FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_59.351000000432a1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_59.351000000432a1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149947
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC28041.8699940645694813No Hit
ATGATAGTTTGATGGAACCCAAATAGCCAACGTAATCTTGAGAACGAAGAA4500.30010603746657155No Hit
CTTTTAATTGACACTCTTTTCCACACTTTTCATCCATAGCATGTTAAGCAT4210.2807658706076147No Hit
ACAAACATAAGTCTCAGCCCTCAAAAATTTCCTGTTATGTCTTTGATTCTT3740.24942146224999498No Hit
CAAAAGAAACAGAGAAAATCTAGTTTGACTTTTATTGGCACACAAGAGAAG3620.24141863458421978No Hit
GATTGATTGTAGGAAGAAGATCGTTTGGTAAAGGTTTGGTACAAATGCCTG3100.20673971469919372No Hit
GTACTTAAGAAGATTAAAAAACCAAAAGCAAGAAGAGTCATAGACTCAAAA3080.20540591008823117No Hit
ATATCAAAGCCAATAAACGAAGCCTTAATCATTTTCTGTTTTTTTCGTGCT3060.20407210547726864No Hit
AGTTAATTTACCCTTCATATCATAATCACATCCATAAGTGTAGTGTATGAT3050.20340520317178737No Hit
GTATGGAAATTAGAAGAGGTTTTCTTACAAAGATCAAGGGGTAATATCCCA2960.1974030824224559No Hit
ACGTTGTATGATATCATTTGAACGGAATAACATAGATCAGGATACCCTTGT2870.1914009616731245No Hit
TATTGATGTAGCATCATTTTTACTCTTGCCCGCTGTTCATATGCATTAGTA2870.1914009616731245No Hit
CCAATATACAGATCACTTTTCAGTATTAATCTCACTTAGGGTACTAGTGAG2860.19073405936764323No Hit
CTGTAAGGAACACTAAACCCATTTCCATTTTCATGTTTTCTTGAAGATGTT2790.18606574322927436No Hit
CCTTTCTAAAGCAAGGAGAATAAGAAGTAAGGGCAAGAGGCATTTCTTCTT2760.18406503631283053No Hit
GTCTACATAGTCTACATAAACATGAGTCGTATAGTTACCAATGAGATTCTT2660.17739601325801785No Hit
AGGTATAGCATGCCTTAAATGAACGATGGTTAAAAGTATTCAACTTAAGTT2640.1760622086470553No Hit
TCCTAGTAGTGTCTGTTATGTTGTTGTTTCCTAATTTCAGTTTTAGTGTCT2550.17006008789772387No Hit
GGTAAGTATCAGAATCTTCTGGCATGTCATAGTTGAAAACGATGTTCACTC2520.16805938098128007No Hit
ATGACATACCATGCATGCTGTGTGAATATGTGGAACATGAAGTTGATTCAA2520.16805938098128007No Hit
AATAACACCAACAGATTATTCGTACAACAGGAATTCAATGCACCATTTTTC2500.1667255763703175No Hit
ACTATGAAGTATGAACAATGTATTTTCACCAGATGAGACAGATACTAAGTC2460.16405796714839244No Hit
ACTGAAAACTCACTTTTACACTGTAAAAGTAGGATACTTTTACATGAAGGA2460.16405796714839244No Hit
ATATGCAACAATCTGGTTTTGATATCAGTGCTTATGCAGATGACAATAATG2450.16339106484291116No Hit
GTACTGATGATGCTTATATCTTGCAAATACAAAAGCATTAGATCAAACAAA2420.16139035792646733No Hit
GGATAAGCTTTTGACTTGTTTTGTTGTCATTGGTAAAATTGTTATTTTGCT2410.16072345562098608No Hit
ATACATAGTCTTAGTGTCTGGCTTCTTTCACTTGGCATAATGTTTTTGAGG2390.15938965101002356No Hit
GAATAGAATCGAATTGAATCAAATCGAAAAGAATCGAATGGAATCCAATCG2380.15872274870454228No Hit
ATATGTTTCTCTTGTCATATGGTTCGGACTTAAGATGCTCTCTGTTTTACT2380.15872274870454228No Hit
