.35100000043591.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146057
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC127968.760963185605618No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT22291.526116516154652No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCG9200.6298910699247554No Hit
ATAGTCTTTTCAATGTCTTCCATAGATGAGTTTTCGTTAGCATTTACTTTT3010.2060839261384254No Hit
CATGTATATGTGAACATTGATCTCTTAAAGTTTCCTTTCTTGTGTGTTCAT2910.19923728407402588No Hit
TTTGTGTTCTTACTTTGTTTATCCATTCAAGATGAGTTATGATTGAGGATG2860.19581396304182613No Hit
ATTTTGTATAACTGACCTTAGTGAGATCAAACTTACTTCATGGGATATAAG2370.1622654169262685No Hit
GATGAGAATTGATTTCATTGCTTTTCTCACACTTAGTTAAGAAGGCAATGA2300.15747276748118885No Hit
TAATATCGGATGATGAATAAAATCCATATGTGCACGAATTTGATGTGTTCT2290.1567881032747489No Hit
TACATACTCTTTCTTTAATTGCTCTGTTTCTTCTTCTCCAGGATCCATACT2170.1485721327974695No Hit
GTATTCACTCTGCTATTATCAATTGTTAGGAATGGGTTATAATTCAGGGAT2160.14788746859102953No Hit
GAAATATTCAAAGTCGAAGATTTACATTCCTTTCGTATCACAATTAACCTT2080.14241015493950993No Hit
ATGAAGATATACCAGGGAAATGCTAACTAAAAGAAAGCAGAAATGTCAATA2070.14172549073306998No Hit
GATAATAGTTAGGTTCCCTGATCTAAGAGAAACAATTTGAGTGTTGAGGAT2070.14172549073306998No Hit
ATGATAATGGTGGTGATGATGGTGGTGATATGATGATAGTGATGATGAGAA2030.13898683390731018No Hit
TATTTACAAGGAGGCTGACTGTAGCATACTCTTAACTATCCATTTAGTCCA1970.13487884866867045No Hit
GAATGACTACTGGGTACATAAAGAAATGAAGGCAGAAATAAAGACATTCTT1950.13350952025579055No Hit
GTTATCTACATAGCGTCTGATATTGAGGGTATATTCATTTTCAGCTATGGT1940.1328248560493506No Hit
TACTTATACGCAATGAAGTACTATTCAACCACGAGATTCTTTTGCAACAAG1940.1328248560493506No Hit
ATGGAATGGTATGGAATGGAATGGAATGGTACGGAATAGAATGGAAAGTAA1930.13214019184291065No Hit
TATTTGTTCTGTGTATAATCTCAAAACAAGGGAAGGAAATGTGAGAGTTTC1920.1314555276364707No Hit
AAAGAAAGGAAAAAAAGAAAAAACAAAACAACAGCAATAAAACAACAAAAA1880.1287168708107109No Hit
GTTTTGTTTCTGAAATTCCACATATGAGTGAGATCATATAATTGTCTTTCT1870.12803220660427092No Hit
GTAACAACACTAAGTGCCATATACATGCATCTTGGCAATGCTTATCAATAT1850.12666287819139102No Hit
AAGTAAAGTGTCGATTAATGACATGATGTTAGCAATGTATTCTGTCTCTTA1800.12323955715919127No Hit
AATTTGATTACACTTTACAGTTACGAAGTCTTGAAACTTTGATTCGCAAAT1770.12118556453987142No Hit
GTTTAATTTTCCTACTTTTCTTGTGAAAGATTGCATATCATAAATGCTCAT1750.11981623612699152No Hit
AAGCAATGGTTATGTTACGTACTAAATTATACGAAGAGCAAGTACGTAAAC1720.11776224350767166No Hit
CTACTACTTACTCATTTCTACTTAACATTCTAACGATGGCAAACCAAATCT1710.11707757930123171No Hit
TAGTAGTACTACACCGATTCAAAAGTATTTCAAATCGAAACACAAAACATC1710.11707757930123171No Hit
ATTTTTAAGGCTGAATAATATCCCATTGTACGTACATAACACCTGTCTCTT1700.11639291509479176No Hit
