FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_70.3510000004340e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_70.3510000004340e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96868
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC50865.250443903043317No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT18551.914977082214973No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG4190.4325473840690424No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC5500.044.5878445
AATAGGA301.1292378E-437.5167852
ATGCCGT6800.036.0636944
ACTATTG250.002093007836.0161173
GGATCGA250.002093007836.0161176
TATGCCG7300.033.5935843
CGTATGC7350.033.3650541
CTCGTAT7350.033.3650539
CGCTATA7350.033.3650531
TCGTATG7350.033.3650540
CGAGACG7350.033.3650523
GACGACG7350.033.3650526
ACGAGAC7350.033.3650522
CGACGCT7400.033.1396128
GCTATAT7400.033.1396132
CACGAGA7400.033.1396121
GTATGCC7400.033.1396142
GACGCTA7400.033.1396129
ACGACGC7400.033.1396127
AGACGAC7450.032.91719425
Produced by FastQC (version 0.11.2)