GAAAAGACTCATGTGTGTTATACCAAGTGATAAGACTTACAATCCAATAAC2380.15872274870454228No Hit
ACTCAGGGTTTAACCTCTATTGCATACAGATGCATGTTGATACGTTTTGTC2370.158055846399061No Hit
CATAATACCCATTTCAGTATGTCTATTTCCTCAAGTTATTAGTCCTACAAC2370.158055846399061No Hit
GTTTGAATAAATGCACGTTCTAAATCGTTCATGAATGTTTCTGCTTCAATA2360.15738894409357973No Hit
AATAGCAGGTTGGTAAAGCAAAAACCAACAATATAAGATCCCAATTGAACA2340.15605513948261718No Hit
TTCTAATGCTTAAGGAATGTTTTAAATCTCCGACATGAAGAAGATGCTTTC2300.15338753026069213No Hit
TTAGTGCCTTACCAGATATGAGATTTGCAAATATTTTCTCATTCAGTAGGT2290.15272062795521082No Hit
ATATGTTTCACTACCTGGACAATCCTAAAATACCTAAATCCACCAATGGAT2280.15205372564972958No Hit
AAATAAGCTTGTCGTTATTAGCATCCATACCTACCAGGTAACAACCGTTTA2270.1513868233442483No Hit
ATGTAAACATTCTCTTCCCTTGTCGACATCTTCGATTCAACAATCTAGAGC2240.1493861164278045No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATAG2220.14805231181684195No Hit
GAATTATAGTCCTGTGTACACAGGAAGAAAACAATAAAATGGGATTTGGTG2200.1467185072058794No Hit
GAATGGAATGGAATGGAATTGTATGGAATGGAATGGAATGGTACGGAATAG2170.1447178002894356No Hit
CTCTTAAGATTCAACAACATCACACTAAGATTAAAGTGCTCACAGAATACA2170.1447178002894356No Hit
AAGCACACTAGTCTAAAAGAGTTATACACAACACACAAAAGCACTTAGATC2160.14405089798395435No Hit
TGAACCTGATAATGGAATTGATACGGCTTATTATTCTGGACTTAGATTTGT2160.14405089798395435No Hit
TATACACAATATGCTTTCATATGCATACTACACAAATGAGCACATAGGATG2140.1427170933729918No Hit
GTATACCATACCAGAAAGAAAGGAAGATAGATGTTCTCATCAATCAATTCT2120.14138328876202924No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT2080.13871567954010416No Hit
GTATAGAACTAAGTTTAGAAAAGCTATGAACCAAGCTGATAATGATGCAAG2080.13871567954010416No Hit
AGAGATGTATGAAAACTCTTGTGTAAGAAGTCTGTTATGATCGGATATTTT2070.1380487772346229No Hit
ATGTAACATTTCGAGCAATAATATTCGGGGAGGAAAGAAATGAAAATGAAC2060.1373818749291416No Hit
TCATTGCATATGGGAACAGATAGATAAGCAGATAAATAACTGTGTCTCTAT2040.1360480703181791No Hit
TCATGTAATAGTTCCCTTGTGAGCAATTTAGAGACTTTAGAGAGCTTTCTC2020.13471426570721656No Hit
TTATTGGGTTATATCAAACAAGTGAAATGAGTTAGCATTCATTTGGTGACT2000.133380461096254No Hit
TAGTGGGTTACATTGACCAGAAGGAGAGTAATCTGGGATAAAAGAAATCCT1980.13204665648529146No Hit
GTATTATCTAGTCCCTTGGTGTATTTGACCACAATTTATCAACTAGATCCT1970.1313797541798102No Hit
GAATTATAGAGAGGCTTCATTTATGTTAAATCACATTCACATTCAAGCAAT1970.1313797541798102No Hit
AAGAGGTACATTTAGTTCCACTTTTATTAAACCTCTCATAAAACACAAACC1970.1313797541798102No Hit
AGATAAACAAATACCTATGTGGAGAAAGTATTTCCACAAACACATATCCTA1970.1313797541798102No Hit
GTACCTTTGTAAACACAGATCTGGTCATGTTATTCCATTACTCAAAAACCT1950.13004594956884766No Hit
GAATAATAGACTTACATCACGATATCTTTTTCTTCCTTATTCTGATTTTGG1920.12804524265240386No Hit
