FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_57.3510000004326d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_57.3510000004326d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57635
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC37916.577600416413637No Hit
GTTTATTCTTATGATGGCAGATGTAGCTCTATTTTTACATGGTTAAGATGG7361.2770018218096642No Hit
GGTTTTGATTTAGGGTTAGGATTGCACCAAAAATAAGATCCACTTTACCAC7291.2648564240478877No Hit
GACTAGTGAAATCAAGAGCAAGTTTAGGATTGGATGTTTTGAGAAGAGCTA6881.1937190943003384No Hit
ATGTTCATTCTCTCTTCCAATAGATAATCCAATATCTTCAGGATGTGATTT5831.011538127873688No Hit
AACCAATATACTTGTGCTCTTTTAGGTTTTTTTGCTAAAATAGACTTGATG5270.9143749457794743No Hit
GTACATAGTTGATTCTTTTTCAGTTTTGTGTTTGGTATGGAAGATAACAGT4780.8293571614470373No Hit
ATATATGTCCTTACCAAGTTCTTTTTAGTTTTTCATTCTGTCATTCGTTTT4650.8068014227465949No Hit
CTACATTCACTGTAAATTCACCTGTACTGTTACCATTACAAAGAATTGTTT4230.7339290361759347No Hit
CTCTTTAGCTTAATTAGATACCATTTGACAATTTTGGCTTTTGTTGCAATT3860.6697319337208294No Hit
AACTCAGACATTTGGAGATATGTTGTATTACCATGATTCTCAAGGTTGTCA3810.6610566496052745No Hit
ATCAAATGGAGAAATACAATCCGATACAACAGATGACTCTATGTCTGATCA3640.6315606836123884No Hit
ATATAATCTCCAGACGATGATGAATCTAGAAGATGAAGAAACACACATCTT3500.6072698880888349No Hit
GATTAGACTTATTAGGCCATGGTTATTTCAAAACGTTTAACGTATACAAAA3470.602064717619502No Hit
GTTGGTCTCTTAACAGAAGATCGTTTAAGAAGATACCAAACATAGTCCAAG3140.5448078424568406No Hit
ACATAAAACAGAGTCCTCACTTTCATTTCTCTCCAAGATAAACTTTAAGGT3060.5309273878719528No Hit
GTGTTTCAACAGTTTTTAAATGCAGACTTACAAATTATGGTAGGATCGGCA3060.5309273878719528No Hit
GTTGTTTCCTGACTTTTTAATGATCTCCATTCTAACTGGTGTGAGATGATA2670.46326017177062545No Hit
AATGAATTCCACAGGTATGATGTGAAGATGAAAAAGTGGTGAAGGATAAAC2560.444174546716405No Hit
ATTATTATCATTCCACTGGAAGTACAAACTCATCACATGGCAGAGTTGCTA2500.4337642057777392No Hit
AACTTGGAATCCTAATCTTGTCATTCCTGTATCGATTTTGATGTGAACCTT2420.41988375119285154No Hit
ATGCTTGGAAGTTAAAATTGCAACTACACACATTGCTTGCAATTTTATGTT2410.41814869436974056No Hit
GGAACATAAACAACAGAACATAACAAAGCCCAAACAATCTTAGTAATAATC2270.3938578988461872No Hit
AAATACTAATGATGATGACGATGATCCTTGTTTTGTTCCTGACTTGAATGA2240.38865272837685433No Hit
AGATAAAGTTATGGAAGTAAAACTGATCAATAGCAAAATCCTGTTGAAAGC2180.3782423874381886No Hit
GTATAATGCGTTTAGATTTTCAGGGTCTGTTTTTAATACTTGTTCAAAGTA2130.3695671033226338No Hit
GGTGATATGTATGACCTTTCTAGTCTTTTCCTTCAAATCTGTATTGTATTT2030.35221653509152423No Hit
TTTGAAAAGAGAGTGACCTCTTGAAATTTGCTAGGAATTACAGCATCAACA1890.32792573956797083No Hit
AACGTAAAGAAGAAGAAGAATCATCATCTCTGGTTTGATATCAACAAAACC1880.3261906827448599No Hit
TATCAATACGGCTTATGACAGAATAGTTAAGTCTGATGTTAAATATCGTTT1870.3244556259217489No Hit
