H23LNBCXX_l01n01_bir_1228_pcontrolb_52.351000000431cb.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l01n01_bir_1228_pcontrolb_52.351000000431cb.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	79222
Sequences flagged as poor quality	0
Sequence length	51
%GC	36

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC	3914	4.940546817803136	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT	479	0.6046300270126985	No Hit
CTTAATCCTTTGACACATCTACAAAATCACATAACGTTTGACCAAGTTTCA	194	0.2448814723182954	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG	174	0.21963595970816185	No Hit
TAATTGAGTCCATTAAGTACTCCATGGACAATGTTTAAGCTGTACTTCCTC	144	0.18176769079296154	No Hit
AAACTCTTCTATCTGCAAAATGTAGGAAACCAATGTTAAATTTAGTGTCAA	132	0.1666203832268814	No Hit
TTACTACACTTATCTAAGTTACAGAGGAAACTAGCTTTCTTGTATGAAATG	128	0.16157128070485471	No Hit
AGTATATTGTGTGATGTCATAATGTCAATAAGCTTCACGCACCAATGAACA	125	0.15778445381333467	No Hit
TCATAACGGTATCGGAAATTCTCATAAGTCGTCTGGTTAGTACAGATGAGA	121	0.15273535129130797	No Hit
TTGTTATGTGATCTTTAGGATAATTCCCTTAGTTTCCATCACCAAAACCAT	120	0.1514730756608013	No Hit
ATATACATCCATACACAATGGAATATTACTCAGCCATCAGAAAGAATGAAT	115	0.1451616975082679	No Hit
ACTCCAATCTGTTAAAATGCATGTTGAATGTTGTTACTTGCAATTACCTCT	115	0.1451616975082679	No Hit
ATCATCTTCTTCTTCATCTTATTCTTCTTCATCTTCTTCTTCTTCCACCTC	115	0.1451616975082679	No Hit
GAATTACAAAAAGCCGTATTCCCATTGATTACAGGATTAAGAAATACTGGC	109	0.13758804372522784	No Hit
GATAAGAGTGTTATGTATGTCCCATGATACTTAATTCTCTTATCCACCCTA	108	0.13632576809472116	No Hit
AGGATACGCATAACGTTGTTTGTACAATTAAACCTGATACTCCACAACCAT	105	0.13253894120320114	No Hit
TACAAGTGATGTGCATCTTTCTTAGTGATTAGCATGTATGTGGTCTTTACT	103	0.13001438994218778	No Hit
AAGTTAAACTCTGCTTCAAAAGCAACTTTAAGGAAATATTCATCCTGTCTC	102	0.1287521143116811	No Hit
GTATGAATACACAAGGGTAAAAGGAGGTCTTGAATCCGCTGTTCGTATTTC	100	0.12622756305066773	No Hit
AGTAACAACTTCACAAGAAACATCTACATCAGTAGTTGCTAAGAAAAGTAC	100	0.12622756305066773	No Hit
TACCAATACAGAGAATGACGAAACCAGAGTGACAGAAGAAGTATTTCAAAT	99	0.12496528742016105	No Hit
ATGTATCCTGTGTATTTGCTCCACATCAAGAGATCACTACAACTACATTGT	98	0.1237030117896544	No Hit
AACCCTTTATCAGATATGTCATTTGCAAATATCTTCTTCCATTTTGTTGCC	98	0.1237030117896544	No Hit
CAATTATGTGTATCACAACATGAAAGATTTTAAACAAACACGTTTGTTCAA	97	0.1224407361591477	No Hit
GAAATGGGTATTGATAAAAAAGTCATCAAGGAAGGAAATGGAACAACTTTC	96	0.12117846052864105	No Hit
TATTCGAACAGTGTTGATTACAACAAAAGAACAAGGTACGCGGTTATAAAC	96	0.12117846052864105	No Hit
AGTATTACCAGCAATTTCCTAATGGATATAGGAAGCAAGAGGTAGTTCTAA	95	0.11991618489813435	No Hit
GTATATCTCATACTCAAGCAAAACAAGTAGTCAATAGAAACTGTCATACTT	95	0.11991618489813435	No Hit
GAAAAAAATGTACAAGGATGCGACAGTTCACTTTGAAATGTAACAAGATAC	95	0.11991618489813435	No Hit
TTTTCTTTGTTTACCACAACAGCTACTACTTGGAGGTTAAGAAACCAACAA	93	0.117391633637121	No Hit
GTCATGATACGTTCATAATTGAACCCCTTATCATACATGCTCAATCTAAAG	92	0.11612935800661432	No Hit
ATTTTACATCGACTTTTTCGTCTGTTTTTTTATCTTCCTTCTTGTCTTCTT	91	0.11486708237610765	No Hit
TGTTAATTGTATCTAAGGTACTACACCACACTTGTTGTCAATATATGCTGT	90	0.11360480674560097	No Hit
CAGAATGTTATGAAGAGCAACTAAATCATGTAGGTAATAAAGTACTTAGCA	90	0.11360480674560097	No Hit
TTCTACATCATCATACTCTGGATAATCAATGTTAACTTCAACTTGGGCGAG	89	0.11234253111509429	No Hit
AACATTCTGAAATGAAAAGTCCACAGGTATTTTGTTCACCACAGTACATGA	89	0.11234253111509429	No Hit
GTTAAAGATAACAGCTTAATTGTGGTTGGTGATGTTAAATCTCGCTCATTT	88	0.11108025548458762	No Hit
