FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_72.35100000043442.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_72.35100000043442.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7883
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC5346.774070785234048No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT750.9514144361283776No Hit
ATATTGCAACTGATTATTACCACCATAGCATTAGCTAATACCTCCATCACA300.380565774451351No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC230.29176709374603577No Hit
ATTGGTAATGTAGTCAATACTGATGTTGTTCATAGGAAAAGAAGTAACTTC200.2537105163009007No Hit
GAAAGAGAAATTAAGGAGTCAATCCCATTTACAATTGCACCCAAAAGCATA200.2537105163009007No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.22833946467081062No Hit
CCATAGATCATCATAGGGCAAATAAGAAATATGATTCTCATGTTGAGCTTA170.21565393885576556No Hit
AAGATAGATTATGAGTGGTAACTGACTTCCAATATCTGAGAAAAGTACAAG160.20296841304072052No Hit
ATCTTACACGTATGGTTACTATGCAAAATTTATTTCATGATGGAGGTTTTT150.1902828872256755No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.17759736141063048No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA140.17759736141063048No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG140.17759736141063048No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.16491183559558545No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA130.16491183559558545No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.16491183559558545No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.15222630978054041No Hit
CTCTTAGATTGGTTAAGTTTAAAATGTTCACCCTTAATCTGATTTGGCAGT110.13954078396549535No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA100.12685525815045035No Hit
TTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT100.12685525815045035Illumina PCR Primer Index 11 (95% over 21bp)
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.12685525815045035No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA100.12685525815045035No Hit
GTTATAGTATGCATACATTTTGAGCATGCCATTCTTTTTTGTTTTAAAACG100.12685525815045035No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC90.11416973233540531No Hit
AGAATATGTAGCTGGTAAAAATGAGTTATGTGCTCTATCTTGGAAAGGTGT90.11416973233540531No Hit
ATGATTGTTAGCACCTTCATTACACTATTCATACGATTACTTACAGACGAC90.11416973233540531No Hit
CTATACCAATGAGCAATAAGCAAAAAAATCTTATCCGTAACATATTCACTT90.11416973233540531No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG90.11416973233540531No Hit
AACGTAAGTTTTAGATAAACCACTTTGGGAATTTTAATGATTTCTTCAGTT90.11416973233540531No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGC90.11416973233540531No Hit
TAATTTTATTTTGCGTTTTAACGTATTTCTCAAGCCATTGATATTGCTCCC90.11416973233540531No Hit
CATTTTTAGGTTGAATTTCACATGAGAGAAGTCATTCTTTGTGTTGTGTTC90.11416973233540531No Hit
GTGTTAAAGAGTCTTTTTTATCTGAGTCGAAGAATTGTTCTTGTCCAAATG80.10148420652036026No Hit
GTTGATTGTCTTTTTTTCATTCAAGTTGAAATTATCCTGGTTTTTGGTGTG80.10148420652036026No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA80.10148420652036026No Hit
GTAATAGGTGTTTCACTCAATTCTCGAATAGTATGAATACCCATCGTTTTC80.10148420652036026No Hit
AAGTTGACAAAATTTGGAATAGACTTGATTAAGAAAGTAAGAGAGAAGGCT80.10148420652036026No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACC80.10148420652036026No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC700.041.78571345
CTATATC750.039.033
ACGCTAT750.039.030
TATATCT750.039.034
CGCTATA750.039.031
GACGCTA750.039.029
AGACGAC750.039.025
GACGACG750.039.026
CGACGCT750.039.028
ACGACGC750.039.027
CGAGACG750.039.023
GAGACGA750.039.024
GTATGCC800.036.56250442
ATCTCGT800.036.56250437
GCCCACG800.036.56250418
CCACGAG800.036.56250420
TATGCCG800.036.56250443
GAGCCCA800.036.56250416
CACGAGA800.036.56250421
CCCACGA800.036.56250419
Produced by FastQC (version 0.11.2)