FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_5.35100000042bee.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_5.35100000042bee.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107529
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC44124.103079169340364RNA PCR Primer, Index 27 (95% over 21bp)
GAGATGAAGAGTTTGATAAAAAGGATAAAGGGACATGGATAGTTCATTCTG18401.7111662900240865No Hit
CTATATAGGAAGACATAGTGATTAAGATGTTAGGTTTTCTAAACTGTTGCA11291.0499493159984747No Hit
ATGTTAAGATATCACAATAAGTGCTCTGTTTACTTGGAAACAATTTTTAGG10811.0053101953891508No Hit
GGATACAACAAAACAAAAAGCAAACAAACGAAAAACAATCAACAACAATAA7720.7179458564666276No Hit
ACTCAGAATAGCTTGAAACTGGAAATAACCCAAATGTCTATCAAATGATAA7600.7067860763142966No Hit
TAGTTATGATGGCGATAATAACATTTGCTTGTACTTTTGTGGGATTCATTT7420.6900464060858001No Hit
AGTATGTACAGGAAATTAAAGTTTTGTTTGAGACTTCATAAGCTTGGTGCA6720.624947688530536No Hit
AAATAAAGCGTTGAATCCTAGATTTCCAAGAAAGGCAATGAATGTGAAGAG6390.5942582931116257No Hit
AATCTATTTATCAATCAGGTTATTGCGGCAAAAAAGTATAATTTCAATGAG5810.5403193557086926No Hit
ATATTACATTTGAGTCTATCGAAGCCTATGAAAACTTCTTTAGTGAAGGAA5170.4808005282295939No Hit
GAATAATATCAACATTCTAGTCCTCTTTTATTCAACTAGCTGTTGATACTT5100.47429065647406743No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGT5080.47243069311534563No Hit
TAATAGGGTTATTATCAGATGGTGGTGTACATGCACATATGAACCATATAA5040.46871076639790193No Hit
GTATAATATTATGTCCAGCATTGATATAGGTTAATTCCTGACGGGTAAAAT5020.4668508030391801No Hit
ATATATAGCTGAGAACTGTAAAACAATTTTATCGATTTACCATCATAACAA4610.42872155418538255No Hit
AAGCTATACAGTATTCCAAATGTTTGCAAGTTTAAAGGATAACAGAACTAA4590.4268615908266607No Hit
TCTATACACTAACAATGAGACTGAAGAAAGAGAAATTAAGGAGTCAATCCC4520.4203517190711343No Hit
ATCGTATGCAAGAGGAAAAAGATCATGCTGTTAAACAAAAAACAGAACCTA4330.40268206716327687No Hit
GTTCAGTAATTCTATTGAGCATTATGGAAAAGTCATCACAGATAGTGAATT4320.40175208548391594No Hit
TTGGTAAAGAAATATGTCACATACATAATGCTAGTGTTAGGGGTAGAAAGT4260.3961721954077505No Hit
CTTTTACATTGTAGTTAAAAGCTTGAACAAACAACTATTACACCTCCTTAA4200.39059230533158495No Hit
GCATATAGATGGATCTTGGTTTTTGTTTTTTGTTTTTTAATCCATTCTGAT4110.3822224702173367No Hit
CGTATACGTTACAGAATAACTACTTGGTTTTAGAAAGAGGAAGTGCTCAAG3880.36083289159203563No Hit
GGAATAATACTAAATGTCCATCTAGAGAAAACTGAATAAACTATCCCCAGA3570.33200345953184723No Hit
ATAGTATTCCATTTCATGAATATGCCACATTTTGTTTATCCATTCATCATT3470.3227036427382381No Hit
ATAAATATGAGTCCCATGATATTTGACTGTTGCTTTCCTTGTAATGAATGT3450.32084367937951624No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3400.3161937709827116No Hit
TAAAAGCTCTCAATTTTAAATGACTTTAATTCGAAAACAAAAACCCTTTGC3210.29852411907485427No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA3200.29759413739549334No Hit
TTAAAAGTAAGAACAAGCAAAGATTAAACCTTGTACCTTTTGCATAATGAA3060.2845743938844405No Hit
AGGTATAAGATTACCAAACAAATAACTCAGAATAATCGTCATAAAGATGCT3040.28271443052571865No Hit
GATATACACTCAAGATGTATTAGAATTCGAAGTTATGGTGCAAACATTAGA2960.2752745770908313No Hit
GTACACAATTACCGTATCATTCATAGAAAAACCAATTACCGTTAATACCGC2940.27341461373210946No Hit
ACCTATATCCTGATAATTAGCAGTTGCAATACTTGGATCATAAGCGATCTC2920.2715546503733876No Hit
ATATAGTAAAAAGGCAATGCAGATTATGTAACATGTAATTACAGTTAGGCT2910.2706246686940267No Hit
ATCCTAAAAGATTACCTGTGTATGTTCTGTAGCAAAGAGATTCTTGATTTT2910.2706246686940267No Hit
CAGTAGTACTGTTCTATGATGCAAAAGAGTTATGGAAAAATAGAAACAGGG2890.2687647053353049No Hit
CAAATATACCCACCAATAAAAACATATCTTCTGACGAAAGCAACATAAACG2810.26132485190041754No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2730.25388499846553025No Hit