GGATGGATGGAGAAATTGACATCCTCATCTGTGATACATTTGAGCTACTGG1920.12804524265240386No Hit
CTTCTGTGGGTTTCCTAATTTTGATTTGAATGTCAGATGATTGGTATGGAA1890.12604453573596006No Hit
ATTTCACCCAAGTTACACAAGTGTGTCTAATAATAACAGAACTCAAACTCA1890.12604453573596006No Hit
AGAATTACCACAGTTATCCAAGTAAACCATACGATCAAATGAACCATAACT1880.12537763343047878No Hit
GATTAAGGTTTTATAAAGGGTCATAGTTGTTACACTTGCTCTTTCTTTCTC1880.12537763343047878No Hit
AGCAAGATTGAAATGAAGAAGGAGAGAAAGAGAGACATTAATGATCAGTTG1880.12537763343047878No Hit
ATTGAGAAGATGTGAAATGGATTAGGAACATCTTGAAAACATTATCATCTC1870.12471073112499749No Hit
AAGTATTTCTTCAGGTTTTGAACTTCTAATATGGTACTCATAATTGGTATG1860.12404382881951624No Hit
ATTAGATGATGACCCCTTTCATTTCCATTCAATGAGGATTCCATTCGGTTC1860.12404382881951624No Hit
CTTTTCGATTCGAATGTTTTCGAATCAATTCGATACTTTTTGATTCGATTC1860.12404382881951624No Hit
GTTCAAAGTAGTAACCAAGTACAACAAACAAACATCAATGTGACTCAAATT1810.12070931729210989No Hit
TAGTAGTCCTGAATATACATCAAGCCTAAGTAAATCCCTATAAGCATACCA1800.12004241498662861No Hit
GTACAATTTAAGAGTAGCAGATTAGAGAAAGACTCACAAACTAAATGATGA1790.11937551268114734No Hit
GACATAGTAGTACTCATTTGAACAGGTGGTAAACAAAAAGCGATCTAACCT1780.11870861037566607No Hit
AGAAAACACCAGTCATGGTTTTTAAGTCCATAGTGAAGCACTCAGTAAAAC1760.11737480576470352No Hit
TATTACAGATTCAGAGGACAACCCTTTAGTTTCAACATGATCAACCAATGA1760.11737480576470352No Hit
AAGTTGGTCTTTGAAAGGTGTTTACCAAAATTTGAGATATGTTTATCTTTG1760.11737480576470352No Hit
CAATATAGTTTGAAGCTCTTTCCAGATCAGTACATAGAGAGCATCCTCATT1760.11737480576470352No Hit
AATTAGTTGTAAGGATGAGTGAGAAAGAAATTGACTATGTACATCTGTCTC1760.11737480576470352No Hit
CTTTTAGTTTCTCTTCTCAAACTTACTCTTGTCATGGTTTAAGTTTCTTAC1740.11604100115374098No Hit
TTTAAGTACTTTGTGTCTTGTTATGTTTTTGAGTCTATGACTCTTCTTGCT1720.11470719654277846No Hit
TTCTAGAACATAGGATTTAGGAGCTGAATTTTTAGGAGGAAGTGTAACTGA1720.11470719654277846No Hit
TGAGAAGATCATATGAAATAGAACACGACTCCTTTTTATTCCTACTGAAAA1710.11404029423729717No Hit
GATTAAAAAACCAAAAGCAAGAAGAGTCATAGACTCAAAAACATAACAAGA1690.11270648962633464No Hit
ACTCCATACAGATCAAACATCGGAATTGGTATTCTTCTATCATGAAACACA1690.11270648962633464No Hit
TAAATTGAAGACTCTTTTCCTGACTGATTAATAGTTACAGCTGTGCTTACC1690.11270648962633464No Hit
ATAGAGATCCTTGCGATTCGTTGTATCCGTAACATTTAACAATCTCGTTTT1680.11203958732085337No Hit
CATCCCATTCTCTCTCTCTTTCTCTAATGGTTCTTAATCTGTTTTAGCATT1660.11070578270989083No Hit
ATTATCTGGACAAGACTACACCTCATTCAACTAACAGGTGGATCGGAACAC1660.11070578270989083No Hit
ATTAAAAACGTGGTTTTACAGAAATTCTTTCATGTGTGATAAAAGTTATCG1650.11003888040440955No Hit
GTATTCATTCCCAGCTTAAGATGTGTTCATCTTCTTGGTGATGTGGTTTGT1650.11003888040440955No Hit
ACACTAGAAGATTTACGAATGTCATTTACAGAATAAAGGTGTTATGCACTT1630.10870507579344701No Hit
