FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_67.351000000433a0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_67.351000000433a0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132043
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC60884.610619267965738No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT10580.8012541369099461No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG3550.2688518134244148No Hit
TAAGAGAATCATTCATTACGTGTACTTACAACAATCTGTCTCTTATACACA2470.1870602758192407No Hit
TAACAGTTTCATCAATAGAAACGTCTCTTGAGTTATCGTATGAATTGTACA1460.11057004157736496No Hit
GTGATAAGCATTGGGTGTTATATGTAAGTGATGAATCACTAAACTCTACTC1430.10829805442166567No Hit
CATATGAATCACACAATAGTTACAAGACTGTCATATATGATGACATACACC1410.10678339631786614No Hit
CTTTAATTCGCTAGTGATTACACTAGATGTACCATGGATATGAACCTTATC1410.10678339631786614No Hit
ATACTTAACTCCCATTTCTATATTCCCTGAACTTCACATTTGCTCTAACTT1380.10451140916216688No Hit
GTATAAACAACACACAATACAGCAAGTGCAACAAAATAGATTTGTCTAAAG1370.10375408011026711No Hit
AACAATCTCTCTTCTAACAATCTGCCTTGAAGATACACTTCCAAAAACTCA1340.10148209295456784No Hit
CATCAAAGTTATCCAAATATCGATGATTTCCATACCTTTTTGCTAAGAAAT1340.10148209295456784No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAGC302.1453925E-645.0166741
TCGTTGA207.010881E-444.99962210
TCGCAGG207.010881E-444.99962234
GGGCTTT207.010881E-444.99962211
CGTATGA207.010881E-444.99962237
GGTGTAG207.010881E-444.99962227
CAACGTA207.010881E-444.99962234
GTGGGCT207.010881E-444.9996229
CGTTGGA207.010881E-444.99962242
TGTGGGC207.010881E-444.9996228
TTGTCGT207.010881E-444.9996227
ACCGAAA207.010881E-444.99962242
AGGGTGG207.010881E-444.9996226
TAGCCAC207.010881E-444.99962214
AAAACGG207.010881E-444.99962232
AAAGTCG207.010881E-444.99962211
GTTGTCG207.010881E-444.9996226
ATTCACG207.010881E-444.99962211
TACGAAT207.010881E-444.99962217
GTCGTTG207.010881E-444.9996229
Produced by FastQC (version 0.11.2)