FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_63.35100000043329.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_63.35100000043329.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93769
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC26192.7930339451204556No Hit
AAAGATTTCTGCAGCTAAAACCAAGTATTACAAAAGCTTAAACATCTTCCT5600.597212298307543No Hit
GTACAAATCCTTACTGATCTTAACCTTACTATCACTAAGGCGTACATATCT4850.5172285083556399No Hit
AAAGAATCCTTTGTACTAGTCGATGTGATTTTGTTTTCAACTGTTCTTGCT4150.44257697106719707No Hit
ACATTATCAATAGCAGCAGTACTTACAAAACGTAGAAATTCCAAACGAAAC4050.43191246574027664No Hit
GACTATAACATGTGAGAAAAAGAATCGTGGTGTGTTCAAAGATAGAACTGA3790.4041847518902836No Hit
GTATTCCCAAGAGAGAATAAAAAACACAACAAGATTGCAAAATCTATTCGA3330.35512802738644966No Hit
ATAGTTTACTGAGAATGTTGATTTCCAATTTCATCCATGTCCCTACAAAGG3170.338064818863377No Hit
GTTGTATCAGTATTGCTATTTGTACTTGCTTCAATAAGGTCTTCGGTAATA3150.3359319177979929No Hit
CATCTTATGCTTTATCTTGTTGAATGTTCCTTATTTGCATCATAGATACTG3030.32313451140568844No Hit
ACAATAGAATGGTGTATGATCCCTTCTTTTTCATAGCTCTGTTTCTAATCT2890.30820420394799986No Hit
GGTTTCAATCATTCATTCTTTCATTCATTCCTTCTTCATTCAAGAAATAGC2820.3007390502191556No Hit
GAAATACTCTTGATGTTGGGATAGAAAGGAAATTCTGAGATACTTGGCAAA2590.27621068796723863No Hit
GTTATTTAGAGAATGACAAGTGACTTAACATTACGTTTCCAGATACCAAAA2500.26661263317301026No Hit
GGTTAACATAAACTTGTAAAGGCTATAGATATCTGGTTGGTGAGTCTGAGT2490.26554618264031826No Hit
GTTCTATTCTATTCAATGGTGATTCCATTCGAGTACATTAGATGATTCCAT2350.2506158751826297No Hit
AAACATTGAAGTAGAAAATGAAGCTTTTGATCGGTACATGAGTAATGTGAA2350.2506158751826297No Hit
ATACTAAAGCACAAGAACCTGTTAAAGATAGTGAGGCTGTTTTGTAAGCCC2350.2506158751826297No Hit
CTATTAGTGTTAATTCCATTCCATTCCATTCCATTCCTTTCCATTCCCTTC2230.23781846879032514No Hit
TCAAAACACTAATTCAGAAGAGAAGACTTTAGAGTCGATGGAAAAATTTGG2120.2260875129307127No Hit
ATCCATAACAATCCAACAACAAGGAATAACTTAACTGAAACAAGTTCAGAC2050.21862235920186843No Hit
GTCTTAAAGCAACAACAAAATTAAGACTCCAAATGCATAATCCACTAATCC2020.2154230076037923No Hit
CGATTCATATTTGTGATAAACGCAAAATATAGATTCGATTCAAACAAAAAG2000.21329010653840821No Hit
TCGTTATCTCTGGTGTTGTTTTAAGTTTTTAGTTGGACACAATCAGTATTC1970.2100907549403321No Hit
ACAAAATACAGTAAACACCTTACATCATCTAAGGAGTTCATGCCACTCATT1920.2047585022768719No Hit
AGTGATAGATGGTATTGAATCAGATTATTTCATGTTCTTAGATGCTGGAGA1890.20155915067879576No Hit
GTATGTGTATTATCATGGTAAAATGTCCCATCAAATCTGCAATAATCATGA1820.19409399694995147No Hit
AATGTATCCCTGTCTATTAGTTACCGTAATATGCCAAAAGGTAATGTTCCT1800.1919610958845674No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT1780.1898281948191833No Hit
GATGTTTGGTTTCTTTATCCACCTAAGATGTTTGGCTTCTTTTTCTTATCT1750.18662884322110718No Hit
CTTCAAAATATCTCAAACGTACTAATGTTTGGTACTTTGGAGGCAACATAC1720.18342949162303107No Hit
AGACAGAGAAAGAGAAAGAGAGATCATTCCTATTGAAAACAAGAATAAGTC1720.18342949162303107No Hit
