Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l01n01_bir_1228_pcontrolb_66.35100000043386.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 76637 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 49 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC | 30626 | 39.962420240875815 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT | 16848 | 21.984159087646958 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG | 3295 | 4.2994898025757795 | No Hit |
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC | 133 | 0.17354541539987214 | No Hit |
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA | 95 | 0.12396101099990867 | No Hit |
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC | 84 | 0.1096076307788666 | No Hit |
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA | 83 | 0.10830277803149915 | No Hit |
TTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT | 79 | 0.10308336704202932 | Illumina PCR Primer Index 11 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 3310 | 0.0 | 44.859493 | 45 |
ATGCCGT | 5065 | 0.0 | 29.360296 | 44 |
TCTTATA | 2855 | 0.0 | 27.974394 | 1 |
TATGCCG | 5390 | 0.0 | 27.589962 | 43 |
ATCTCGT | 5345 | 0.0 | 27.569698 | 37 |
TCTCGTA | 5345 | 0.0 | 27.569698 | 38 |
TATCTCG | 5345 | 0.0 | 27.569698 | 36 |
GTATGCC | 5400 | 0.0 | 27.538872 | 42 |
CTCGTAT | 5360 | 0.0 | 27.53452 | 39 |
CGTATGC | 5385 | 0.0 | 27.532022 | 41 |
TCGTATG | 5370 | 0.0 | 27.525139 | 40 |
GACGACG | 5395 | 0.0 | 27.522692 | 26 |
ACGACGC | 5395 | 0.0 | 27.522692 | 27 |
CTATATC | 5375 | 0.0 | 27.517494 | 33 |
CGAGACG | 5415 | 0.0 | 27.504133 | 23 |
GAGACGA | 5415 | 0.0 | 27.504133 | 24 |
ACGAGAC | 5425 | 0.0 | 27.494904 | 22 |
AGACGAC | 5410 | 0.0 | 27.487967 | 25 |
CGACGCT | 5390 | 0.0 | 27.482681 | 28 |
ATATCTC | 5355 | 0.0 | 27.476202 | 35 |