FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_51.351000000431a2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_51.351000000431a2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50808
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC33046.502912927098095No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT12282.4169422138245946No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG2660.5235396000629823No Hit
AGTCTAGATGGTAAACACTGTTAGCAATTTAAGTGACTATCCTCTAACTGT750.14761454888993858No Hit
ATCATATGGTTGGTAGTCAACAATTTGAATTTCACATTGTTGGTATCAAGA740.14564635490473943No Hit
TTTGTGGTATATCACTTCCTACACTGTGTAGAATCTGTCTCTTATACACAT730.14367816091954022No Hit
TGCATACCTAGTGTCTAGAAGAATAGTACCTAACACATTAGAGTCTGTCTC710.13974177294914186No Hit
CAATACCAGTGTCAGTAACATGAAGAACATTGTTTTCTTTGTCAACCTTCT660.12990080302314597No Hit
CAATAGAAATCTTATCTCCATGTCTTCACATACTTCCTCATCTTCATTTTG610.12005983309715006No Hit
GTTAGAATACGTTCCTGTAACAATATGACTAAGGTACATTCATGTAACGAT600.11809163911195086No Hit
TTTGTGTACCTTTGAAATACATAATTGCTGCATAGTATGGTAAATGTAGCC590.1161234451267517No Hit
CTATCAGGTGTCAGAAATGTTCTTGGTATTTCTCACTTGGTGTAGAACTTC580.1141552511415525No Hit
AAAGTGATGACAACAATGATGATGAAGCTTTACAACTACATAGTCTGTCTC580.1141552511415525No Hit
TTCTTACAGATCCTTTTTTAACAACCCCAAGATGTAATCCAGTTTTACTGT570.11218705715635334No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC570.11218705715635334No Hit
AAAGAGTCATAATGACTACAAAACCAGGCTGGATTTGTATTCTCTCATCGC570.11218705715635334No Hit
ATGTACAAAAATCACAAGCATTCTTATACACCAATAACCTGTCTCTTATAC570.11218705715635334No Hit
ATAGATATCATGATGACACTAAACTCATATCTGTCAATAGTAACTCTGAAC570.11218705715635334No Hit
ACTTTCTTTCACTTAGCATAATGCTTTCAAGACTCATCTACGTTGTACTAC570.11218705715635334No Hit
GTCATTCAGTATTAAGATTCTGAGATTCACACACATTGTTGTACCATAGAT560.11021886317115415No Hit
GAATGGAATGGAATCAAATGGAAAGGAAAGAAATGGAATGGAATGCAATGG560.11021886317115415No Hit
CTATTATTTCTCTGACCACCAAATAATCTACTGTTCCTGTCTCTTATACAC540.10628247520075577No Hit
ATGTAACATCTTATTAGGCACAGTGAAGCATTAAGATTTGATCTGTCTCTT530.10431428121555661No Hit
GTTGTATAGTTACTCACTGTGTTTTCAGTGCTACTAACGTCAATATCCTGT520.10234608723035743No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGGAGAAGAAGAAGAAGAA520.10234608723035743No Hit
TTAAAGTACCAGTTTACAACAACACATGTGCATAGTATCTGTCTCTTATAC510.10037789324515824No Hit
ATCTAGTAGTGTATCTTTCAGTTGTAGTTCTTTGTGCTGTCTCTTATACAC510.10037789324515824No Hit
GTAGTAGGCATTGTTTTAGTCTAAGCATCTTCATTTGTTCAAATCTCACCA510.10037789324515824No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGCA206.9697335E-445.00000410
GATTCTG206.9697335E-445.00000416
ATGCATG206.9697335E-445.0000041
TCCCGTT206.9697335E-445.00000436
AGATTCT206.9697335E-445.00000415
TGCCGTC3650.045.045
TGCACAG250.00208824736.05
TAAATGC250.00208824736.03
ATCTACA250.00208824736.01
TTGAGAC250.00208824736.030
CAATAGA250.00208824736.01
TAGTAGG250.00208824736.02
CCATATT250.00208824736.08
ATGCCGT4600.035.70652444
GTATGCC4800.034.21875442
TATGCCG4800.034.21875443
CGTATGC4800.034.21875441
TCTCGTA4750.034.10526738
TCGTATG4750.034.10526740
CTCGTAT4800.033.75000439
Produced by FastQC (version 0.11.2)