AGTCAATAGTTAGAATAATCCTAATGACTACTGACTAATGACCATTGACCT1830.3175153986293051No Hit
ACCCAATTATTGCAATCTTCGTCTCCTTTTGTGTGTTCATTGGTACAAACT1810.31404528498308315No Hit
ACATATACTTGGACAAGCAATATATCATTTTCAGTAGTTTTTGTGAAAAGG1800.3123102281599722No Hit
CATTTATTTCAACATGCTTGACAAAATAAGAGCTCAAAATCTATCTCTGAT1730.3001648303981955No Hit
ATAGTAACTATGTTCCTTCTCTGAAACTGGATATTGTGGTTCGTTCTCTCG1720.2984297735750846No Hit
ATCTATACTTGAACCAAACATCTAATGCACCTCCTCAAATTGAATGTGATG1500.2602585234666435No Hit
CCTCAACATAATAAAGACCACATATGACAAACTCATAACAAACATCATATT1470.25505335299731063No Hit
GTCAATCCATTTGATCCTTGTTTTATGAATTCTTAACCAGAATGCAATCTA1470.25505335299731063No Hit
TATCACTTCTAAATGGGAGAATGAATGGGAAAACTCTTGGAAAATTTTGAG1440.2498481825279778No Hit
ATTTGTAGGGTCTTATAAATTAAGCTTTCTCAACTACTTCAAGCCAAGTAA1410.24464301205864492No Hit
CATATAACTGCTGTCTTTCTTGCTTTCTGTTTTGATACAAACATTCTACAA1340.23249761429686824No Hit
GAATAGAACGGAATTGTCATCGAATGGAATCGAATGGAATCATCTAATGGA1330.2307625574737573No Hit
ATTTAAAGTTTGCTTGTGTCCATTTGGACCATCTTCCTTATATTTGTTGGT1330.2307625574737573No Hit
TTCCGATATTGACGCAAAACTTGAAGATGTGGAAAGAATCGAAAAGAAAAT1330.2307625574737573No Hit
CACTTTAACCACGTAATGTGAATGTTCAATGTAAATTTCAGCATTTGTTTT1320.22902750065064628No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1320.22902750065064628No Hit
GGTTATAATGAGAAAACTTTTCATATACGAGTTGACAGATGATAATAGCAC1280.2220872733582025No Hit
GAATAGAATGTTACAAAGCTTTGATAAGTTCTTTGCATATAAGTAACTAGT1260.21861715971198056No Hit
GTGTTACTTGCATTTATTTTTATGTCGTCTTTGTTTGTTCCACTGTATTGC1240.21514704606575866No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGT1180.20473670512709288No Hit
CAAATCAGCAAAACCAAAAAGTGTATGTGCATCTTCCTTGATATGCAAAAA1180.20473670512709288No Hit
AACAATGAGATCCCATCTCTTGATTTTGTTAATCTCTCTCTTAATCTTGAA1140.1977964778346491No Hit
ATGTATTATAGGCTAGGAATACAGCAGTAGACAAAAATGATAAACCGTGTT1140.1977964778346491No Hit
GATCATAACATCAATATACATTTCTCTTCAAGTTCTTGTAACACAAATGTA1130.19606142101153812No Hit
CATTTATATCATCTAGAACCTGACAACTAATTTTTCCCTATGTAACTCTTG1090.1891211937190943No Hit
GTATAGAACCAACCATTGTTCAAAATAGTACTAACCAAAAAACACCACAAA1070.18565108007287237No Hit
GGGAGGAGAAGATATCAAATTTATTGCACGAAGAATGTTGATATTAGCCAG1070.18565108007287237No Hit
ATCTTATATTCTCAGGTCTTCTATACTATACTGTGTGTTCTTTCAGGAAAA1070.18565108007287237No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1060.18391602324976142No Hit
GAAAAGCCAATAGAATGTGCTGGTTAACTTGATGATCAATAAAGTTTCAAA1000.17350568231109567No Hit
GTTTCAATATGATCTGTAAATTACAGCTTTCACTTTGCTTGTATATTTTCC990.17177062548798472No Hit
ATTTTGTCATCATCCAAACAAAATTTTTGTTGAGAGGTTCTGTCAACAGAA990.17177062548798472No Hit
GGAATAGAATGGAATGGAATGGAATCAACTCGAGTGGAATGAAATGTAATG980.1700355686648738No Hit