AATAAGAAGTGTGTTTGAAAGATCTCCAAACACTGTTTTATAACCTCTTTG1700.181296590557647No Hit
GATAAATCCAATACAAACGAAGAACATAAATCAGAAAACCAAAATCTTTGC1660.17703078842687883No Hit
AATCTGGAGGTAGGAATCAATTTTAAACCAAAACTTATGCTCAAACGTTTA1630.1738314368288027No Hit
TATCTGAATGATTAGATGAGTATAGTGTGAAGGTTAAACTACTGCTACATA1610.17169853576341862No Hit
ATTCCATTCCATTCCATTCCATTCCATTCCATTCCGTTCCATTCCATTCCA1590.16956563469803454No Hit
TAATCATCCTGCTGTGCAGTTAATAAACTCATCCAGTATGTATGTATGTGT1590.16956563469803454No Hit
TAGTTTAGCATTGCCTTAAGATAGTAAATCCAGAAAACAACTGAATCAAGA1570.16743273363265046No Hit
ATGAAGAGTAGATGGATTTTAACCAAATAACGAGTGATTGATACAATGAAG1510.1610340304364982No Hit
ATTTAGTGTTATTGTAACATGAAAGCGGTCTTGCAACTAGGAAAGAAACAT1460.155701777773038No Hit
GGAGGATGGTAATTCCTTTAATTAGGTTTGGGATTACCAATGAATGTTCTC1440.1535688767076539No Hit
ATCTTGAAAGGATTGATTAGTTTCAAAGGGTATTTGGCAGAGGAAACTGTA1430.15250242617496187No Hit
GAGTTAGGATATGATTTACGACATTGAATCTTGGACTGATTAATTCTTTGC1420.15143597564226985No Hit
GAGTACATTTAATGATTGCTTTGTTTTCAATTCGAAGATGATTACATTGGA1410.1503695251095778No Hit
GGTTTGGGATTACCAATGAATGTTCTCTCTCTCGCTTGTTATGCTTCTACT1400.14930307457688574No Hit
ACCTGATATATCGTTTTCGCTTTTATTTTCAGGTGTTCTGAAACTCATTTG1400.14930307457688574No Hit
GAGCATCATATTTGAAGACAATTCCATCCGAACTTGAAACATATGAAAAAC1400.14930307457688574No Hit
ATGAAGAGTAAATCAGTCAAGCCTTTTTTATCTCATGCCTCTTGTAGCTTT1350.14397082191342556No Hit
ATCCTAGAGGTAAAGTTTTTAGTCTTTAACCACTGAGTATGATGTTCACTG1290.1375721187172733No Hit
TATAAAGCGAGATATGAAACTTTTCTCTGGGAGTCTTCAGAATCATGTTCA1290.1375721187172733No Hit
AGTTACAGGAATCAATTTCAAATCAATGTTTATGCTAACGGTAATCTAACA1240.1322398660538131No Hit
GATCTGTGTGGTTAAGTGAGATTACAGACTTCTTTCAATACCTCAAATCTT1240.1322398660538131No Hit
GTTATATGTAACGTTCAAGTAGTTTTCTGGAATATCTGAATAAGAAGCGAA1240.1322398660538131No Hit
CTATCAATTTTTCTCGTCTTTTTCTTGTTATTCCCAGATGATTTACCTTGG1230.13117341552112105No Hit
GTCATTTACAGTGATTCTGATGCTCTTACAAAACGGGTTTCATCTTCAAGG1230.13117341552112105No Hit
CATTAAAACATTACAACACTAAAAACAACAACAAGTACGATGAGTACGAGA1220.130106964988429No Hit
CATCAAAAAGAGTTGGAGAGGAAACAGAGACATCTATTTTTCTTAACCTAA1210.12904051445573697No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCG1210.12904051445573697No Hit
TAATGATTGTTTGTCTTGGTATGGGTTTCTGTGTTCATTATCTACTGCTAT1200.12797406392304492No Hit
AGATAATACATCTCTTCATGAATCGAGTTAACAGTATCAAGAAGACTCTTT1190.1269076133903529No Hit
GTTTAATTAAGCAGTTTTTGATGGATTATGTAAACGCACTACTTGCATTCT1180.12584116285766084No Hit
GTTAAGATTGTTCGATCTGAGAAGGTTAAGGATGAGATTATTCTTGAGGGA1150.12264181125958473No Hit
GTTTCAAATGGGTTTCTCAATACATCAAGTAAGACAAACACACGCTGTGTT1150.12264181125958473No Hit
GAATTATTACTTCGCTAGTCAATAAAACTTCTGGGAATGTCAAAGTATTTG1150.12264181125958473No Hit
ATACAATACCATGTTGTAAGGATTTATTAGAACCTGTCAATAACAACCCAC1140.12157536072689268No Hit
