FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_71.35100000043428.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_71.35100000043428.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110674
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC91978.309991506586913No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT38783.5039846757142596No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG9300.840305762871135No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC2140.19336068091873432No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC1710.15450783381824096No Hit
GTACCAACATTACGAATTGATGTTATACTAACGCTGTCTCTTATACACATC1230.11113721379908559No Hit
ATTACACAGTCTCAGGTAGTATCTTTATAGCAGCATGAGAATGGACTAATA1180.10661944088042358No Hit
ATACTTCAGAGTATGTACACTGTCTATAACCTGTCTCTTATACACATCTCC1150.10390877712922637No Hit
ACATATACTTCTTCCTCAATCTTACCATTGAGGAATGCACTCTGTCTCTTA1150.10390877712922637No Hit
GTTATGGTTCATTTGATCGTATGGTTTACTTGGATAACTGTGGTAATTCTG1130.10210166796176157No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGTTC206.992823E-445.0167330
CGGAATT207.0084236E-444.99638745
AACCGTA207.0084236E-444.99638739
CCAGCTG207.0084236E-444.99638740
CGATTGT207.0084236E-444.99638727
TCCTACC207.0084236E-444.99638724
AGTATCG207.0084236E-444.99638710
TCGGAAA207.0084236E-444.99638727
AACTGGG207.0084236E-444.99638725
GGACGAC207.0084236E-444.99638724
TGTAGAG207.0084236E-444.9963875
ACCGTAT207.0084236E-444.99638740
ACATAGC207.0084236E-444.9963878
CTTAGCC207.0084236E-444.99638717
AAGTCGG207.0084236E-444.99638724
ATGAGAC302.1488868E-644.9963845
ACTAACG302.1488868E-644.99638427
GCACTCT253.8697523E-544.99638437
TGCCGTC9500.044.5227445
CTAACGC356.1814426E-638.5857728
Produced by FastQC (version 0.11.2)