FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_68.351000000433c9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_68.351000000433c9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66297
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC34165.152571006229543No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT13382.0181908683650844No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG2570.3876495165693772No Hit
ACAAACATAAGTCTCAGCCCTCAAAAATTTCCTGTTATGTCTTTGATTCTT2540.38312442493627163No Hit
TTTGTGTATACTGTTGGACTGTTGTAAGTAGCTGTTGTATGTCTGTCTCTT730.11011056307223555No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTACT206.988668E-444.99547219
TCATTAC253.8532966E-544.99547221
TCAGCCC406.6556822E-944.99547214
CACGTAC206.988668E-444.99547223
TGCCGTC3600.044.99547245
AAGTCTC406.6556822E-944.9954729
CAGCCCT451.8831997E-839.99597515
CTCAGCC451.8831997E-839.99597513
AAGGTGT356.131948E-638.5966766
AATGAGT301.12400434E-437.5245481
GTACTAA250.002086116536.0235671
AAAGGTG504.73392E-836.0235675
GTTGCAT250.002086116536.0235673
AGCCCTC504.7655703E-835.99637616
GTCTCAG504.7655703E-835.99637611
GCCCTCA504.7655703E-835.99637617
TCTCAGC504.7655703E-835.99637612
GACTGTG250.002093872335.9963767
CTGTTAT458.543284E-734.9964832
ATAACAC352.7812493E-432.16393
Produced by FastQC (version 0.11.2)