GACTCCAAACATTATCAATAGCAGCAGTACTTACAAAACGTAGAAATTCCA1110.11837600912881657No Hit
ACATCTAAGGGTCTTAAGAGAAACAAGTGAGAAGAAAAAACAGTTACGGTT1110.11837600912881657No Hit
AGTTTTCTCAAAAGTTTTGAGAAACTTTTTGATGTTGGTGTTTTTAAGTTC1100.1173095585961245No Hit
AATGTATACAGTGTTTCCATAATAGATGCCGATGCTTCTAAATCATCAATA1100.1173095585961245No Hit
GATGTTACCACTTTTTACTAAGTGACCCCAGAATTGTTTTGAAATCTCGAA1080.11517665753074044No Hit
ACAACATACCAAAACTTTTGGGATGCAATAAAAGTTGCACTAAGGGAGAAA1060.11304375646535635No Hit
CGTAAAGAGTTATGGTTTCTTGTTGCAAAATCGTTATTATGATTCCTAAGC1050.1119773059326643No Hit
AGAGATGTATGAAAACTCTTGTGTAAGAAGTCTGTTATAATCGGATATTTT1050.1119773059326643No Hit
GAATCCAACAAAGCAAGCTACTGATTCAAAAGAATCCAGTTCATGATACTT1040.11091085539997228No Hit
GAAAACATCCAGTTTATTTAAATTGCAGTTGTCGTAAAGTATGCATGAAGT1040.11091085539997228No Hit
ACATAGAAGATAGCTAAAATCCACATCGAAAAATCACACAAGACAACAAAT1030.10984440486728024No Hit
GAATGGAATCATCTAATGAACTTGAAAGGAATCATCATCAAATGAAGTCAA1020.10877795433458819No Hit
GTTCAATATGGTCCAACATACTTGAAGGTTTATCGACTTCATCAGTAACAG1020.10877795433458819No Hit
AGACATGAGAACTTATGTTTTGATTTTGAGTATTTGAAGTTTGGTTATGGA1010.10771150380189615No Hit
GTAAAAGAGGAAGAAGATAAAGGGAATCAGAAGAATGGTGGAGATGAACCT1000.10664505326920411No Hit
ACTATGTTCCTTCTCTGAAACTGGATATTGTGGTTCGTTCTCTCGGATGTT1000.10664505326920411No Hit
ATTCAAGATCATACGATTCTCTTAAACAAGGGAGCATAAGATTCCGGAAAA1000.10664505326920411No Hit
CATCTATTTAGGTAGAACCTTATTGGTTACCATTTCAAGGGATGATAGTTT990.10557860273651208No Hit
AACCAAAACTCATGTTTGTTCACTCCAAACACAAACACAGCAGTAATCAAA980.10451215220382001No Hit
AAAGAACAGAGACTAGACTAGATACAAAAGGTTCCTCAGCATTTGATTTAA980.10451215220382001No Hit
ATCAGATACATGATTTGCAAATACTTTGTCCTTGTCTGTTGGTTCTCTTTT980.10451215220382001No Hit
ATCTTGAGGATCCAATGGTAGCTTTGTGTTGTTTCTCTTTGATGTTTTCTG970.10344570167112799No Hit
GTTTATGGCATGATAATCTTTCAATCCTGAATGCTAATAAAAATGCCAAGG960.10237925113843595No Hit
GTTTAAGGTTGAAGGCAAGGAAGAGAAAGAAGAAGGGGAAGATGATGAAGA940.10024635007305185No Hit
AGTGGATTAAGTGTTATGAATGATGTAAACTCAGTTCTACTGGTGTGTAAA940.10024635007305185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGAAT453.7471182E-1045.021884
TGTGTAC302.136987E-645.021883
CTAGAGG206.983387E-445.0218774
ATCTAGG206.983387E-445.0218771
TTTACAC253.8522736E-545.0218773
TGTGTCT206.983387E-445.0218774
ATCTCGA253.8644444E-544.99786844
GGTAATC351.1964767E-744.99786840
TTAATGC253.8644444E-544.99786841
GGCGTAC650.044.99786839
AGGCGTA650.044.99786838
AGTATAG453.765308E-1044.99786819
AACGGTA351.1964767E-744.99786837
CACTCAT351.1964767E-744.99786844
TGACCCC253.8644444E-544.99786823
TCCACTA351.1964767E-744.99786841
ACTAATC351.1964767E-744.99786844
CGTACAT650.044.99786841
TATAGTG351.1964767E-744.99786821
TGCTAAC351.1964767E-744.99786833
Produced by FastQC (version 0.11.2)