FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_61.351000000432e4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_61.351000000432e4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167692
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC22701.3536722085728596No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT6860.4090833194189347No Hit
AAACAGCATACCTTAAGTAAGCCTATAAGCATTATTGGAACTGGTTTACAT2620.15623881878682347No Hit
GAAGTAATGAAGTACTGATACATGCTACAAGATCTGTCTCTTATACACATC2520.1502755050926699No Hit
ATGGTAATGAGAAAAGTGTAAGAGTAGACTAGTCATACCTGTCTCTTATAC2420.14431219139851634No Hit
TAAGACTACTGTAGAATAGTGCGTATAGGAATACAACCTGTCTCTTATACA2300.13715621496553204No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCG2300.13715621496553204No Hit
TTGTTACACAAGAACAGACAAATTGGTACTTGATACAACAGAAAGTCTGTC2270.13536722085728597No Hit
GTCTTTTGGAGTAAGTCATGTCTAAGAAGTAAGTGTATGTTTGTCTTTTGC2250.13417455811845527No Hit
ATAATCCCTGTCATTTACTTTACCATTGATGCCAATATTCCTGTCTCTTAT2230.13298189537962454No Hit
CATCAATTCTGTCAATGTGTGAATACGTTAGAATAGTTTGTCGTGTGTTGT2210.13178923264079384No Hit
TCATATGGTTCTTGTTTTTCTCTTGTTGATGTGATCTATCATGTTGATTGC2100.1252295875772249No Hit
CACAACAACAACAAACAAACAAACTCAGTCTAGTGTAAGTGAGAAAAGGAA2040.12165159936073278No Hit
ATTGTACTCTTCTTCTATCTTCTCCTTCTTCGCTATTGCTATTCGTTTCCT1990.118669942513656No Hit
GTAGTGTATGGTCTAAAACTCATACAAATGTCGATAGTGTTACAAAACAAG1980.11807361114424063No Hit
TGTTTTCAAGAAGCTTGTCTTAAGATATCTTCCTGTCTCTTATACACATCT1870.11151396608067171No Hit
GAAATAGACTCAGTGTTTGGTAGAGTTTATGAAGATAGTAAGATGCAAAAG1860.11091763471125635No Hit
ATTCCATTCCATTCAGTTCCATTCCATTCCATTCCATTAAATTTCGTTCCA1840.10972497197242563No Hit
CTATTTGAATAAGGTCTTGTGTGTTTTGTATTACTGTCTCTTATACACATC1770.10555065238651813No Hit
TACCAGTACAATCTTGAGATCATGGATGTTTATCATAGGAAATGGTATCTG1740.10376165827827206No Hit
GAGTGATGTTATGGTAATATGCAAATCATTCCTTTGTATTCGAATAGCTCT1720.10256899553944135No Hit
GTACTTCCTAGTAATGCCAGTAAGTTTGAGGACACATACATTGTATCTGTC1700.10137633280061065No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG207.00596E-445.0131261
AGTCCCG253.876668E-544.99970222
GATCGGA207.016268E-444.9997022
ATAGGCG207.016268E-444.9997027
TGCCAGC207.016268E-444.9997026
CGTGATT207.016268E-444.99970232
GACGTGA207.016268E-444.99970237
GTGGGGA207.016268E-444.9997023
CGTTGGG207.016268E-444.99970236
GACAAGC207.016268E-444.99970237
CCCAGTT207.016268E-444.99970215
CGACCCA207.016268E-444.99970212
CTAAGTC253.876668E-544.99970219
GTCTACT253.876668E-544.9997027
GTCTACC207.016268E-444.99970211
TTCCGAA207.016268E-444.99970225
TACGGCT302.154482E-644.99970222
ATCACGC302.154482E-644.99970221
GCGTTAT253.876668E-544.99970235
CCGTATC207.016268E-444.99970215
Produced by FastQC (version 0.11.2)