FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_55.3510000004322a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_55.3510000004322a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190580
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC98165.1505929268548645No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT24051.2619372442019101No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG9600.5037254696190576No Hit
GTGTAATGTTAGTGTTTTGCTGTAGATGCTAGTTACTAACTTTGTATGACG4270.22405289117431001No Hit
ATTGAAGTTTGTAAAGTCATTAAATGTCCTATCACTGTCTCTTATACACAT3860.20253961590932942No Hit
ACTTATTTCACCTTCTTTTAGTAACCCATCACTGGTAATATTCAAGCTGTC3840.20149018784762304No Hit
TCATTAAATCAGTTATGGTTCATTTGATCGTATGGTTTACTTCTGTCTCTT3530.18522405289117433No Hit
TTTCGATTCAATTCGATTCTTTTCGATTCCATTAAATTTGATTCGATTCTT3520.18469933886032114No Hit
ATATAGTGTACCTTGATGAAACTAGCTATTCATCAAGGTTTATGGAAACAG3330.17472977227411063No Hit
CAATAAATCCATAAAAGTCAACCTCTATGGTTTTAAGGGTAAGAAATGTGT3320.1742050582432574No Hit
GTACAAATTATCTGCTTAAGAATACACTCTGAAATGTGGTCAGTGATTTTT3270.1715814880889915No Hit
TACTGTCACACTAAGTAGGATGTGATAAGTTACATGTCTGGTAATTCAGTG3260.17105677405813832No Hit
CTAACTAATACTGGCTGTCAAAAGAATACATGAGGTTTTGAAAAGATGAGC3220.16895791793472556No Hit
GGGTATTGGATACTTCCTATAATAGCTTCGTTTGCATCGTTTACTTTGAAA3190.16738377584216602No Hit
ATCCAATACTGATTGTAGGTAGCATTTTGATTAAACTGCTGATTTTCGTAG3180.16685906181131283No Hit
AGTAACTAGTAGCTAGTAGCTAGAAACTATTTTCCCAAAGCCTTCTTTATC3090.16213663553363417No Hit
AACATCATCAGCTCTTTTAACCTGTTAACCAGATAGTTACAGAAGTCTTCA3090.16213663553363417No Hit
GTATCATTCTCAGTAGTAAATACAAGCTCATTAACATACTTCTTAGGCTGT3080.16161192150278098No Hit
GTTATTAGCATTGGTTATGGTGATATCTCAGAGAGTTAAGCTGTTAATCTG3070.1610872074719278No Hit
ACTGTATGGTACAAAAGAGACATGTTTGTGATGTTTGACAACAACATTCCA3060.16056249344107462No Hit
TTCTACCATAACTGTACCATTCATGTTTTCAAGAAGCTTGTCTTAAGATAT3050.16003777941022143No Hit
ATTGAAAACCATTTATGCCCGTATGTAGAAGAACTATTACTCCTGTCTCTT3050.16003777941022143No Hit
GAAGAAAAAGAAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAACTGTCTCT3030.15898835134851505No Hit
GGTGTTTAACCAATGTAATACCTGACTGTGTATTAGTACTGTCTCTTATAC2980.15636478119424913No Hit
TATCCATACTGGTTCAAACATAATCACTCTATGCTAGGCACTATATAATTG2970.15584006716339596No Hit
GAATAGAATCAAAAAGAATCGAATCGAATCAAATCGAAAACAATCAAATTG2970.15584006716339596No Hit
GAATCGAATTGATTCGAAAAGAATCGAAATGAAACAAATCAGATCAAATGG2960.15531535313254277No Hit
ATTTTCAACTAGACTAGCCTTTATGCAGAAGAGAAATGAACATATTCTGTC2920.15321649700913No Hit
ATCTTAAGAATAATCATGACTCAGCTACACTATTCTTCCACTTTCTCATCT2860.15006821282401092No Hit
ATACAGCTAACAAATTGCTTAGAGGGAAATTTATAGCTGTAAACACCTGTT2810.147444642669745No Hit
