FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_60.351000000432bd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_60.351000000432bd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120579
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC23181.9223911294669886No Hit
ACAAACATAAGTCTCAGCCCTCAAAAATTTCCTGTTATGTCTTTGATTCTT3730.3093407641463273No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT2460.20401562461125072No Hit
AGATTTGGGAGAAATGTAGAGAAAGAGTCGTTGAAAGAATTACAAAACCGG2000.16586636147256156No Hit
TCCTACAATCATACCGGATGTACAGACAAATAATCATTCCACTATGACGTC1640.13601041640750047No Hit
ATTGTTAAGAACGGAGGTCTACGCAAGGAAGAAGATTATCCTTACTCTATG1490.12357043929705835No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCG1330.11030113037925345No Hit
GAATTGAATGGAAAGGAACTCAATGGAATGGAATGGAATAGAGTGGAATGG1310.10864246676452781No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTCC302.1446722E-645.014114
GTCACCT206.997333E-445.014113
TGATGCC206.997333E-445.014114
GGGATCG206.997333E-445.014114
GTACCGA253.8628266E-545.0141071
TACCACG253.8628266E-545.01410732
CGCTCAA253.8628266E-545.01410733
ACCACGG253.8628266E-545.01410733
CCACGGA253.8628266E-545.01410734
ACTGCGA302.150813E-644.99543813
CCCTCAT207.01166E-444.99543814
GGTGTAA253.872311E-544.9954386
CACGGAC253.872311E-544.99543835
CAGCCCT207.01166E-444.99543815
CTCCCAT302.150813E-644.99543826
TGAACGC302.150813E-644.9954388
CGACCAC253.872311E-544.99543823
CTCCACA253.872311E-544.9954387
CTAAGCT207.01166E-444.99543821
ACCTCGT207.01166E-444.9954386