TTACTGGATTCCATAATGAGAAATTATACTCAACGAGTCTTATCGCTGAAG	88	0.11108025548458762	No Hit
ATTTGCATCAGTACCTGAACTTTCAAAAGCATAATCTCTACAGCATATGGA	87	0.10981797985408093	No Hit
ACATTAGTATCTGTTTCGCTAATGGAAAAAAAATCCAAAGATTGTCACTGT	87	0.10981797985408093	No Hit
TGTAAGTGCTGTATAAAGCATTTCTCTTGACAGTAAAAAGCAAGGATTAGG	86	0.10855570422357427	No Hit
GTGATATCATTTGTTCTTAGTACTCTCCATTGACTGTGAAAATAGTCTAAA	86	0.10855570422357427	No Hit
GCATACTTGAAAATCATAAATCATTTTCGCCCTTTTGTTCCTGCACAAAAA	86	0.10855570422357427	No Hit
ATTGAATGGTATCACATTTCTTACCAATTTCTTACCATTGTTTGTGCGAAT	86	0.10855570422357427	No Hit
GTTGTTGTTTTGTTCTTTGGGTTTTTTGGTTTTGCTTGTTTGTTTGTTTGT	85	0.10729342859306758	No Hit
AAGCAATACGCTTATCAATATGTACGCTTTACAAATCAAGAAAATCAAAGC	85	0.10729342859306758	No Hit
GAAAAGCACAATGTAAAATGTAAGATGGTAAAAAATGTCTGACTGTATCAA	85	0.10729342859306758	No Hit
CAGTATTTAAGAATCCCATATCGAAACTAGCATTATGCGCTACTAAAATAG	85	0.10729342859306758	No Hit
AATGTAGACTTATAGAAACCACAGGTACTTCGAAAAAGAGTGGAACACAAA	84	0.1060311529625609	No Hit
TTTTAAGATTGATGAAAAGCTTTGATCTAAGATACCTCAAGGGGATTTTTC	84	0.1060311529625609	No Hit
GCATTAGAAGATATCTTAAGACAAGCTTCTTGAAAACATGAATGGTACAGT	84	0.1060311529625609	No Hit
TTTCTGTATCCATGTTTTCTCATTGTTCAATTCCTAACTATGAGTGAGAGC	84	0.1060311529625609	No Hit
GGATACAAGAATATTAATCCACATACCACTTGAACTTTCATGTTTAACAGT	83	0.10476887733205423	No Hit
GAATGATACTTTGTCTATTTCGGTTGGAAAAATTGTATTCAGAGATCGAAA	83	0.10476887733205423	No Hit
ATATTGGGTAGCAACAAGTAATTGTTGCGTTAAATTGTCAAGACTGTTTAA	83	0.10476887733205423	No Hit
ACCAATCAAGATTGATAACTCTTGATCTTTCAGACAACAAAATTGAAGGAG	83	0.10476887733205423	No Hit
ATCTGTGCTTACATAAAAGGTATAGCTACTGTCTTTTTGTAACACCAAAAA	83	0.10476887733205423	No Hit
CTATATGATTGTTCTCATTACTACCACTACTATTCCTAAAGCATCTGACAG	82	0.10350660170154755	No Hit
TCTCAGTACACTTCTTTTCATACCTCAAGAGACTAAAACTAGTTTGAGGAG	81	0.10224432607104088	No Hit
CTTTAAATCTATCCAAATGTCCACTTGCAGATTCAACAAAAAGTGTTTTTC	81	0.10224432607104088	No Hit
GAATGTATGTACTAAGTGATCAGATACTTGTTGCTCAGTCATACCTTCAAT	81	0.10224432607104088	No Hit
AAAGTATAGTGATACGGCTACCAAAGTAACCAGCATGCTACTCAGTTTAAT	81	0.10224432607104088	No Hit
AAGAGGAATTGACCAAAAATGTCACAAAGATTGTTTTGGGGTGAGGAAAAT	81	0.10224432607104088	No Hit
ATTCAACACCAAAGATGGTTCATTAAAATTAGTACCGCTGTTTGATATGAG	81	0.10224432607104088	No Hit
GAGAAAGAGAAAGAAAGACAAGCAATACTAAACAGCAGCAAATGTACATTA	80	0.1009820504405342	No Hit
ACAGTAATGAAAAAGGACAGCAAAATGAACGAACAAAACTCAGCTTTGATA	80	0.1009820504405342	No Hit
GATTGATGGTAGTAGTTTTCATCTTTTGAATTCCTTTCTACATTCCTTTCT	80	0.1009820504405342	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGA	20	6.9935306E-4	45.000004	18
CTCAACG	20	6.9935306E-4	45.000004	29
CGAGTCT	20	6.9935306E-4	45.000004	34
TACCAGC	20	6.9935306E-4	45.000004	6
GCTGGGT	20	6.9935306E-4	45.000004	39
CGTCTGG	20	6.9935306E-4	45.000004	30
TAAGTCG	20	6.9935306E-4	45.000004	25
GCTGACA	20	6.9935306E-4	45.000004	29
TTATCGC	20	6.9935306E-4	45.000004	40
CTGACAT	20	6.9935306E-4	45.000004	30
ATCACAC	20	6.9935306E-4	45.000004	39
GAGACTA	20	6.9935306E-4	45.000004	29
TCGTGTC	20	6.9935306E-4	45.000004	45
TACGATT	20	6.9935306E-4	45.000004	22
GTCGTCT	20	6.9935306E-4	45.000004	28
AACTGGC	20	6.9935306E-4	45.000004	18
ATTGGGG	20	6.9935306E-4	45.000004	29
CTCCTAC	20	6.9935306E-4	45.000004	12
ACGAGTC	20	6.9935306E-4	45.000004	33
CTCTCCT	20	6.9935306E-4	45.000004	10

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content