FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l01n01_bir_1228_pcontrolb_49.3510000004316e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l01n01_bir_1228_pcontrolb_49.3510000004316e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128509
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC1562612.159459648740555No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT92067.1637005968453575No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCG15221.1843528468823195No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC3920.3050370012995199No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC2910.22644328412795992No Hit
AAAAAGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGCCCACG2170.16885976857651994No Hit
TATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCACG1430.11127625302507996No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA1320.10271654125391996No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATTAG206.998244E-445.0151821
TAATCAC207.0116844E-444.9976736
AGGAGGT207.0116844E-444.9976741
ACGTTGC207.0116844E-444.9976727
TCATTCG207.0116844E-444.9976713
GAGTATG207.0116844E-444.9976720
CGTTGCA207.0116844E-444.9976728
GAGTAAG207.0116844E-444.997677
TCCCTAA207.0116844E-444.9976732
GTTAGGT207.0116844E-444.9976728
AGCGGAG207.0116844E-444.9976718
CACTCAG207.0116844E-444.9976721
TCGCTTA207.0116844E-444.9976712
ACTCGGT207.0116844E-444.9976733
GCGGAGG207.0116844E-444.9976719
ACTCAGG207.0116844E-444.9976722
CGGAGGA207.0116844E-444.9976720
ACCTTCC207.0116844E-444.9976728
TGGTCGC207.0116844E-444.997679
CGCTTAA207.0116844E-444.9976713
Produced by FastQC (version 0.11.2)