FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_79.3510000004351a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_79.3510000004351a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65598
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC38915.931583279978049No Hit
ATTAGAAACTGAACGAAAAGTAGAACGTCAGTTATTCCAAGTTCATTGTGA6210.9466752035123023No Hit
ATTTAGATGATCCTTCTAAAAAGAAAAGCAAAAATGAAATTCCTGAAACGA4260.6494100429891155No Hit
GTTGTATGCATGTACAATAAAGTGAACAGAGATATAAGCGCAATAACCTGT4050.6173968718558492No Hit
TATCTGTACTATAGAACTGAGATTTGAATGCAAGAATGCACTCCATTTTTT3750.5716637702368975No Hit
AGATAAACGAGTATCGTAAAATAAATACCTGCCTAACAATTGTAAATCCTC3520.5366017256623677No Hit
CTATAGCTATGATCTAAGCCGATATACTTATCACTAACTATCCTTCCATCT3510.535077288941736No Hit
GATGTAGACAGCATAGCTTACAATAGTTTCGATGAAATAGATAGTACGTAT3160.4817220037196256No Hit
ATTACAACCTATTTGGGCGATTATTATGTTGCACTTTCTTCTGTGTTTAAC2880.4390377755419373No Hit
TGATTCATCTTTTAATCATAACTTTCCTGGGCATATTTTTTTGCCTGCAAT2830.43141559193877865No Hit
ATGTAGCCTACTCAAACATGTAACTGATAAGGAGTCTTATGTTTCTATGAT2810.42836671849751523No Hit
GTATTGATCAATACTGGAAATATGAGCAAGTATCATGATTTTGTTTTTTCA2730.4161712247324614No Hit
GTACAGGTGTTATATCAAAAGATCCCTCTGTTAAAACAAGCAAAGATGATT2590.3948291106436172No Hit
GATCTACATAGAATGCTAAAGATGATGTCAGACTTCGACAGAAAATTCTTT2530.3856824903198268No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT2520.38415805359919514No Hit
TGTAAATACTCACAAATTGAACAAGCGTTTTTGCAGAGTTGATTCTTTTAT2500.38110918015793166No Hit
AGACTGTATATACTGAATAGCTTTAGCAGGGTTATGAAAAAGTTGATCATT2470.3765358699960365No Hit
GGATATACTGCATGAGCATTTAACTTTTTAACAATCTCATTGACATCAAAG2310.35214488246592884No Hit
GTCATTAACAATTTGAATGCTTAGATACTCTTCGGTCAGTTCAACAGGAAT2300.3506204457452971No Hit
ATTGCTAGTAATACTGGTGAAACCCGTAGTTGATTGTAAAGGTTGAGATAG2300.3506204457452971No Hit
TCCATAATCTTCGGTAAGAGATTCATGAATGTTCTTCATGATAACTGAAGT2170.33080276837708467No Hit
GTTTTTTCTCATTTGCAGTAAACTTGTTGAATAGTTCTTTACGATAAACCT2150.32775389493582124No Hit
ACTTTTCTACGATTATCGAATACAAATCGTTAGGATTAAACGGTTTTGTAA2130.32470502149455777No Hit
ATTGTGAACCATTAACATTCCTCATCATACTGTTTCATTTCTTTCCCCCTT2130.32470502149455777No Hit
ATACGAGCATTTGATATTTGATGATTTAGCGCATCAAAGTATATTACGCTA2110.3216561480532943No Hit
CTATTTATTGGAATGTCAATTTAGCTTTGAGTACGAAATCTATCATCAAAT2080.3170828378913991No Hit
ATTAAAAGACATTCTTCATACAAGTCAGTTTTTAGCAGTTGTACAAATAGT2070.3155584011707674No Hit
TCATAATCCCCACTATGACATCACAATCCACAATATGACATCACAACTTTC2060.31403396445013565No Hit
ATCAAGAGTAGTGTTTATGTTGCTTAAGGCTTGGATAGTTACAATTTGTGT2040.3109850910088722No Hit
