H23LNBCXX_l01n01_bir_1228_pcontrolb_78.351000000434ff.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_1228_pcontrolb_78.351000000434ff.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	322738
Sequences flagged as poor quality	0
Sequence length	51
%GC	37

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC	11267	3.491067057489357	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT	1184	0.3668610451821602	No Hit
ATATTGGATGATTCCATTCGATTCCATTTGATGTTGATTCAATTCGATTCT	893	0.276695028165261	No Hit
GTGTTAAGATTGTTCGATCTGAGAAGGTTAAGGATGAGATTATTCTTGAGG	856	0.26523062050331847	No Hit
ATCATATCAAGTATCTTTTCTGACCACAATGGTATAAAACTGGAAATCAAT	531	0.16452974239166135	No Hit
GACAAAAACATTGTACCATAATGGGAGTTTCAAAGAAATACCACAAGCTAA	519	0.16081155612292325	No Hit
GTTTAGTGATTGTTGATTGTCACATCAAACAACTTACAAAAGGATCACTTT	502	0.15554412557554426	No Hit
TTCTTGTAATTTTTCACGATCGTAATCTGAAGTAGTATTTTCAACTTGTGC	482	0.14934714846098074	No Hit
ACAAATACATTTGGTGACCACTCTGTGTTACTCCTTTGTCTTTCTTTGGTT	455	0.14098122935632	No Hit
GAACTGAATCAAGAGAGTTTCCTTCATCCTAAATACCACAAAACATAAACA	447	0.1385024385104946	No Hit
GATTACAGATTTGGTAGAAACTAGTTTAACTCAATGAGCAATAACATCACT	447	0.1385024385104946	No Hit
TTATCATCATGTCTCATATGAGAAGTTTCATGTGATGTGTAGCTTCTCCTT	443	0.1372630430875819	No Hit
ATGTTGTTGGTACTCTGTTTTCTTTCTATGGATTTGATCTTGGTTTAAGTT	439	0.13602364766466918	No Hit
GTGTATCAACTATATCTGTTGTTTTATGTCAACTTTTAGCTAGCTTTTAGG	431	0.13354485681884376	No Hit
ACTTGTTCGAAAAGAAACAAAAGAGAATGCTTGGGGAGATAGAAGTGTGAG	421	0.130446368261562	No Hit
CTTTAAAGGCTTTTTTTCATTGTTCGCATACAAAAAGTGAAACAGAATAGC	417	0.12920697283864932	No Hit
ACTTGTTCAAAAAGAAACAAAAGAGAATGCTTGGGGAGATAGAAGTGTGAG	413	0.1279675774157366	No Hit
TATGATTTCAAAGTGGATTATAGGAAGAACAAAGATGGAGAAGACGAAGAA	407	0.12610848428136753	No Hit
GTTATGTTCTTTGCGAAAATAAAAGTTATTCCACAAACGCATTCGATAAAA	405	0.1254887865699112	No Hit
ATTCCATTCCATTCCATTCCATTCCATACCATTCCATTCCATTCCATTCTA	404	0.12517893771418304	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCG	398	0.12331984457981397	No Hit
TACTGATTGTGTCCAACTAAAAACTTAAAACAACACCAGAGATAACGATAA	397	0.12300999572408579	No Hit
AAGTTTAACATAGTAAAGCTGCAAGTGTAATAACCACACGATGAATCCAGT	397	0.12300999572408579	No Hit
ATGAAATAGAATGCAATCATCAAATGGAATCGAATGGAATCATCATCGAAT	394	0.12208044915690128	No Hit
AAACCATACAGTTTTTGGACCTATGTTTGGCATTTTAACCAAGCTATTTGT	394	0.12208044915690128	No Hit
ACTAACAGCCATTAACTAAATGAGCAGAATAAACACCTGAATTAAGATCTG	392	0.12146075144544491	No Hit
ACAGACATATAATCAGTTTTGTTACAGGACAAAAAGATTCATCTAGTACCA	383	0.11867211174389133	No Hit
AGTCTAATCTGAGGAGAAACAAAAATCAAATTGACAAGAAAATTGACACAA	378	0.11712286746525045	No Hit
AAGAGAAACTAACATTTTCAATTCAATGACAAAGCCACATAGCTAATAAAT	375	0.11619332089806592	No Hit
