FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_56.35100000043244.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_56.35100000043244.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48552
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC1014620.897182402372714No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG34877.181990443236118No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT28475.863816114681167No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGC1230.25333662876915475No Hit
CATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTC910.18742791234140715No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCC560.11534025374855825No Hit
GATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTC530.1091613115834569No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAACAT253.8356906E-545.021
GCCGTCT206.9666625E-445.045
TGCCGTC12100.044.0702545
AACGCAG250.00208733536.06
TGTGCAG250.00208733536.022
ATGCCGT15050.035.43189244
ACGCAGA300.005099409330.0000027
CGCAGAG300.005099409330.0000028
CAACGCA300.005099409330.0000025
TATGCCG18600.028.54838843
GTATGCC18650.028.4718542
TCGTATG18700.028.15508340
CTCGTAT18700.028.03476139
CGTATGC18800.028.0053241
TCTCGTA18750.027.8438
TATCTCG18700.027.79411936
ATCTCGT18750.027.72000137
AACCTAT18850.027.69230732
TAACCTA18950.027.66490731
ACCTATC18900.027.61904533