TTCCCAAACTTTTGATGATATACGCAGGAAATTAACGGAGATAGATATCAA2040.3109850910088722No Hit
CAATACAAAAACTCGTGTTTAGCAAATGGTTTTCTGACTTCCAACTTTATA2020.3079362175676088No Hit
GTATGCTTGAATTTCAACTTGTCCATCTTCATCTTCTTCTGCATTAACTGG2020.3079362175676088No Hit
GCTTACTAATGGCACAAACAAATTTTGATGTTAAAGAGCATAATGCAAAAG1920.2926918503612915No Hit
GGTAACTACTATTAGTTTGTTCCGCGGGAAAGAAACAGTTACATTTTTGAG1870.28506966675813283No Hit
ACTTACTATCATGAAAGATAGTAGATTAGGAACCTATGGCGTAATTGGCCT1830.278971919875606No Hit
ATGTAGTTTCAAAACGTGGAGATTCTGTTGTAGTAGTAGGTATTGTTGTTG1780.27134973627244735No Hit
ATGTAGACGATAGGGTTGGTAGTATCAAAGAAGGTAAAGACGCAGATTTAG1690.2576298057867618No Hit
ATTGCTTATAGACTACAAACCTGTACAGCATGTTACTGTACTGAATACCAT1630.24848318546297146No Hit
CAGTGTAAATATGAGCCATTTTAATGGTGATATGCAATTAACTTTTATCGG1620.2469587487423397No Hit
CTTTAGTACATATGAGACGAAATTTGCAAATAAGTTCTTTTATCCGTACAT1610.24543431202170796No Hit
ACATAGATTGGAATTAATGGTCACTTGAAACCGTGATGGTATGTCTAAAAA1560.23781212841854932No Hit
TTACTACCGGAATTGTACCATAATTCGGAATAATCTCCCGACTGTTTGAAA1520.23171438153602245No Hit
ACTGTAGGGTATTATGCTGAGTGAAATAAGTCAATCGGAGAAGGACAAACA1510.23018994481539073No Hit
ACTTGAGTGTGTTAGAAATATAACCTTTGTTGGTGAATTCCTGAAGGAAAG1490.22714107137412726No Hit
GAGTGATGTATGAAATTCTGAGACATTACTGAGAGGACTAGTAGAAATAGT1480.22561663465349552No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCG1440.21951888777096865No Hit
GTTTTGAGAGTTTGATGAGTAAGTAATGACAGATACAACAGCATCTTTAAC1420.21647001432970517No Hit
TCCTAATGGTGTGAAATAGTATTTCACTGTGGTTTTTGTTTTTTAGTATTT1410.21494557760907343No Hit
ATGTAAAACTTCCTCTTGGAAAGCTATTCTGTCTTTGATTTTTCAGAATGT1400.21342114088844172No Hit
ATTGAATGTTACACTTCTCAATTGCTTTATCGCTTCCATTATCAAGAAGAA1390.21189670416781No Hit
ATCTAGACATCATGATTACAAAACAGATTGAGTGTAATAAATTGCTCATTA1380.2103722674471783No Hit
GTACTACTTTAAACATTCTGAATTTTTCTTGCTTTGGGGATTTGTTTTCTT1360.2073233940059148No Hit
GAAATAACTAAAACCACTGGTTCTGATGGAATAATATCCTTTGATGGACTA1320.20122564712338792No Hit
GTAAAAAGAAAACTGTCATTGCTGAAGCAAATAAAAATACACCTTTGGGTG1320.20122564712338792No Hit
CATTCAATGAATCCATTCGATTCCATTCTATGACGATTCCATTCATTTCCA1300.19817677368212444No Hit
AGCTTATATTTTCACAGTGACTATTCACAAAACAAACAAGGGTTTCATATT1270.19360346352022928No Hit
CAATGATATAAGTCAAACTGAAGATAAAACTCATTTCTCACTGAAAATTTA1250.19055459007896583No Hit
GTTAAAGACACTTAAGAAAATGAAAAGATGAACCACAGATTGCAAGAAAAA1250.19055459007896583No Hit
GATCTTCCAGACAGAAAATCAAGACAAAAACATTGGACTTAATTTGCACTC1240.1890301533583341No Hit
GTAGGTAATTGATGTTGAAGAAACATTTTCTTGACCACTGAATAATGTAAG1240.1890301533583341No Hit
GGATAAATAACTACTGCAGTGTTAAAATGTGACTGTTGTAAAGTGAAGTAC1230.18750571663770238No Hit