GTTAGTAACACATTCAATTATTGTTGTAAGTCCTAAGAGTTGTTTGTGTAT2780.14587050057718542No Hit
ATACGATGAATAACACTCGCATGAATTTTCAAACTTCTTTCAGATGACATA2770.14534578654633226No Hit
GTATTAATGACCCTCTCTTTATTGACAAATCCTTTTTGTGCAGTAGCAACA2710.14219750236121315No Hit
ACTCGATTCTTTTCGATTCAATTCTTTTCGATTCAATGAAATACGATTCTA2690.14114807429950676No Hit
TATGTGTTCATCAAGGACAATTGCATGCACTTACAGAGGTAAGACATCAGT2620.13747507608353446No Hit
GAATCGAAAAGAATCGAAATGAAACAAATCAGATCAAATGGAAAAGCATCA2610.1369503620526813No Hit
CTTTTTAACACTCATTCTTCACATGTCTATAGTATGGCACTAAAACACTCA2550.13380207786756218No Hit
CTTTGAAAACATCATGCTAAGTTACATAAGTGAGACACAAAAGGACAGATA2530.1327526498058558No Hit
ATACTCTTCCATTGTTTCTTTCTGAAAAACCAGACCAAATGTTGATATTTC2510.13170322174414945No Hit
ATGCATAACATTAAAGCCTATTGCAGCAATTAATCCACCTAAAATGATACA2500.13117850771329626No Hit
TTCTAACTGGAAATGTAGTTTTTCTACTAGGAATACCTGTCTCTTATACAC2490.13065379368244306No Hit
AATTGATGCAAGCTAAAGACGAATATATTGAAAACCTGCAAAATGTATTAA2460.12907965158988352No Hit
AGAGGAAACAGATAACCTAAAGAATGGGAGAAAATATTCCTGTCTCTTATA2430.12750550949732395No Hit
ACCATTAATTGCTACTTTTACTGCCATTATAATCTGTCTCTTATACACATC2400.1259313674047644No Hit
AGTTAGGATGGCTATTATCAGAAAGAAGAAAAACAAATGTTGGTGAGGTTA2370.12435722531220486No Hit
TGATTATTAAATGCCTTGAGATAGTCTTCTTTCTGTTAAAACTGCGTGTCA2350.12330779725049847No Hit
GCATAGTATTCCATGGTGTCTATGTACCACATTTTCTTTATCCAGTCTATC2340.12278308321964529No Hit
CTATTAGATACTGTTGTTACACGCGTTGAAAAAGGTAAAGAAGTAATTGAT2330.1222583691887921No Hit
ACTTTATGCTAAGTGAAATAAACTAGTCACAGAAGGACAACTACTACATGA2330.1222583691887921No Hit
TTACTACACAATCTGTTAAATTTGACGATCTAAGTACCTGTCTCTTATACA2320.12173365515793892No Hit
AAACAATTCCGTATTCGATACGATTATTTTTTTAGTCGACCATCTATACCT2300.12068422709623255No Hit
GGTGATAACATGTACATGAAATTGGGGATCAATAAAATTCCTGAGTTGTTA2300.12068422709623255No Hit
CATTAGGGTTAGAAAGAATATCAATATCAATCACCGAAAGAAGCTAACAGT2290.12015951306537938No Hit
GTAGTGTTTTTACTCTTCCTCCTAAAATAGATGGATAACCAGTTAAGCTTT2280.11963479903452619No Hit
TATTTCAACAGTAGTAGTGGTTGCTTCTTCTTCATCAGTAAGTGTGGGTTT2280.11963479903452619No Hit
ATTGTAATTGTCAGACCGAAGACGATGTGATAAACACATTAAAAGATATCG2270.11911008500367301No Hit
GAATGTAAAAGTTCATCAAAGTACTTGTCACATAAATGTGAGTGTACTACT2260.1185853709728198No Hit
ATTACGTACAAGTGTCCTAGTGCTCATGATGGTAAATTTAAATCCGTGTTG2250.11806065694196662No Hit
AAGCAACAAGGAAATTTTTGTACACGTTTCTGGCTTAAAAGAACAAATTAC2210.1159618008185539No Hit
TTTTTACTCCTTTCACTGAATGTCTTACACTAAATCCACAGTATCTGTAAG2180.11438765872599434No Hit
ATGTTAGATGGAAGAAAGTATCAATCCAACCATCAATATTGGTCCTGTTAC2170.11386294469514116No Hit
TCATATCTACATCTTGAACGAACTTACCATCTCTAAGAAATACCTGTCTCT2150.11281351663343477No Hit
GATCTATACAATGTAGATGGAAAAGATATCATCATTTTAGCTGAAGGTCTG2140.11228880260258159No Hit
AATATGGGTATAGCAATTTTGAAAACGGAGATCATTCAAAAAATACATCTC2130.11176408857172841No Hit