CTACAACAACAACTGATATGAGATTGGATGTACACAAACAATGTATAAGGA1690.11570825088835181No Hit
GATAAGTACAGTGAAGTTCGTTTTTTGTTTTGTTTTGTTTTGTTTATGTTC1670.11433892247547191No Hit
TCATAAGGAGGACAGTAATCAACGTATATGAGTTTGGGACTTAGAAATCAA1670.11433892247547191No Hit
ATCTTAATGTTCTTTGACAGGTACATCCAGAGAATGGAATACTATTCCTGT1670.11433892247547191No Hit
GTTAATGATCATAACATCTCACTCTCAGAAGAGTTACTTACAATTTTGGTT1640.11228492985615206No Hit
CAATATACATTTCTCTTCAAGTTCTTGTAACACAAATGTAGCTTTGTTCAA1610.11023093723683221No Hit
TATTCATACAAAGAAACATTAGACAGCGATAAAAAGGAACAAACTCCAAAA1600.10954627303039226No Hit
GATCATGATAATAGTACCCAGATCATAGGGTTATGAAGGTTAAATTAGCTA1590.10886160882395228No Hit
GAATCATCAAATGGAATTGAATAGAATCATCGAATGAAATCGAATGGAATA1580.10817694461751233No Hit
GTAATAGACATATGCTTGACTTACACTTTTTTTGTTAATTTGCCTGTCTCT1580.10817694461751233No Hit
ACATAGTACTGTGAAGAAAAAAAGCAAAACAGGGAAAGTACTGCTATTAAG1570.10749228041107238No Hit
CTATTAAAAGACAAAAATCCAACTGAGTAAACTTACAGATCAAATTGGTGT1570.10749228041107238No Hit
TTTTCATATCATGCCAGTAAATCTTACCCATTAACTGTCTAGCATATTTTG1570.10749228041107238No Hit
GAATAAATTTTGTGTCTGTTTCATAGTTGCTACTACCATCTTTACTGGTTA1550.10612295199819248No Hit
GAATGAACTGGAAGAAAACGAACAAACAATAGGAATTTTCTCTCCTTTGCT1540.10543828779175253No Hit
CATAAAGTGAGTCAATGCAGATATAAGAAGCATTTGTAATGTGAGAAGCAA1540.10543828779175253No Hit
GTTTGATGTTGATTCCATTAGATTCCATTCGATGATAATTCCATTCGATTC1530.10475362358531258No Hit
CTGTTACCATTTTGGTAGTATATCAAAATGCACTAGTTCATCATTTCAGTA1530.10475362358531258No Hit
ATATCTTCATAGACCAGCTGTAAGTGATGGTTGAAGTTTGAAGAAGTAGTT1520.10406895937887263No Hit
AAGGAATCCTCATTGAATGGAAATGAAAGGGGTCATCATCTAATGGAATCA1520.10406895937887263No Hit
TATCACTGTTTATCCTGTTTTGTTTCAGCAAGTACTTATGAATCTCATTAG1510.10338429517243268No Hit
GATCGAGTAATGTAGTGACATATTAACAGAGTGAAGAAGTAACCAAGTAAC1510.10338429517243268No Hit
CTATAGAGCTAAGTATGATGCTAAGTTTAAGCCCTGGTATTTAGATAAAGG1510.10338429517243268No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGCTG253.8742197E-544.9996637
TCGTTCC253.8742197E-544.9996621
CAACGGC253.8742197E-544.9996624
TGGGTTA253.8742197E-544.9996633
GACGTAG253.8742197E-544.9996617
GACAACT253.8742197E-544.9996626
ATAGCGA253.8742197E-544.9996629
ATTCGTC253.8742197E-544.9996638
CGTCTAC253.8742197E-544.9996641
ACTTACC253.8742197E-544.999668
AACGGCT253.8742197E-544.9996625
AAGCCCT253.8742197E-544.9996629
ACGGCTA253.8742197E-544.9996626
CGTAGGA253.8742197E-544.9996619
GCCCTGG253.8742197E-544.9996631
ACCAGGG253.8742197E-544.9996611
TGTTAGG253.8742197E-544.9996624
ACGTAGG253.8742197E-544.9996618
CACAACC253.8742197E-544.9996641
CCTATCC253.8742197E-544.9996614
Produced by FastQC (version 0.11.2)