CAAAAACAGTATGATCATGTCAACTGAAACTGAAAAAGCATTTGATGAAAT1230.18750571663770238No Hit
GTATAAGATGAGTCGTACCATTTTTGTATTAATCCCTTGTCTTTTTCTTTT1230.18750571663770238No Hit
GCTATCTAACAGCTTGATTGCAATATCACATGGTGGATTATTTGGTACTGG1170.178359096313912No Hit
ATACAACAATCTTCCGTTTATTCGTTACATCAATCAATTCTGTTTCAACAA1170.178359096313912No Hit
CAAATCACCTGTAAAAAAGATGATATCTGGTTTCTGTTTCATCACCAATTC1160.17683465959328026No Hit
TTAGTCCACTCATAATCCAAAAATCAGACATGCAAGAGATTTTAGAATACC1160.17683465959328026No Hit
GTTGTATTTATTTGTGTGAGGAATGTCAATGGTATTTTTACAGAAATTGCG1160.17683465959328026No Hit
ATATGAAACTCTAGGCAAGCTCTGTATTTTTGTTTGTTAATGTTTGTTTCG1130.1722613494313851No Hit
GTGGTAAGTACATTACTACTCATATCTATTGTCACATCGGATTGATGCAAA1130.1722613494313851No Hit
GTGTTTTGAAATTCTTGTTGTAAAGATTTTTCACCTCCCTGGTTAGCTGTA1110.16921247599012165No Hit
ACTAATGGTATTCTGGTAAATTTTCAAAACCATAACCCTAAAAACGTGACA1090.1661636025488582No Hit
GTAATGGATGAATAGGCGATTTAATCCGTAATCATCTGATAGTGAACCACC1080.16463916582822646No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1080.16463916582822646No Hit
ATTTCATCTATGAATGTTCCGGTAATCAACATCTTATTCCATTTTTTTTAG1070.16311472910759475No Hit
ATGTTAAATGAAATAAGTCAGAAAGACAAACACCATATGATTTCACTCATA1070.16311472910759475No Hit
ATTTAGTTGTTATCATTTAAAATCCAACTTGAACACCGAACGTGTAATTAG1050.1600658556663313No Hit
AACAAACAAAAAACACTCATGAATACTCACAAATAGTTTCCAGATTCTGGA1050.1600658556663313No Hit
ACTGCATGTTTATTGCAAAATGGTTGAACTTCTGGAAGTAAACATTTGCAG1050.1600658556663313No Hit
GATACTTTCAAAGGAACCAATGTTATGAGTATTGTACTTGAATTCCCTAAA1040.15854141894569956No Hit
GAATGGAATCATCGTAAATGCAATCAAGTGGAATCATCGAATGGAATCTAA1020.1554925455044361No Hit
GTTGTACAGTTGGTTGTACAGTTTCTGTAGGGGTAGTTGTACACTTACTCT1020.1554925455044361No Hit
TATTTATGGAGTACAGGAAATGTTTTGATAAAGGCATGCAATGAATAACAA1000.15244367206317264No Hit
TCTTTATTTTCCGCTATAAGTAGCAATATTTTTTCGGCTACAACTCTTTGG980.14939479862190921No Hit
ATAATAATGTGGACTTTGACAAATCATTTAATCTCTCTGAGTCTGTTTCAT960.14634592518064574No Hit
AATCAATACAGAAACAACAAAACAGTAGAATGGAATAATGAAACTAAGAAC960.14634592518064574No Hit
ATCTATTACATCACAAGACGAGATTTTTAAACCTTAATACCAGATTGCTAA940.14329705173938231No Hit
CCTATAGTCTTCATATGATGTTTTCGTTCTTGAGAACAATTCCTGTGTGAG930.14177261501875058No Hit
ATCTAAGGAAAGACATCGCTAATCAGAACAAAAAAAGGAAGAAGTAATAGA920.14024817829811884No Hit
GACTCAACTAGATTATAACTTCCTAAAGAGCTATCTTCTTCTCTTTAATGT910.13872374157748713No Hit
GCATATAGCAATGTTTGTATTTCTTCAATTGCTTTTTTGAATTGGTCAAAT910.13872374157748713No Hit
ACTACGTACTGATACTAATAACAAGTTATCATCAGAGGAAAAGGCTTTTCC900.1371993048568554No Hit