CATAAATTTAGTGGTGAGTTATTCACATGCATGTTTTTAAACTGTCTCTTA2120.11123937454087522No Hit
GTGTAATGGTAATGTGGTAAATGTTCCATTGATATTTTGAGTGCACATAGC2110.11071466051002204No Hit
TAATAGCCTCATTGAGTTGTAGTTCTCTATTTCCAATATTGCTCCCTAATC2110.11071466051002204No Hit
GAATTAATGTTACCATACCTTTTATCACTGATCTTTGTCAGTCACTCTAAG2100.11018994647916885No Hit
ACGAAAGATGTCATGATATCTTAACAACGCATCAAGAACAATTAACATTAA2070.10861580438660928No Hit
AAGTACTATGATAGTTAGCTCTTCTCTTCCTTTTCTTTATCTGTCTCTGTC2070.10861580438660928No Hit
TTGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAAACCATACGA2050.10756637632490293No Hit
CTGTATTTCTCAGGTTAAAAAAAAGACACAAAAGTTCTGGTGACATAAAGA2040.10704166229404974No Hit
ACTGCATAATATCCACCTAAATACTGACTTTGACTGGATAAATTTGGATCA2030.10651694826319656No Hit
TCCTTACCAACACTTGTTATTTCTTGTATTGTTGATTTTAGCCATTCTGAC2030.10651694826319656No Hit
GCTTATTTCCTACTATGTCCTCACATTATTCTCATTTCCCTTAGTACTGTC2020.10599223423234337No Hit
GTTCTAACTTGAAATTAACACATTGCTAAAGCTAGAAAACTGAATATTCGT2010.1054675202014902No Hit
CTGTATTTTTATGAATCTGATGAAGCTTCCTTATAGAACTGTCTCTTATAC2010.1054675202014902No Hit
CATCGTATTGTTAAGTCAATTAATCAGAAAGCTCCTGATATTACACCTGTC1990.10441809213978383No Hit
GAAAATAGTTTGTCACTGATGTTAAAAATGGACACAAGATGACATTCTAGA1980.10389337810893064No Hit
ATGTCAATGCATGCTTTATCTACCTTACATAGTTTATGTGCTGTCTCTTAT1980.10389337810893064No Hit
ATACAAAGTCGTATATTTGCTGATTACTCTATTGCATCTTTTACTACTGGT1950.10231923601637108No Hit
TTGTTGAATTCATCCCAGAGTACAAGATCATTGTGTTACTGATGATTAACA1950.10231923601637108No Hit
ATCATTTACTTCGGTTTCAAACAACCAGAAAATGATATCACTTTTAGATCT1940.10179452198551789No Hit
GTATCTTATGGTTTTAGGTGCAATTGTAAATGGGATTGACTAATTGAGTTC1940.10179452198551789No Hit
CTATATGTTGTGTGCATATGCAAATCTCCATTGATATCATCTAATTCTGTC1940.10179452198551789No Hit
GGAAAAAAGGGAGACTGTTATGACCGATATTTGATTAGAGTTGAGGAAATG1930.10126980795466471No Hit
CTACTAGGTAGTAGTACCTACTACTACTAGTAGTAGTAGGTCTGTCTCTTA1920.10074509392381152No Hit
ACATTACTGAACCTGAAAAGAAAACTGCATATTGGAGAGACATAAATGCTC1910.10022037989295834No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAG253.8631784E-545.03022
CTCCCAA206.9952925E-445.03026
CCAGAAC206.9952925E-445.03023
TACGAGC206.9952925E-445.03026
GCATAAC453.783498E-1045.0301973
GGGGCAG207.0224964E-444.9947526
TCGCCTA253.8811966E-544.9947511
CTGCTCA351.2059172E-744.9947510
GTTTGGC253.8811966E-544.9947523
AGCGTTC207.0224964E-444.9947512
TTCTAAG207.0224964E-444.9947533
AGCGTGT302.157678E-644.994758
AGCGTAA207.0224964E-444.9947519
CGTGGAA253.8811966E-544.9947534
GGGGAGG253.8811966E-544.9947525
GACAGGT253.8811966E-544.994759
GGTACTC207.0224964E-444.9947536
TCGTTCG253.8811966E-544.994759
TCGTTAA253.8811966E-544.9947520
CCCCTCT207.0224964E-444.9947532
Produced by FastQC (version 0.11.2)