FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_62.3510000004330f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_62.3510000004330f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184475
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC75034.0672177801870175No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT16180.8770836156660794No Hit
AGAAGATCCAACATAGCTTGCGTAAATGATCACTGTCTCTTATACACATCT7260.3935492614175362No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCG4580.2482721235939829No Hit
ATATAATTTCTCGCCTAAACATAAACAGATCTTTAGGTACGAACTTGAAAC3880.21032660252066673No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAT207.017877E-445.036
TCGCCTA502.1827873E-1145.011
ACGTTGG207.017877E-445.037
GCGGTCA207.017877E-445.013
GGACTGA253.878038E-545.06
TGGAAGG253.878038E-545.033
AGGACTG253.878038E-545.05
CCTACGG207.017877E-445.015
AAGCGGT207.017877E-445.011
GCGTTAA207.017877E-445.011
GCACCCC207.017877E-445.036
ACCTAGC207.017877E-445.039
TCGGACA207.017877E-445.035
TGACCAC207.017877E-445.012
TTCCAGG253.878038E-545.041
CTACGGA207.017877E-445.016
CTTGCGT1350.045.017
TCTCGCC502.1827873E-1145.09
CTCGCCT502.1827873E-1145.010
AGTTCCG253.878038E-545.010
Produced by FastQC (version 0.11.2)