FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_54.35100000043200.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_54.35100000043200.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194097
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC165548.528725328057622No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT41442.1350149667434324No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG10790.5559076132037074No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC3050.15713792588241962No Hit
GAATGTAATGGAATGCAATGGAATCAAATGAACACGAATGGAATGGAATGT2330.12004307124788122No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC2280.11746703967603826No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTG302.1561518E-645.00000431
TGCCCAC302.1561518E-645.00000431
TCGGAGG302.1561518E-645.00000418
CATAGTG302.1561518E-645.00000439
TTGAACG302.1561518E-645.00000417
CGGAGGA302.1561518E-645.00000419
CCACAAC207.018785E-445.08
GTCCTAC207.018785E-445.040
GACGTGA207.018785E-445.027
GGTCCTA207.018785E-445.039
CTCCCCT207.018785E-445.010
AGGGTAG253.8787897E-545.033
GACCCCT207.018785E-445.034
TTCCCCA207.018785E-445.029
ACGTGAT351.2049532E-745.020
AGACAGC207.018785E-445.045
ATGACCG253.8787897E-545.016
TCCCGAC253.8787897E-545.043
GTCCCAC207.018785E-445.09
TCCCCAT253.8787897E-545.023