FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_53.351000000431e5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_53.351000000431e5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74803
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC1586321.206368728526932No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT907912.137213748111707No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG44255.915538146865767No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTAT1770.2366215258746307No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGC1560.20854778551662365No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC1440.19250564816919108No Hit
GCATTGAGTAAATGTTCACTGTCTCTTATACACATCTCCGAGCCCACGAGA1140.1524003048006096No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC1110.14838977046375143No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCC950.12700025400050802No Hit
GGCATTGAGTAAATGTTCACTGTCTCTTATACACATCTCCGAGCCCACGAG860.11496865098993357No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA810.10828442709516997No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTAAG206.971065E-445.0260852
TGCCGTC17800.044.49041745
CTGATTA250.002088607536.020874
ACTCGTT250.002095482335.9967923
GCCCATT250.002095482335.9967937
GCTGCAG250.002095482335.996798
CCACCCG352.7968682E-432.1399922
TGCAACA300.005119138429.99732616
TGCTGCA300.005119138429.9973267
CCCATTT300.005119138429.99732638
ATGCCGT26600.029.77178444
AAAAAGT1550.027.5966321
AAAAGTA1700.026.4859332
TATGCCG30950.025.51468843
GTATGCC30950.025.36930742
CGTATGC31300.025.08562341
TCGTATG31300.025.01374440
CACCCGT450.001216495124.9977723
CTCGTAT31450.024.96597939
TCTCGTA31550.024.81553838
Produced by FastQC (version 0.11.2)