AAACTTATCTACTTTCAATTGTTCGAAAGCAAAATCGTAAATTTTCTTCTT870.1326259946949602No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA860.1311015579743285No Hit
TACTAGCCCTTTATCTGATAGGTCATTTGCAAATATCTTCTCCCATTCTAT860.1311015579743285No Hit
GTTTGTAGACTTTGTTGATCTTTTCAAAGAGAAAACTTATTGATCTTTTTA850.12957712125369678No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA850.12957712125369678No Hit
AATTAGAGAAGATGAGTCTGGGAGAAAAAATTATTTCAGATTTCCCCAGAA850.12957712125369678No Hit
GATCAGTATGTAGAAATTGAATTGTTGTCACTGAAACAAGTTTTTAAAGAG850.12957712125369678No Hit
TCAATAAAGTGCTTAAAAAAAGACAGACATACAAACCAATGGAACTAAATA850.12957712125369678No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA840.12805268453306504No Hit
TAAAGAAAGTGCTTCTCTTTGTTAAAGTATGTTTCATCAACTAGTCCCATT830.1265282478124333No Hit
ATCATTTACTTGCTCAATAAGTCTATTGAGAAATTTCAGCAAGAAACTGGC830.1265282478124333No Hit
CTTCATAGAAATCCATGAGATAGGGGTTGGTATCTACATTTTCAAATTGTG830.1265282478124333No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.1250038110918016No Hit
AGATAAACGTATTCACGATCTTAAATTATCTCGACAAATTACGTTACAATC810.12347937437116985No Hit
GTATAAGCATGGCAGAGGAAGAAGAAAAAAATAAGGAAGGTTCTTGAGTCC800.12195493765053812No Hit
AACATGATGAAAGAAATTCAAAAAGAACGCGGACTTGCGTATCTATTTATC780.11890606420927466No Hit
AGTATGAGTATCAATAATGAGATGATGATTGACAAGGAAGATGAAGAAGTA780.11890606420927466No Hit
GATTGTTTGTTTTAATCTCTCCTAGATTTGAACATTTTCAGTTTACTCTTG780.11890606420927466No Hit
GTAAAAGTATCAGAGGGCTTATCTGTAAATGCACCTTTATATTTCCCTATT780.11890606420927466No Hit
CACCTACCCTAATAGAGTATGTTTCACTGGTTTTAGCTACTTTATCTTCTT770.11738162748864295No Hit
CATGTGTAACTTTGACAAACATAAAAGCTAAATTACACAAGTGACCAAATG770.11738162748864295No Hit
ATACTACTTGAGAATACACACTTAGCTGTGCATCTGTAGGTATATCAATAA770.11738162748864295No Hit
GATTAAGTGTGTTGGACTTTATTAAGAGAACAGATAAAGTTCGTGATTTTT770.11738162748864295No Hit
GTTAGTAAGTATTGGTCGGTAATTAAGTGTCACGTGATTAGAAAATATGGG760.11585719076801122No Hit
AATAGTTGAATGAATTGAAAGTGTTTAGACTAGAAAAGGAGATGATCATCA760.11585719076801122No Hit
ATATAAAAGAGCTAGGTAAAGGAACACAACAAATATTCCTCCTATAAATCC760.11585719076801122No Hit
GATTTAATGTAGACATATTGGCATTTGAAAGTGCAAATACTGTCTTTATAA760.11585719076801122No Hit
ATAGTAAGTTGTATCAATTAAGTTGCTTTTGGTTACAGGTAGATCAATTCA760.11585719076801122No Hit
TGTCAAGAAAGATTGCATCTATTGCTTGCCCTTGATTGATCAAGTTTAGAT760.11585719076801122No Hit
AAATTGGGAAAGGCTTGCATTAAATCTAATCTGGACAAGGGAAAACCAATG750.1143327540473795No Hit
ATTATGCTACTACTGAAAAAGAATTTTTAGCAGTTGTATTTGCTTGTGATA740.11280831732674776No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA740.11280831732674776No Hit
