FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_82.35100000043577.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_82.35100000043577.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88093
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC1857721.087941153099564No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT81719.275424835117432No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCG27593.13191740546922No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC5580.6334214977353478No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC2210.2508712383503797No Hit
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT1950.22135697501504092RNA PCR Primer, Index 44 (95% over 22bp)
AAAAAGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGCCCACG1760.1997888595007549No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA1280.14530098872782174No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA1260.14303066077894952No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCC1170.13281418500902456No Hit
TGAACCTGATAATGGAATTGATACGGCTTATTATTCTGGACTTAGATTTGT1060.12032738129022737No Hit
GTATAATCCCTGATGAATAAGTGTTCTACTATGTTTCCGTTCCTGTGTTAT920.10443508564812187No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCG890.10102959372481354No Hit
AAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCA890.10102959372481354No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGATG206.998685E-444.9988636
TAACGAT206.998685E-444.9988635
GGTTTGG206.998685E-444.99886318
TCTATGA206.998685E-444.99886312
ACGATGA206.998685E-444.9988637
GCCAATA206.998685E-444.99886336
CGATGAA206.998685E-444.9988638
TGCCGTC20800.043.8089945
CAATGCC250.002096840635.99909237
TAATCTT250.002096840635.9990924
AAAGGCT250.002096840635.9990926
GCCATCT250.002096840635.99909226
CGTTTTA250.002096840635.99909224
ATGCCGT28300.032.19883344
TCTTGTC352.7998292E-432.1420488
TAATAAT352.7998292E-432.1420482
GAATATA352.7998292E-432.14204843
TTGAACA352.7998292E-432.14204838
ATTTAGC352.7998292E-432.14204821
GTATTTA300.005094002530.0333521
Produced by FastQC (version 0.11.2)