FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_50.35100000043188.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_50.35100000043188.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19106
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC600131.408981471788966No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT345018.057154820475244No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG6523.4125405631738723No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC490.2564639380299382No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC430.22506019051606826No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA310.16225269548832827No Hit
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT240.12561499005547996No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTAT220.1151470742175233No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAA206.860307E-445.03
TGCCGTC5900.044.61864545
TACGGGA301.09844965E-437.4999962
ATGCCGT9400.028.0053244
AAGTACT607.654808E-626.2499984
AGTACTC607.654808E-626.2499985
TATGCCG10400.025.31249843
CTATCTC10400.025.31249835
TCTCGTA10500.025.28571538
TATCTCG10500.025.28571536
TCGTATG10500.025.28571540
CTCGTAT10500.025.28571539
GTATGCC10550.025.16587642
ATCTCGT10550.025.16587637
CGTATGC10550.025.16587641
CCTATCT10500.025.0714334
AACCTAT10500.025.0714332
ACCTATC10500.025.0714333
TAACCTA10550.024.95260631
ATAACCT10650.024.71831130
Produced by FastQC (version 0.11.2)