TTTTCTACCTTCTAGAAAGAGGTCAAAAAGTTAATAGCTTCACCGTGAGAA	372	0.1152637743308814	No Hit
GCTTAAATGAGTGCATATACTCATTGGAAAAGAAACAAAGCTTTCCTCTTT	371	0.1149539254751532	No Hit
AACATTGCTTTATGGTCAGAATCAAAGAACTCAAAACTTGAAACAAACTCT	371	0.1149539254751532	No Hit
TAGTTATACCAATCCTCAAGATGTGATGAACGTTGTAATCGATCAGATTCC	369	0.11433422776369687	No Hit
ACTGAAGAGTATCAGAACTGAAGTATCCTTCTCATTCCGGTGAAGAAGAAT	366	0.11340468119651236	No Hit
ACACAATGGTATAGTGAAAGTTGTGACTTGGAACAATCATTATCAGAAGCT	364	0.112784983485056	No Hit
GTTTGAAGTAAAGTCAACAAATGGAGATACTCTTCTTGGAGGTGAAGATTT	360	0.11154558806214328	No Hit
TGATTATGACCGTGATAAGAAGTTTTCAAAGTCCTTTAAAGAATTGGTAGC	355	0.10999634378350241	No Hit
GTTTCCATCATTGTGATTCAGATATTCACAGAGCATACCAGTTTTGTTTCT	352	0.10906679721631787	No Hit
TGAACCTGATAATGGAATTGATACGGCTTATTATTCTGGACTTAGATTTGT	350	0.10844709950486153	No Hit
AAGAAGAAGTCTAAGAAGTGAAGAGGATATATAGTGTGAAGCTATATCGAT	346	0.10720770408194882	No Hit
TAAGATACCTAGGAATAAACCTAACCAAAGATGTAAAGGATCTATACCCTC	343	0.10627815751476428	No Hit
GATACAAGCACATTCATTATGTTTGGTATGACATGATCAGAGTGAATAATA	340	0.10534861094757977	No Hit
GTGGAAGAACAGATAATGACCATTTATACCGGAACAAATGGTTATCTGGAT	337	0.10441906438039524	No Hit
ACCATAAGGATTTTCCAAAAGAGAGAGTACTTACTCTAACACAGTGGAAAA	335	0.1037993666689389	No Hit
GAATAATGTAACTCCAAGCATATTTGCAAACACAGCTAATTGAACGTCAGT	333	0.10317966895748254	No Hit
ATTCAAATGCTTTTAAAACCAACCATGCCTATCAATCAAGTGTGTATAAAC	331	0.10255997124602619	No Hit
GAAGAGAATCCAGATGATGAAGTACCAGAGGTCTATTTAGATAGTGACATC	330	0.102250122390298	No Hit
AAATACAGCTCAACCAGAAACACCAGTTGAAAACAACATTACTGATAAAGA	329	0.10194027353456983	No Hit
CATTTATGCATGGTAACTTCTCTCCTAATGAAGTTTAGCAACTAAGATTGT	328	0.10163042467884165	No Hit
ATCTAAGCATTACTTCATAGCATCCTGAAATGGTGTTGAAACATAGAGTTA	325	0.10070087811165714	No Hit
TGAAAACGGTTTATAAAAAAGAACCTGTGTTTTCGCAAAGCAAAGTTGTTT	324	0.10039102925592895	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACCG	40	6.7848305E-9	45.0031	33
CCCCATG	20	7.0233544E-4	45.0031	31
ATGGTCT	20	7.0233544E-4	45.0031	33
CGACTTG	20	7.0233544E-4	45.0031	24
GCACTTA	20	7.0233544E-4	45.0031	17
CAAAGCG	20	7.0233544E-4	45.0031	17
TCACGTC	20	7.0233544E-4	45.0031	26
GATACTC	45	3.8198777E-10	45.003098	26
AGGGTCA	25	3.8829698E-5	45.003098	19
GCTAGTA	30	2.159608E-6	45.003098	23
TAGTCGG	25	3.8829698E-5	45.003098	28
TACACGT	30	2.159608E-6	45.003098	24
CGCTTGC	35	1.2076453E-7	45.003098	33
CTTGTGC	40	6.7939254E-9	44.996124	45
ACCGGCC	20	7.028719E-4	44.996124	43
GCAAGCT	25	3.8865255E-5	44.996124	43
CGAGTTG	20	7.028719E-4	44.996124	40
CGTGCTA	20	7.028719E-4	44.996124	41
CGTTGTG	35	1.2091186E-7	44.996124	39
GCGTTGT	35	1.2091186E-7	44.996124	38

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content