GTTTTAAAGATGAAGATTTTGAGTTCCAAAAATTAGGAGACTTACCCAAGA980.1700355686648738No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA950.1648303981955409No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA940.16309534137242995No Hit
TAGTAGCATGTTAGAGTTAAGCATAGTTAGAATAGTCTCCTGAAAGGTAAG940.16309534137242995No Hit
ATTAGCATCTTTCTTTCTCTCTGCCCCAAAAACTCCACATTCATTTACATA930.161360284549319No Hit
CATTGTAAAAAAAACTGTGGTTGAAGAACATAGAAGAAGAGGTTGCTGTGG920.15962522772620802No Hit
GTATTTAGAGGAAGCATTTCTTACAACAACACACAAAAAGCAAAAGATAAA910.1578901709030971No Hit
GAAAATAGAGTTTTGTTGCCAAGAATCAAGAACAAACAAAGTATGTCTCTC910.1578901709030971No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA880.1526850004337642No Hit
ATACTACTACTACCTGTAATCTTTTTCCCTATCTTTTTTTTGGTCGTATTT860.1492148867875423No Hit
GGATGTTTTAACACTGAAAACAAAGGTAGAAAGAATCAACTTTTAAACTGA800.13880454584887655No Hit
AAATCATTGAATGGACTCAAAAGGAATCATCATCGAATGGTATTGAACGGA790.1370694890257656No Hit
GTACAATACAATTGCACAAAAGCCTTCCAGAGATATTACAACTCCAACAAC790.1370694890257656No Hit
ATTTTTTAACTCAAAATTGTGATTAAGGCTCACAACAGGTTCAAAAATGAG790.1370694890257656No Hit
CTTATGTTGATTGGACGGATAGTTTATTGAAAAAATGGGTGGCTGATGATA760.1318643185564327No Hit
AGATAAAGGTTTCTAAGTGAACTTATTATTTTGACTTCACTTGATCGATAT750.13012926173332176No Hit
ATACATATGAATGCATATGTGGCCATCTGGAAAAAACTAGTAGAAATCACA740.1283942049102108No Hit
CAATGATATCGTCTCTGCAGATATATGAATCTGTACAGAAAAGATCAGCCA730.12665914808709985No Hit
TTCGTATATTCATGCATAAACCTGCAAATGAATCAGTATTTATCACGATTG720.1249240912639889No Hit
GAAATGATGATGAAGACGAAGAGAAAGAAACAATGGACATAGATCTCTAGT710.12318903444087793No Hit
ATTTAAGAAAATGGGCTTTGATTGTGTTTATCCACCAAATACCCCAATCGA710.12318903444087793No Hit
ACCATGAATGATATGTAAAGAGAAATACTCATTGGTTGGACATAACAATAA710.12318903444087793No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCG710.12318903444087793No Hit
ACTTTATTCATTTAAATGGAGATGCAACAAAACTCAACTATGAAACAGATG700.12145397761776698No Hit
CCTTTGAATTCCATTTGATGACAATTCCATTCAATACCAATTGATGATGTT700.12145397761776698No Hit
CAAATATACTAATCCTACTTTGTCTAGTTTGTGTTTTGTGGTGATTTTATT690.11971892079465603No Hit
AGATATTATGATTCCAGTCATGAACGGAAACTTTTTCACTGAATAATGAAG690.11971892079465603No Hit
GGATAGTTTTGCTGGGTATAATATTCTTGGCTGATAGTTTTTTTTCTTCTC680.11798386397154506No Hit
TCTTTAAGATGCATCATTAGATTGCTTATTTGAAGTTTTTCTTTCTTTCTT680.11798386397154506No Hit
ATTATTAGGCAATTTTCCTAACTCTACTTCTACAAGAGTTTTCCTCAATTA680.11798386397154506No Hit
GATCATAAGATTAAACAGAGAGATTAGAGATGGTTTTTAATATCATCCTCA670.11624880714843412No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.11624880714843412No Hit
CATGTTGTTACCTAGAACGAGTAGGAAATCTCATTGATGGTTGTGATCTTT660.11451375032532314No Hit
TTTTGAAAGAGCAAATTGAAGTGAATGTTGACAAGCTTGAAATTGGTGAAA650.1127786935022122No Hit
