FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_76.351000000434c8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_76.351000000434c8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306983
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC80412.619363287217859No Hit
ACAAACATAAGTCTCAGCCCTCAAAAATTTCCTGTTATGTCTTTGATTCTT21840.7114400471687358No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT11830.38536335888306517No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCG3850.12541411087910403No Hit
GGAAGAAGGATTTCATTGAGATACTCAAGACTCTTGAATCCTGTCTCTTAT3850.12541411087910403No Hit
GCAAAAGTTTTGAATCAAGCTAGAAGTAGAATGATGAAGAGGAAAAACACA3210.10456605088881143No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGG207.0203136E-445.0063553
GTGTCCT207.0203136E-445.0063551
GGTGACT207.0203136E-445.0063556
CGCGGTT302.1606083E-644.99902329
GCGGTTA302.1606083E-644.99902330
AGCTTCG207.025951E-444.99902313
GCACGCA253.884601E-544.99902336
ATGTCCG302.1606083E-644.99902345
TACGCGG302.1606083E-644.99902327
TACGCAG207.025951E-444.99902324
GTTCGCA207.025951E-444.99902342
TGTTCGC207.025951E-444.99902341
GCCCTCA2500.044.99902317
AACGACG351.2082455E-744.99902343
AATTCGC253.884601E-544.99902329
TAAGGCC207.025951E-444.99902326
CGATGCA253.884601E-544.9990239
GACGCAT253.884601E-544.99902316
CTAGGGC253.884601E-544.99902310
GGGGGGT207.025951E-444.99902327
Produced by FastQC (version 0.11.2)