AAATATGAAAGAAAAACGTAGTAATGTTCGTTGGTATTTTGCAATAGGGTT2660.2473751267100038No Hit
TGCTTATACTGTGTAAAGCTTTCTATTAATTCGGGGTGATGTAATACTTCT2650.2464451450306429No Hit
TTATCACTTTGTGCTTTTGTCATCAATAACTTTTTTTCCCCCCTAGGATTC2640.24551516335128198No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2610.2427252183131992No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2600.24179523663383828No Hit
AAATAAGTGATTCTAAAGAAGGTATGGGAACCAGCACAGTTGAATTAATCT2490.23156543816086825No Hit
AATATGAATACCCCTGTCATTTACGATACATGTTTTGATAACATCCTTACC2340.2176157129704545No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2310.21482576793237174No Hit
ATAGAATTTATCTACGTACTTTTGCGTAGTTCTGCTATTTTTTATTTTTGA2290.2129658045736499No Hit
CAAGAGATTAAAAGAAATCAATTGCTATAGAGGAATTAGACATAAAAGAGG2240.2083158961768453No Hit
AAGTGGAAGAAAGGATATCAGTGATTGTAGGTAAAGTGAATAAAATGAAGC2190.20366598778004072No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2180.20273600610067982No Hit
TTGATAAATTTCAACACTGGATTTATGAACACCCAAATCATACATTGGAAG2180.20273600610067982No Hit
ACATTACCAAACATATGATTCAGCCTTCTAGGAAAGTTTTGGAAATACCCT2180.20273600610067982No Hit
ATTAATACTAGGCATGAAAAGCGAATTAATTGTTGGTAAAAACGAAGTAAC2160.20087604274195797No Hit
ATATCTGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGG2140.19901607938323612No Hit
GATTGACATTATGACAAAACAATATTAGCATAAACTGAGGACTTCTGTTTA2120.19715611602451433No Hit
GAATATATGTTGAAGTTTTACAACTCCATAAGACAAATAACTCACAACAAA2110.1962261343451534No Hit
TAAAAGATGTGAAGAAACCTTAAATGCATTTTACTAAATGAAAGAAGCCAA2090.19436617098643158No Hit
GTGAATGTATAGTTTACTACGTCACCCACTTTTTCAACTTCCTTAACATCA2090.19436617098643158No Hit
CTAAAGAAGAAACATGCTGATGTCATTATATATCCCTCACTCTGATGAAAG2010.18692631755154424No Hit
ACTGTAGTGTTTTAAAGGTGTATCTAAGAAATTTTCTACGCCGCTAAATTC2000.1859963358721833No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA1970.18320639083410056No Hit
TTTCTTATTAGATAGAATCTAGGTGGAACTGTCAATCAAATTCCTTGTACT1950.18134642747537874No Hit
TACTTAATGTATTTACTTTCCATCTCTTTACCATTTGTCATGGCAACAATT1930.1794864641166569No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1910.17762650075793507No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1890.17576653739921325No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1880.17483655571985232No Hit
GTTTAGAAAATATCAAGATGGATAAATTTCATGTCTTGGAAGTTTGTTGTT1880.17483655571985232No Hit
TATACACACAATGTGATACCATTCAGCCATAACAAAATAATGAAATCCTGT1860.1729765923611305No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1840.17111662900240865No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1830.17018664732304775No Hit
GAAGTAATCATATGTGTTCTTGGGTTGTAATAAGTGTTTGTAGAGAGTAGG1820.16925666564368683No Hit
CTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTTATAA1810.1683266839643259No Hit
GAAACACTTGAATATGGCTTTATTGATGAAATTATGGCCAACAATTCATTG1720.15995684885007766No Hit
ACGTATACTAGGTTTTTTTTTTTTTTTTTCGCAGCTAGTGTAAATGGAATT1710.15902686717071673No Hit
TATTGATACCGCTTACTTTATGTTAGGTTTGGAGAAAGTGGATCCTGATTA1680.156236922132634No Hit
GTATAAGTATCCTTACCGCATTTAATGGATTACTGATGTTATTTCTGGGGA1620.1506570320564685No Hit
TTATGTTATTTTGCTATGAACCAGCAATAAGAAGAAATAGACTCTTCAGTT1610.14972705037710757No Hit
TTCTTAGTCTCTACTTGATGAGACATTGTCATCATACTTTACTTCATTTAG1600.14879706869774667No Hit
GTGTAATTGTATAAATAACAGTCATTGACAACAAAAAATGCATACACAAAC1590.14786708701838575No Hit
ATATAGTGCTCAAATTTTTCTCACAAAAACCTTTTCTTCACTTTCTCATAT1540.14321717862158115No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCG1540.14321717862158115No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA1520.14135721526285933No Hit
