FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_66.35100000043386.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_66.35100000043386.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76637
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC3062639.962420240875815No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT1684821.984159087646958No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG32954.2994898025757795No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC1330.17354541539987214No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA950.12396101099990867No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC840.1096076307788666No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA830.10830277803149915No Hit
TTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT790.10308336704202932Illumina PCR Primer Index 11 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC33100.044.85949345
ATGCCGT50650.029.36029644
TCTTATA28550.027.9743941
TATGCCG53900.027.58996243
ATCTCGT53450.027.56969837
TCTCGTA53450.027.56969838
TATCTCG53450.027.56969836
GTATGCC54000.027.53887242
CTCGTAT53600.027.5345239
CGTATGC53850.027.53202241
TCGTATG53700.027.52513940
GACGACG53950.027.52269226
ACGACGC53950.027.52269227
CTATATC53750.027.51749433
CGAGACG54150.027.50413323
GAGACGA54150.027.50413324
ACGAGAC54250.027.49490422
AGACGAC54100.027.48796725
CGACGCT53900.027.48268128
ATATCTC53550.027.47620235