AGAGGAAACAAAACAGAGAAAAAATCTCAAAAATGTCCCCAAACGCAACTG1630.10870507579344701No Hit
GTAGAGCAATTAATAGTGACGTTTTTCTTAACATCTAGCAACATTTCTGAA1620.10803817348796574No Hit
ACTCAGTGTGTCATTATTATGATCGGAAATGAGTTTTTCACTCAAGCTCCA1610.10737127118248449No Hit
TATTTTGCAATGTCGACTTATGAGATCGAATAATGAATAGACTCAAGATTC1610.10737127118248449No Hit
TCCTGATATTAATGCTGTATTGGGTTTTGTTTGTGCTTTCTTTTCCCTAGC1600.1067043688770032No Hit
CAATCCAATGAAATCCAAAAGAATTGAATCGAATCGAATCGAAAAGATTCG1600.1067043688770032No Hit
CTTTGATCTTTATGAGTAATAATGGTGTCTTTTGCTTTCGGTTGTTCTTCC1600.1067043688770032No Hit
TCATATAACGAGTGTCTCTACCTTCTTCTAACCCACTGAATTTCTTTCCTA1600.1067043688770032No Hit
CATACGTAGTGAGACAATGATTTGCTTAAATCCTGTCTGTTTCAAACATAG1600.1067043688770032No Hit
ACTAAAAGCATTGATTAACAAATTCAACATAACGAGATTTGGTTCAATTCC1590.10603746657152192No Hit
ACTTGTGTCAAAACACAAACAAGAAAAGTACTAATTGCAAGAAACAGTTAT1580.10537056426604068No Hit
GAATACGATGTTGAAGAGTTTGTAGAAAAACAATTGGCAGAAGATGAATTT1580.10537056426604068No Hit
ATGTTCATCTTCTTTTTCTATTCCATCTCTGTTGACAGTTCTATTTCTGTC1580.10537056426604068No Hit
AAATAGTCCTGTCTTTTTCTTTACGCTCTAAACTATCCTTAATCATTGAAA1580.10537056426604068No Hit
GTACTTTAGCATTCCCATTTTACACCAAAAGAAGTGAAGCTAAGAACATTT1560.10403675965507814No Hit
GACTCAAATGTTCTAAGTTTCTAAGATTCTAAAGGTCCACAGGTCTAGACT1560.10403675965507814No Hit
GCATATGATTCTCTGTAGAAAACCAGAGAAAGCTTCTGAATGAATGATTGA1540.10270295504411558No Hit
AGATAACAGTAGAATCTTCCAGTGAAAATTTCCCATAGGAAGCTATTTGGA1530.10203605273863432No Hit
ATGTAAAGAGGATCAAAGAGTATATGGAGAAGGTTGAGTTAGAGCTTTCTA1530.10203605273863432No Hit
TGATCAGATAATAACGCAAACAGATTATTCGTACAACAGGAATTCAATGCA1510.10070224812767177No Hit
ATATTATAGGTCTGAACAACTTTATATGCCTTCTACCTTGATTCCATACCT1510.10070224812767177No Hit
ACACAGTACTGTGAAGCAAGTGTGATCCGAAAATGAAAAGAGAAAGTACTT1500.10003534582219051No Hit
TGGTAATTTAGTGAGAAGAGTATTTAGCAACGGTAATAGTAATACCTGTCT1500.10003534582219051No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGAG253.8680908E-545.0126845
AGTGGGT253.8680908E-545.0126842
AGTGCCT253.8680908E-545.0126843
TAAGGTT253.8680908E-545.0126844
CAGGTTG302.1486703E-645.012686
TAGACAT207.003917E-445.012685
TTCACCC302.1486703E-645.012683
GTAGACC207.003917E-445.012683
AGACCTC207.003917E-445.012685
GACCTCA207.003917E-445.012686
ATTTAGG302.1536143E-644.99766515
ATTTACG207.0154446E-444.99766511
CGTGGTT207.0154446E-444.9976659
GAGGATC207.0154446E-444.9976658
CGGAATT302.1536143E-644.99766521
GGAATGT302.1536143E-644.99766514
GGCAGTA207.0154446E-444.99766533
TACCCTA207.0154446E-444.99766539
CCTTAAG207.0154446E-444.99766536
CCCCTAT207.0154446E-444.99766533
Produced by FastQC (version 0.11.2)