TTTTAAAGCATCATTTATTCCTTTGCTCTGTCTTTTTTTAAGCTTTTATTT1520.14135721526285933No Hit
CTTATAGCTTCTTCAGACAATTCGGTATAGTTTCCTTTATCTAAATACCAG1510.1404272335834984No Hit
ATATAGATTTAAAGTCACTTCACATGATGATGATGATGATGATGATGATGA1510.1404272335834984No Hit
TTTTTACACAATGTACAATTCCTTTCCATCAAGATCTTCATTAGCTTATGA1510.1404272335834984No Hit
TATCAACAAACTCCTTTTCCATTTTATATCTTCCTTCCAAAATTTTTGCAA1480.13763728854541565No Hit
GAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1440.13391736182797198No Hit
GTGGCATTTCTATACACTAACAATGAGACTGAAGAAAGAGAAATTAAGGAG1430.13298738014861106No Hit
GTTTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTTG1400.13019743511052834No Hit
TGAGAGCTTTTTACAATTTTACAACAAAAATTGCAGGAATACAAGAGGTAA1390.1292674534311674No Hit
TTCTTGTTCTACTTGAATCTAATTTTTCCATTCGTTCTAGAAGTTCTTAAC1380.1283374717518065No Hit
TATTAGAGAGAAACAAAAATCTTCATTAATTGCAACTTATGTGATTTCCTA1360.12647750839308466No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA1350.12554752671372374No Hit
ATAAAATCTGCTATTTTATAAGAGAGGCACAGAAATAGGCTTCACTAATTG1340.12461754503436283No Hit
GAATCGTTTTGTAACCATGTTTATTGGTATCATTGATTTTACCCGTCAGGA1330.1236875633550019No Hit
ATTCTGTAAGATAATTCTTAAAGCCTTTACATGCAAATTTTTTGAAGCACA1330.1236875633550019No Hit
TTTTCAATCTTATTCTCTACAGACGCCATTAGCACATTATCATTCAAGTTC1320.12275758167564099No Hit
ACAATATTTAGCTAGTAAGAACCATGTCAAATAACTACTTTCTTCTGGAAT1300.12089761831691914No Hit
ATATCTAACTGATCACAAAGAATTTGGTATAAGATTCCGTTAATAAGCGGA1290.11996763663755824No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA1280.11903765495819732No Hit
GTTATGTTGATTTCAGGTATACAATACAGTGATTCAACAATTCTATGCATT1270.11810767327883641No Hit
TGTCCATAACAATAGATATTACTTGGTAGTTATTGACCGAATAGATGCCCC1270.11810767327883641No Hit
ACGTATACTAGGTTTTTTTTTTTTTTTTCGCAGCTAGTGTAAATGGAATTG1250.11624770992011456No Hit
ATCTTCTATGTACCAGATTTTATTAGCAACCTTTGATACAAAGTGTCTATC1230.11438774656139274No Hit
TTAGAATAATGCTTGTATTTCTGGATTGGCATCGATTTCTATTGAGACTGG1210.11252778320267091No Hit
GGTAATATGAACATGTGATTTAAGGTGAAAATCTTCTTTCAATGGCCGTGA1190.11066781984394908No Hit
GTGTATGAAATATCTCAATAAAGCTGATATTTTGAAAATCACTGAGTCTGG1190.11066781984394908No Hit
AAGGTAAGAGAAGTATTTAGAAGACGTTTGAATGCCTTATTGATAGCGCCA1180.10973783816458815No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1170.10880785648522726No Hit
CAGTAGTACTGTTCTATGATTCAAAAGAGTTATGGAAAAATAGAAACAGGG1140.1060179114471445No Hit
ATAATAAACTACTCAAGGTTACTGGAGAGATTAAATGAGTAATACATCTGT1140.1060179114471445No Hit
ATAAAAGGGAATACACGAAATACTAAATTCTCATCCTTAAACAAGTATCTG1120.10415794808842264No Hit
GTATACAAGCAAACTGAATTCAGCAACACTTTGAAAAGATCATTCATCATG1110.10322796640906175No Hit
TATTTCAACTCCTTTAAATGTAATCCACATAAGTACCTTTTCCACCAAAAT1090.10136800305033991No Hit
GTATATTGCCCTGGGTAACATTGACATTTTCACAATATTAATTCTGCCAAT1080.10043802137097899No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCAAT253.858438E-545.0172230
GAGACTT502.1827873E-1145.0172230
ATATCCC253.858438E-545.0172230
ATCCCTC253.858438E-545.0172232
TTTATCG502.1827873E-1145.0172228
AACCTTT502.1827873E-1145.0172228
TTCGGGG502.1827873E-1145.0172230
TGCTAGT502.1827873E-1145.0172229
GCTGTTA551.8189894E-1245.0172227
GATTCCG253.858438E-545.0172233
GTTCTGC206.991554E-445.01721629
AGTCAAC206.991554E-445.01721630
ACACCCA206.991554E-445.01721629
ATAATCG302.141469E-645.01721632
TAGGTAA206.991554E-445.01721628
TGAACAC206.991554E-445.01721626
GTTAGAA206.991554E-445.01721629
GGGACAT2500.045.01721630
GTTCGTT302.141469E-645.01721626
TGCCTTA206.991554E-445.01721633
Produced by FastQC (version 0.11.2)