Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l01n01_bir_1228_pcontrolb_56.35100000043244.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 48552 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 46 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC | 10146 | 20.897182402372714 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG | 3487 | 7.181990443236118 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT | 2847 | 5.863816114681167 | No Hit |
GTCTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGC | 123 | 0.25333662876915475 | No Hit |
CATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTC | 91 | 0.18742791234140715 | No Hit |
TCTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCC | 56 | 0.11534025374855825 | No Hit |
GATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTC | 53 | 0.1091613115834569 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AGAACAT | 25 | 3.8356906E-5 | 45.0 | 21 |
GCCGTCT | 20 | 6.9666625E-4 | 45.0 | 45 |
TGCCGTC | 1210 | 0.0 | 44.07025 | 45 |
AACGCAG | 25 | 0.002087335 | 36.0 | 6 |
TGTGCAG | 25 | 0.002087335 | 36.0 | 22 |
ATGCCGT | 1505 | 0.0 | 35.431892 | 44 |
ACGCAGA | 30 | 0.0050994093 | 30.000002 | 7 |
CGCAGAG | 30 | 0.0050994093 | 30.000002 | 8 |
CAACGCA | 30 | 0.0050994093 | 30.000002 | 5 |
TATGCCG | 1860 | 0.0 | 28.548388 | 43 |
GTATGCC | 1865 | 0.0 | 28.47185 | 42 |
TCGTATG | 1870 | 0.0 | 28.155083 | 40 |
CTCGTAT | 1870 | 0.0 | 28.034761 | 39 |
CGTATGC | 1880 | 0.0 | 28.00532 | 41 |
TCTCGTA | 1875 | 0.0 | 27.84 | 38 |
TATCTCG | 1870 | 0.0 | 27.794119 | 36 |
ATCTCGT | 1875 | 0.0 | 27.720001 | 37 |
AACCTAT | 1885 | 0.0 | 27.692307 | 32 |
TAACCTA | 1895 | 0.0 | 27.664907 | 31 |
ACCTATC | 1890 | 0.0 | 27.619045 | 33 |