FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_64.35100000043343.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_64.35100000043343.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2262
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC753.3156498673740056No Hit
GGATAGATAAGAATAACGGCCAATTGATGGTAGCTATCAAAATCAATAAAC80.3536693191865605No Hit
AAACAAGTAAACAAAGAGCAGACAGGCTTGCAATGACTAAGGCAAGAGAAA70.3094606542882405No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT60.2652519893899204No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.2652519893899204No Hit
TAACAGAATGGTAGAATTAAGAAGGCTTCGCATAAGTAGTGTAAATAATGG50.22104332449160036No Hit
GTGTTATTTGCTTTAAACTCTGAGCTATGTTTAGAAAGCAACATGTTTTTC50.22104332449160036No Hit
ATCTTATAGCTGGTTTCATATAGTCAGCGAATGATAAGAATGTTGCTGTAT40.17683465959328026No Hit
AAGTTAAGCCTTAAATTTCTTTCATGGAAGCCTTCAAAAATAGTTATGGTC40.17683465959328026No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.17683465959328026No Hit
GTAATAGATCGTTTTCATGTTGTTAGATTAGTGTGTGATACCATGCAACAC40.17683465959328026No Hit
ACTTCAAATTGATTGGGAAAGAATGTACCAAATGAATAGTCATAATCCATT40.17683465959328026No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.17683465959328026No Hit
TAAGTGAACTGATAGAAGTGTTCAGTATTGTTTTTTCTGGTGTTAATATGG40.17683465959328026No Hit
TCATAAAGCTTGAAGTTTTACTTTCTCGTTAGTATGAATTCTGCAATATCC40.17683465959328026No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA40.17683465959328026No Hit
GAGGTAAACAATGCTTGAGATTACTAGTATAAAAGACGGAATCGTAATAGA40.17683465959328026No Hit
GCTTTTAGTAGTTTTGATATCTATGACGTTTTTCTGCTTATGAGAAGATTT40.17683465959328026No Hit
TACCTGCTGTATTTCCTAATTCATGGTTCATCAATACTGTAAATGTACCGT40.17683465959328026No Hit
GCATATTACAAGACAAATCGGCATACATAGGAATTGCAGTATTGTACAGTG40.17683465959328026No Hit
TAATAGGGTAGTGGGTTAAATTGATCAAACCTTTGGAAAGTTCTACAACTT30.1326259946949602No Hit
GTAGATGGTGACTATTACACTAAAAAGCCTGATGTGGATAATTTGGCAAAA30.1326259946949602No Hit
ACAAATAAAGGGGCAGAAGAATTTCATACTGAAATAAGGACTGTGAAGAAA30.1326259946949602No Hit
ATGGAACAGTCAATGAACTCGAATGGAATCATCATTGAATGGAATCGAATG30.1326259946949602No Hit
CAAATGGTTTTAGCACAGCTTATGATAGTACTGGAACTAAAGATTTTGATA30.1326259946949602No Hit
GATATGTACGCCTTAGTGATAGTAAGGTTAAGATCAGTAAGGATTTGTACA30.1326259946949602No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.1326259946949602No Hit
TACAACACAGTGTTAAGCATGGATTCATAGAAGAGACAATATCCCATCCAC30.1326259946949602No Hit
CAATTCAAAGGTTATCATGAAGCTTGCAAGATTTTTGATGAAACTTAGTCA30.1326259946949602No Hit
ATAGCTAGAGGTCTTCCATTTTGGATGTGATATCATAGTACAAAAACCCAT30.1326259946949602No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA30.1326259946949602No Hit
ATACCATTCGATTCCATTCGATGATAATTCCATTCGATTCTATGCGATGAT30.1326259946949602No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA30.1326259946949602No Hit
GTATGTAATGGTACCCCTATACAATTATTCGATAGATCGTCTTTTCCCTCA30.1326259946949602No Hit
CTTACTATCTTTTCAGATATCAGTGATGGTAAATTTGATATTACCCGTGAT30.1326259946949602No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.1326259946949602No Hit
ATTGTAATGTTTAATAGCTAGCTTTGCAATCATTTGTATGCCAACATTGAA30.1326259946949602No Hit
GTTAGATTTTGGCTCATAACGTTCTAGTTTTAGTTTTGTGAGAAAAACCGC30.1326259946949602No Hit
AGTTTATTCTGCTTATTATGTTTCTTCATTGTTGATCTCCTTGGGGAAATG30.1326259946949602No Hit
GGTAAAAATTGCAACGTTGTTATAGAATTTGCTACGTCATAATACCGATTG30.1326259946949602No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC205.6362164E-445.045
AGCCCAC250.00169196235.99999617
GTATGCC250.00169196235.99999642
AATCTCG250.00169196235.99999636
ATCTCGT250.00169196235.99999637
ATCTCCG250.00169196235.99999610
GCCCACG250.00169196235.99999618
CCACGAG250.00169196235.99999620
TATGCCG250.00169196235.99999643
CATCTCC250.00169196235.9999969
GAGCCCA250.00169196235.99999616
CACGAGA250.00169196235.99999621
CCCACGA250.00169196235.99999619
ACATCTC250.00169196235.9999968
TCTCGTA250.00169196235.99999638
ACGAGAC250.00169196235.99999622
ATGCCGT250.00169196235.99999644
CGTATGC250.00169196235.99999641
CGAGCCC250.00169196235.99999615
CCGAGCC250.00169196235.99999614
Produced by FastQC (version 0.11.2)