GTTAACATACAGTGTGATGTTAGTTTCAGAAGCAGAATTTAGTGATCATCA730.11128388060611605No Hit
TGTTTGTACTGAAGCCAAGATTAATCGTAAAGTTTTTTACCAGTGTTGTAC720.10975944388548432No Hit
GTATTTACTAGATGGATTGCCAAATTACTTAACCAAGCTACTTCTCTAAAT720.10975944388548432No Hit
GGTAAGAACACTTAAAATCTATGAAATAGCCTCATTTTCTTTTGATTTTTG720.10975944388548432No Hit
CATCTTAACTTATAAGAACCAGCTTCAGATTTTTCCTAGCTTAATTTCAGT710.10823500716485258No Hit
AATCTATGCACCTCAATAAACATCGGGTTTAGTGGTACATCTTTTATGGCT700.10671057044422086No Hit
GTAAAAACGATCAAAACGTTCACTATTCGCAAATGATCGTACAAAATTAAG700.10671057044422086No Hit
ATATACATCAAAACAAATGCCATTTTTATGCATTTTTGTCCTTACATTGTT700.10671057044422086No Hit
GATTATAGTCACCCGTTAAAGAAAGGTGCTAAATTGGAACTGGGTGTAAAA700.10671057044422086No Hit
CAACTATATTCATCTGTTTGTTTGGTTTTTAGCAGTGGCATTGCTGTTGTT700.10671057044422086No Hit
TATATAATCCAGCTAATTGGTAGTTGTCTTTATAGCTTGTAAATAAACCTG690.10518613372358915No Hit
CAGTAAAATAGCGTAGATAACAGCAGAAGGTGTAACTGTGATTATGAAGCT690.10518613372358915No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA690.10518613372358915No Hit
AATCTAAGCTCTCTAATCAACTTGACTTCTTTGGACCTCAGTAGCTTCACT680.1036616970029574No Hit
GATGACTTTTAAGATGTCGATGAATGTTCCCTCTATCCCTACACTCTGAAG680.1036616970029574No Hit
GATTTAATCTAAGAGGGTTGTATGTTTCCAGGAATTTATCCATTTTCTCTA680.1036616970029574No Hit
TGTTAATGCATTGTATTCCTTAATCGACATAATAACAACTGCATCATCCTC670.10213726028232568No Hit
CAAGTAACACTGGAATTCTTTGACTACCTTAGTTTTTCTAGAGTTTCACTT670.10213726028232568No Hit
TTGCTAAGAGAACTCTTAAAAGATACACACACACATACATCCCATTGTCAT670.10213726028232568No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT660.10061282356169396No Hit
AGTTTATACATTGTGATGGAGGAATTATGAAAGTCTCTTTAGGATGAGGGT660.10061282356169396No Hit
CATTACTTGTAATCGTAATTGTCTTGCCACTTGTAATGATAAAATTTTCTA660.10061282356169396No Hit
AATTTGGACACATCATTGTATTTCTCGTTCTAAGTCCAAGAAGAAAAAGGC660.10061282356169396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAGG551.8189894E-1245.033591
TTCCCAA453.7107384E-1045.033591
GTCATTA206.9589965E-445.0335851
ACTATGT206.9589965E-445.0335851
GGTAACT206.9589965E-445.0335851
ATGTAGC351.1816883E-745.0335851
ATGTAAA253.833598E-545.0335851
ACTACGT253.833598E-545.0335851
GTGGTAA206.9589965E-445.0335851
CCACAAT206.985245E-444.99923728
CTTAAGG351.1888187E-744.99923723
TTGATGC206.985245E-444.99923742
GTGTTAG206.985245E-444.9992379
TGTTTCT351.1888187E-744.99923740
AGCGTTT800.044.99923724
CTATGTA206.985245E-444.9992372
CAGAATT253.8509446E-544.99923733
GGGGATT302.1344713E-644.99923736
GAGTGTG206.985245E-444.9992375
GAGTGTA206.985245E-444.99923730
Produced by FastQC (version 0.11.2)