FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_77.351000000434e2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_77.351000000434e2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209338
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC138576.6194384201626075No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT78293.7398847796386705No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCG16200.773868098481881No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC3630.17340377762279185No Hit
GGTATAACACAGGAACGGAAACATAGTAGAACACTTATTCATCAGGGATTA3490.16671602862356572No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC3420.16337215412395267No Hit
GTATAATCCCTGATGAATAAGTGTTCTACTATGTTTCCGTTCCTGTGTTAT2570.12276796377150828No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTTC253.876677E-545.00621426
TCGGGAG302.1549185E-645.006214
TTCGGGA302.1549185E-645.006213
TGCCGTC14450.044.061345
TCCGTTC750.038.99606736
CGTTCCT700.038.56753538
GTGGGGT301.13581045E-437.50517332
CATCCCC301.13581045E-437.5051735
AGTACGT301.13581045E-437.5051732
GCAGAGC301.13740985E-437.4962237
TCGGTTG301.13740985E-437.4962245
GCCGTCT301.13740985E-437.4962245
CCGTTCC800.036.5588137
CCATCCC250.00210175636.004974
TATAATT250.00210175636.004972
CGGGTTA250.00210422335.9963715
TTTCGGG250.00210422335.9963712
CAGCTTA250.00210422335.996379
GATCGAG458.6616274E-734.9964737
CTTCGGG401.5530288E-533.754662