GTTATATTGTGTCTAACAAGCAATTAGCATTCAAAGGTATTTGACTCTTGC640.11104363667910125No Hit
ATCTTAGACTTGTACATTGGTGAAGAACCGTTTGATAAGAATGCAAGAGAA640.11104363667910125No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.11104363667910125No Hit
CATTACATTAAGAGGTACATTTAGTTCCACTTTTATTAAACCTCTCATAAA640.11104363667910125No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA640.11104363667910125No Hit
ATATTCAGGTTGAACTATGCCTGAACAGAAATCAGATTGTTGTTAGCATAC640.11104363667910125No Hit
CGATGATATCATTCGATTCCATTTGATGATAATTCCATTTGATTCCATGCG640.11104363667910125No Hit
GAGATATTGGATCATGAGTGAGTGAAAAGAGCTGATATTACCGATTTTTAA630.10930857985599028No Hit
ATCTTCACTTATCTTTTTACTTTGCTTTGCCAGTTCAAAAACTTTTTCTTG630.10930857985599028No Hit
CCAATAAAATGGGAAGGAAAAATCAAGAAGTGGAGTTTAAGAAAGAAGTGA620.10757352303287933No Hit
GAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.10757352303287933No Hit
GTACAGAAGAAATGACAGAGAATGCTTCTTCTGTCTTTCCATTTTTCTTCT620.10757352303287933No Hit
ACGATGAGTTGATTGAACTTTCTGAAGCGTTGAAAAGACAAATAGACAATT610.10583846620976836No Hit
AACAAGAAGAAGAAGAACAAGACAAGAAGAACAAAGTACCCAAGAAAAACA610.10583846620976836No Hit
GGAATGACGATTATCAATGGAGTCTTCACGAAAGATGGGTTTGAGAAGTGT610.10583846620976836No Hit
GAGTAGTAGTAATCTTCTTTTTCTTCATCTTCTCTTCTACTTCTTCTCTCT600.10410340938665742No Hit
GAAGACGAGGATGAAGAAGAGATATTGGATCATGAGTGAGTGAAAAGAGCT600.10410340938665742No Hit
CTATGATTGTTAACAAGTATAAGCTTAGAGGGAATGTTAAGAGTTTTAACC600.10410340938665742No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.10410340938665742No Hit
GAGTTTTATCATCTTTTGTTGTCTTTTGGATTTTGGAAAGAGCGAGATCTC590.10236835256354644No Hit
CTTTTAGACACAATGTGATTTGTGTGTAACATTAGCTTTAATAGCAGAAAA590.10236835256354644No Hit
GGACAAAAGAAAAAGTACAAGATACCCAAAAAAAGAGTGTAGAAACTACAT590.10236835256354644No Hit
TGTATACATTTTTAACTAACCTGTACATTGTGTACATGTACCCTAAAACTT580.1006332957404355No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA580.1006332957404355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTTA600.045.00000426
CTTTCAT351.1852717E-745.00000420
CTCTGAA351.1852717E-745.00000419
GGTTGTC351.1852717E-745.00000444
CATGATT351.1852717E-745.00000432
ACTGTAA600.045.0000049
AAACTGG351.1852717E-745.00000424
TCCTCAC351.1852717E-745.00000414
AATCCGA302.1296728E-645.00000418
TAAATTC600.045.00000413
TCAAGGT351.1852717E-745.00000440
TTCGTTC351.1852717E-745.00000440
ACAGAGT351.1852717E-745.0000048
CTTTAAG351.1852717E-745.00000443
ACATGGC351.1852717E-745.00000435
TTGTAGG302.1296728E-645.0000043
ACCATTA600.045.00000432
AACTGGA351.1852717E-745.00000425
ACCATGA351.1852717E-745.00000430
TATGTTC351.1852717E-745.0000049
Produced by FastQC (version 0.11.2)